Asian-Australasian Journal of Animal Sciences

  • 제32권3호
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  • Pages.350-356
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  • 2019
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  • 1011-2367(pISSN)
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  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Effects of exercise on myokine gene expression in horse skeletal muscles

  • Lee, Hyo Gun (Department of Animal Science, College of Natural Resources and Life Sciences, Pusan National University) ;
  • Choi, Jae-Young (Department of Animal Science, College of Natural Resources and Life Sciences, Pusan National University) ;
  • Park, Jung-Woong (Department of Animal Science, College of Natural Resources and Life Sciences, Pusan National University) ;
  • Park, Tae Sub (Graduate School of International Agricultural Technology and Institute of Green-Bio Science and Technology, Seoul National University) ;
  • Song, Ki-Duk (Department of Animal Biotechnology, Chonbuk National, University) ;
  • Shin, Donghyun (Department of Animal Biotechnology, Chonbuk National, University) ;
  • Cho, Byung-Wook (Department of Animal Science, College of Natural Resources and Life Sciences, Pusan National University)
  • 투고 : 2018.05.16
  • 심사 : 2018.09.13
  • 발행 : 2019.03.01
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초록

Objective: To examine the regulatory effects of exercise on myokine expression in horse skeletal muscle cells, we compared the expression of several myokine genes (interleukin 6 [IL-6], IL-8, chemokine [C-X-C motif] ligand 2 [CXCL2], and chemokine [C-C motif] ligand 4 [CCL4]) after a single bout of exercise in horses. Furthermore, to establish in vitro systems for the validation of exercise effects, we cultured horse skeletal muscle cells and confirmed the expression of these genes after treatment with hydrogen peroxide. Methods: The mRNA expression of IL-6, IL-8, CXCL2, and CCL4 after exercise in skeletal muscle tissue was confirmed using quantitative-reverse transcriptase polymerase chain reactions (qRT-PCR). We then extracted horse muscle cells from the skeletal muscle tissue of a neonatal Thoroughbred. Myokine expression after hydrogen peroxide treatments was confirmed using qRT-PCR in horse skeletal muscle cells. Results: IL-6, IL-8, CXCL2, and CCL4 expression in Thoroughbred and Jeju horse skeletal muscles significantly increased after exercise. We stably maintained horse skeletal muscle cells in culture and confirmed the expression of the myogenic marker, myoblast determination protein (MyoD). Moreover, myokine expression was validated using hydrogen peroxide ($H_2O_2$)-treated horse skeletal muscle cells. The patterns of myokine expression in muscle cells were found to be similar to those observed in skeletal muscle tissue. Conclusion: We confirmed that several myokines involved in inflammation were induced by exercise in horse skeletal muscle tissue. In addition, we successfully cultured horse skeletal muscle cells and established an in vitro system to validate associated gene expression and function. This study will provide a valuable system for studying the function of exercise-related genes in the future.

