Journal of Korean Society of Occupational and Environmental Hygiene (한국산업보건학회지)

Korean Industrial Hygiene Association (한국산업보건학회)
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Acute Toxicity Study of the Glyoxal by Intratracheal Instillation in Male Sprague-Dawley Rats

수컷 랫드(Sprague-Dawley)에서 글리옥살(glyoxal)의 단회 기도내 투여에 따른 급성 독성시험

  • Kim, Hyeon-Young (National Center for Efficacy Evaluation of Respiratory Disease Product, Korea Institute of Toxicology) ;
  • Kim, Kicheon (National Center for Efficacy Evaluation of Respiratory Disease Product, Korea Institute of Toxicology) ;
  • Kim, In-Hyeon (National Center for Efficacy Evaluation of Respiratory Disease Product, Korea Institute of Toxicology) ;
  • Kim, Min-Seok (National Center for Efficacy Evaluation of Respiratory Disease Product, Korea Institute of Toxicology) ;
  • Kim, Sung-Hwan (National Center for Efficacy Evaluation of Respiratory Disease Product, Korea Institute of Toxicology) ;
  • Lee, Kyuhong (National Center for Efficacy Evaluation of Respiratory Disease Product, Korea Institute of Toxicology)
  • Received : 2019.09.10
  • Accepted : 2019.11.05
  • Published : 2019.12.31
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Abstract

Objectives: The present study was performed to obtain acute toxicity information on glyoxal in male rats after intratracheal instillation. Methods: In order to calculate the LD50 of glyoxal using Probit analysis with SAS, the test article was one intratracheal instillation to male Sprague-Dawley rats at dose levels of 0, 225, 451 or 902 mg/kg. During the test period, mortality, clinical signs, and body and organ weights were examined. At the end of the 14-day observation period, all animals were sacrificed and complete gross postmortem and histopathological examinations were performed. Results: Four animals of the 902 mg/kg group died within one week after the administration of glyoxal. All treatment group in a dose dependent manner, decreased body weight was found during the study period. The absolute and relative lung weight, and histopathological changes (bronchiolar-alveolar hyperplasia, chronic inflammation) of lung exhibited an increased in glyoxal treated groups in a dose dependent manner. However, there were no changes on the organ weights and histopathological changes of any other organ except lung. Conclusions: The results obtained in the present study suggest that the LD50 in male Sprague-Dawley rats after a single intratracheal instillation of glyoxal was considered to be 866.9 mg/kg and the lung was found to be the target organ for glyoxal.

Keywords

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