Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Biosynthesis of Chondroitin in Engineered Corynebacterium glutamicum

  • Cheng, Fangyu (Department of Chemical Engineering, Tsinghua University) ;
  • Luozhong, Sijin (Department of Chemical Engineering, Tsinghua University) ;
  • Yu, Huimin (Department of Chemical Engineering, Tsinghua University) ;
  • Guo, Zhigang (Department of Chemical Engineering, Tsinghua University)
  • Received : 2018.10.31
  • Accepted : 2019.01.17
  • Published : 2019.03.28
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Abstract

Chondroitin, the precursor of chondroitin sulfate, which is an important polysaccharide, has drawn significant attention due to its applications in many fields. In the present study, a heterologous biosynthesis pathway of chondroitin was designed in a GRAS (generally recognized as safe) strain C. glutamicum. CgkfoC and CgkfoA genes with host codon preference were synthesized and driven by promoter Ptac, which was confirmed as a strong promoter via GFPuv reporter assessment. In a lactate dehydrogenase (ldh) deficient host, intracellular chondroitin titer increased from 0.25 to 0.88 g/l compared with that in a wild-type host. Moreover, precursor enhancement via overexpressing precursor synthesizing gene ugdA further improved chondroitin titers to 1.09 g/l. Chondroitin production reached 1.91 g/l with the engineered strain C. glutamicum ${\Delta}L-CgCAU$ in a 5-L fed-batch fermentation with a single distribution $M_w$ of 186 kDa. This work provides an alternative, safe and novel means of producing chondroitin for industrial applications.

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References

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