Korean Journal of Acupuncture

Society for Meridian and Acupoint (경락경혈학회)
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Toxicity Evaluation of TA, a Pharmacopuncture Medicine, in an in Vivo Micronucleus Test

ICR 마우스를 이용한 소핵시험을 통한 TA 약침의 안전성 평가

  • Hwang, Ji Hye (Department of Acupuncture & Moxibustion Medicine, College of Korean Medicine, Gachon University) ;
  • Jung, Hyo Won (Department of Herbology, College of Korean Medicine, Dongguk University) ;
  • Jung, Chul (Namsangcheon Korean Medicine Clinic)
  • 황지혜 (가천대학교 한의과대학 침구의학과) ;
  • 정효원 (동국대학교 한의과대학 본초학교실) ;
  • 정철 (남상천 한의원)
  • Received : 2019.02.12
  • Accepted : 2019.03.19
  • Published : 2019.03.27
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Abstract

Objectives : TA, a polyherbal extract, typically is used for pharmacopuncture therapy on patients with traffic accident-related injuries and musculoskeletal diseases. This study was performed to evaluate the safety of the TA extract, using a micronucleus test. Methods : The dose range and sampling time were first established. An in vivo micronucleus test was then performed to determine the induction of micronuclei in mouse bone marrow cells after a single intramuscular administration of TA to 7-week-old ICR mice (0.2 ml/animal, at 24 hours post-dosing). Results : The incidence of micro-nucleated polychromatic erythrocytes (PCEs) in PCEs in the TA group was similar to that in the negative-control group, while that in the positive-control group was significantly greater. The positive- and negative-control groups did not differ in the ratio of PCEs to total erythrocytes. Conclusions : Our toxicity study indicates that the TA extract does not induce micronucleus formation in mouse bone marrow cells.

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