한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제47권1호
  • /
  • Pages.158-163
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  • 2019
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  • 1598-642X(pISSN)
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  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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A New ELISA Kit Based on Antigenic Epitopes for Diagnosing Brucella abortus

  • Khodabakhsh, Tannaz (Department of Microbiology, School of Biology Sciences, North Branch of Islamic Azad University) ;
  • Arabi, Azin (Department of Microbiology, School of Biology Sciences, North Branch of Islamic Azad University) ;
  • Pakzad, Parviz (Department of Microbiology, School of Biology Sciences, North Branch of Islamic Azad University) ;
  • Gheflat, ShivaSadat (Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences) ;
  • Bahreinipour, Akram (Iran Veterinary Organization (IVO)) ;
  • Bandehpour, Mojgan (Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences)
  • 투고 : 2018.06.22
  • 심사 : 2018.09.27
  • 발행 : 2019.03.28
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초록

Brucellosis is one of the most important zoonotic diseases that lead to a great amount of economic losses. Prevention and diagnosis are both necessary to eradicate this disease. The identification and evaluation of different antigens of Brucella spp. play a key role in the progress of diagnostic programs. In this study, we designed, produced, and evaluated a 24-kDa polypeptide containing antigenic epitopes of VirB2, 3, and 9 of Brucella abortus for use with the ELISA kit. The produced polypeptide is appropriate for diagnosing brucellosis in bovines by a laboratory diagnostic kit, with 100% sensitivity and 97.5% specificity.

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참고문헌

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