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참고문헌

  1. Poole DC. Current concepts of oxygen transport during exercise. Equine Comp Exerc Physiol 2004;1:5-22. https://doi.org/10.1079/ECP20036
  2. Kim H, Lee T, Park W, et al. Peeling back the evolutionary layers of molecular mechanisms responsive to exercise-stress in the skeletal muscle of the racing horse. DNA Res 2013;20:287-98. https://doi.org/10.1093/dnares/dst010
  3. Park KD, Park J, Ko J, et al. Whole transcriptome analyses of six thoroughbred horses before and after exercise using RNASeq. BMC Genomics 2012;13:473. https://doi.org/10.1186/1471-2164-13-473
  4. Capomaccio S, Cappelli K, Barrey E, et al. Microarray analysis after strenuous exercise in peripheral blood mononuclear cells of endurance horses. Anim Genet 2010;41:166-75. https://doi.org/10.1111/j.1365-2052.2010.02129.x
  5. Eivers SS, McGivney BA, Fonseca RG, et al. Alterations in oxidative gene expression in equine skeletal muscle following exercise and training. Physiol Genomics 2010;40:83-93. https://doi.org/10.1152/physiolgenomics.00041.2009
  6. Park JW, Song KD, Kim NY, et al. Molecular analysis of alternative transcripts of equine AXL receptor tyrosine kinase gene. Asian-Australas J Anim Sci 2017;30:1471-7. https://doi.org/10.5713/ajas.17.0409
  7. Cho HW, Shin S, Park JW, et al. Molecular characterization and expression analysis of the peroxisome proliferator activated receptor delta ($PPAR{\delta}$) gene before and after exercise in horse. Asian-Australas J Anim Sci 2015;28:697-702. https://doi.org/10.5713/ajas.14.0575
  8. Park JW, Choi JY, Hong SA, et al. Exercise induced upregulation of glutamate-cysteine ligase catalytic subunit and glutamatecysteine ligase modifier subunit gene expression in Thoroughbred horses. Asian-Australas J Anim Sci 2017;30:728-35. https://doi.org/10.5713/ajas.16.0776
  9. Pedersen BK, Akerstrom TC, Nielsen AR, Fischer CP. Role of myokines in exercise and metabolism. J Appl Physiol 2007;103:1093-8. https://doi.org/10.1152/japplphysiol.00080.2007
  10. Jonsdottir I, Schjerling P, Ostrowski K, et al. Muscle contractions induces interleukin-6 mRNA production in rat skeletal muscles. J Physiol (Lond) 2000;528:157-63. https://doi.org/10.1111/j.1469-7793.2000.00157.x
  11. Akerstrom TC, Steensberg A, Keller P, et al. Exercise induces interleukin-8 expression in human skeletal muscle. J Physiol 2005;563:507-16. https://doi.org/10.1113/jphysiol.2004.077610
  12. Ostrowski K, Rohde T, Asp S, Schjerling P, Pedersen BK. Chemokines are elevated in plasma after strenuous exercise in humans. Eur J Appl Physiol 2001;84:244-5. https://doi.org/10.1007/s004210170012
  13. Peake JM, Roberts LA, Figueiredo VC, et al. The effects of cold water immersion and active recovery on inflammation and cell stress responses in human skeletal muscle after resistance exercise. J Physiol 2017;595:695-711. https://doi.org/10.1113/JP272881
  14. Croisier JL, Camus G, Venneman I, et al. Effects of training on exercise-induced muscle damage and interleukin 6 production. Muscle Nerve 1999;22:208-12. https://doi.org/10.1002/(SICI)1097-4598(199902)22:2<208::AID-MUS8>3.0.CO;2-B
  15. Pedersen BK, Steensberg A, Fischer C, et al. The metabolic role of IL-6 produced during exercise: is IL-6 an exercise factor? Proc Nutr Soc 2004;63:263-7. https://doi.org/10.1079/PNS2004338
  16. Wolsk E, Mygind H, Grondahl TS, Pedersen BK, van Hall G. IL-6 selectively stimulates fat metabolism in human skeletal muscle. Am J Physiol Endocrinol Metab 2010;299:E832-40. https://doi.org/10.1152/ajpendo.00328.2010
  17. Steensberg A. The role of IL-6 in exercise-induced immune changes and metabolism. Exerc Immunol Rev 2003;9:40-7.
  18. Koch AE, Polverini PJ, Kunkel SL, et al. Interleukin-8 as a macrophage-derived mediator of angiogenesis. Science 1992;258:1798-801. https://doi.org/10.1126/science.1281554
  19. Catoire M, Mensink M, Kalkhoven E, Schrauwen P, Kersten S. Identification of human exercise-induced myokines using secretome analysis. Physiol Genomics 2014;46:256-67. https://doi.org/10.1152/physiolgenomics.00174.2013
  20. Bystry RS, Aluvihare V, Welch KA, Kallikourdis M, Betz AG. B cells and professional APCs recruit regulatory T cells via CCL4. Nat Immunol 2001;2:1126-32. https://doi.org/10.1038/ni735
  21. Zlotnik A, Yoshie O. Chemokines: a new classification system and their role in immunity. Immunity 2000;12:121-7. https://doi.org/10.1016/S1074-7613(00)80165-X
  22. Yahiaoui L, Gvozdic D, Danialou G, Mack M, Petrof BJ. CC family chemokines directly regulate myoblast responses to skeletal muscle injury. J Physiol 2008;586:3991-4004. https://doi.org/10.1113/jphysiol.2008.152090
  23. Warren GL, O'Farrell L, Summan M, et al. Role of CC chemokines in skeletal muscle functional restoration after injury. Am J Physiol Cell Physiol 2004;286:C1031-6. https://doi.org/10.1152/ajpcell.00467.2003
  24. Pourteymour S, Eckardt K, Holen T, et al. Global mRNA sequencing of human skeletal muscle: search for novel exerciseregulated myokines. Mol Metab 2017;6:352-65. https://doi.org/10.1016/j.molmet.2017.01.007
  25. Beavers KM, Brinkley TE, Nicklas BJ. Effect of exercise training on chronic inflammation. Clin Chim Acta 2010;411:785-93. https://doi.org/10.1016/j.cca.2010.02.069
  26. Niess AM, Simon P. Response and adaptation of skeletal muscle to exercise-the role of reactive oxygen species. Front Biosci 2007;12:4826-38. https://doi.org/10.2741/2431
  27. Peake J, Nosaka K, Suzuki K. Characterization of inflammatory responses to eccentric exercise in humans. Exerc Immunol Rev 2005;11:64-85.
  28. Seale P, Sabourin LA, Girgis-Gabardo A, et al. Pax7 is required for the specification of myogenic satellite cells. Cell 2000;102:777-86. https://doi.org/10.1016/S0092-8674(00)00066-0

피인용 문헌

  1. Comparison for immunophysiological responses of Jeju and Thoroughbred horses after exercise vol.33, pp.3, 2019, https://doi.org/10.5713/ajas.19.0260
  2. Validation of exercise-response genes in skeletal muscle cells of Thoroughbred racing horses vol.34, pp.1, 2019, https://doi.org/10.5713/ajas.18.0749
  3. Epigenetic control of exercise adaptations in the equine athlete: Current evidence and future directions vol.53, pp.3, 2019, https://doi.org/10.1111/evj.13320
  4. Regulation of toll-like receptors expression in muscle cells by exercise-induced stress vol.34, pp.10, 2021, https://doi.org/10.5713/ab.20.0484