Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Effect of IgY on Periodontitis and Halitosis Induced by Fusobacterium nucleatum

  • Wang, Fang (Shanghai University of Medicine and Health Sciences) ;
  • Qiao, Wu (College of Food Science and Technology, Shanghai Ocean University) ;
  • Bao, Bin (College of Food Science and Technology, Shanghai Ocean University) ;
  • Wang, Shujun (Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Huaihai Institute of Technology) ;
  • Regenstein, Joe Mac (Department of Food Science, Cornell University) ;
  • Shi, Yilei (Shanghai MAXAM Company Limited) ;
  • Wu, Wenhui (College of Food Science and Technology, Shanghai Ocean University) ;
  • Ma, Ming (College of Food Science and Technology, Shanghai Ocean University)
  • Received : 2018.10.24
  • Accepted : 2018.12.28
  • Published : 2019.02.28
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Abstract

Fusobacterium nucleatum is a morbific agent in periodontitis and halitosis. Egg yolk antibody (IgY) was obtained from egg yolks from chickens stimulated with F. nucleatum. This study was to assess the effectiveness of IgY on periodontitis and halitosis caused by F. nucleatum in vitro and in vivo. The growth of F. nucleatum was inhibited (p <0.05) by different concentrations of IgY in vitro and the results of a Halimeter show volatile sulfur compounds (VSCs) were reduced to $904{\pm}57ppb$ at a concentration 40 mg/ml of IgY. The changes of fatty acids of F. nucleatum were determined using GC-MS. The scores for odor index of rat saliva were decreased. The major constituent of volatile organic compounds (VOCs) including short-chain acids decreased 46.2% in 10 mg/ml IgY, ammonia decreased 70% in 40 mg/ml IgY, while aldehydes and olefine ketones were almost unchanged. The ELISA assay revealed that IL-6 and TNF-${\alpha}$ were decreased after 4 weeks' IgY treatment. Morphometric (X-ray) and histological analyses (HE) showed that IgY reduced alveolar bone loss and collagen fibers became orderly in rat models. As a result, IgY may have the potential to treat periodontitis and halitosis.

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