Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Identification of the active components inhibiting the expression of matrix metallopeptidase-9 by TNFα in ethyl acetate extract of Euphorbia humifusa Willd

  • Ahn, Seunghyun (Division of Bioscience and Biotechnology, BMIC, Konkuk University) ;
  • Jung, Hyeryoung (Division of Bioscience and Biotechnology, BMIC, Konkuk University) ;
  • Jung, Yearam (Division of Bioscience and Biotechnology, BMIC, Konkuk University) ;
  • Lee, Junho (Division of Bioscience and Biotechnology, BMIC, Konkuk University) ;
  • Shin, Soon Young (Department of Biological Sciences, Sanghuh College of Life Sciences, Konkuk University) ;
  • Lim, Yoongho (Division of Bioscience and Biotechnology, BMIC, Konkuk University) ;
  • Lee, Young Han (Department of Biological Sciences, Sanghuh College of Life Sciences, Konkuk University)
  • Received : 2019.05.27
  • Accepted : 2019.10.23
  • Published : 2019.12.31
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Abstract

Euphorbia humifusa Willd (EuH), called Ttang-Bin-Dae in Korea, is a traditional medicinal plant widely used for its anti-inflammatory and antiviral activity. Ethyl acetate (EA) extracts of EuH (EA/EuH) inhibit invasion and metastasis by inhibiting tumor necrosis factor TNFá-induced matrix metalloproteinases (MMP)-9 expression in human breast cancer cells. However, the bioactive components of EA/EuH mediating the inhibition of MMP-9 expression have not been identified. In the present study, three bioactive constituents of EA/EuH were isolated using high-performance liquid chromatography. Nuclear magnetic resonance spectroscopy revealed isoquercetin, avicularin, and astragalin as the bioactive compounds responsible for preventing TNFα-induced MMP-9 mRNA expression in breast cancer cells. These findings suggest that isoquercetin, avicularin, and astragalin could be used as valuable anti-metastatic agents against metastatic cancers.

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References

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