한국작물학회지 (KOREAN JOURNAL OF CROP SCIENCE)

  • 제64권4호
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  • Pages.373-383
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  • 2019
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  • 0252-9777(pISSN)
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  • 2287-8432(eISSN)
한국작물학회 (The Korean Society of Crop Science)
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야생 호밀 염색체 첨가 밀 계통의 단백질 발현 양상 비교 분석

Identification of the Protein Function and Comparison of the Protein Expression Patterns of Wheat Addition Lines with Wild Rye Chromosomes

  • 이대한 ((주)대유 마케팅부) ;
  • 조건 (한국기초과학지원연구원 연구장비운영부) ;
  • 우선희 (충북대학교 식물자원학과) ;
  • 조성우 (경남과학기술대학교 농학.한약자원학부)
  • Lee, Dae Han (DAEYU Co., LTD) ;
  • Cho, Kun (Center for Research Equipment, Korean Basic Science Institute) ;
  • Woo, Sun Hee (Department of Crop Science, Chungbuk National University) ;
  • Cho, Seong-Woo (Department of Agronomy & Medicinal Plant Resources, Gyeongnam National University of Science and Technology)
  • 투고 : 2019.10.14
  • 심사 : 2019.10.25
  • 발행 : 2019.12.31
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초록

야생 호밀 염색체 첨가 계통의 단백질 발현 양상을 보통 밀과 비교함으로써 발현의 차이를 보이는 단백질의 기능을 동정함으로써 야생 호밀의 작물학적 유용 가치를 확인하고자 이 연구를 수행하였다. 전반적으로 야생 호밀 염색체 첨가계통은 보통 밀의 유전적 배경을 바탕으로 건조와 열에 대한 비생물학적 스트레스에 대한 저항성 관련 단백질과 바이러스성 병원균에 대한 저항성 관련 단백질 및 척박한 환경에 적응하는 생리대사에 관련된 단백질을 가지고 있는 것을 확인하였다. 하지만 아직 야생 호밀의 단백질 기능에 대한 정보와 작물학적 이용에 대한 연구가 미흡한 상태이다. 앞으로 국내 야생 호밀의 유용 유전자원으로써의 작물학적 이용과 기능에 대한 지속적인 연구가 필요하다.

The objectives of this study were to compare the protein expression patterns and degrees and identify the protein function of disomic addition lines (DAs) in Leymus racemosus, in order to improve the quality of wheat. Upon SDS-PAGE, L. racemosus showed two major protein bands whereas Chinese Spring (CS) had four major protein bands of high molecular weight. The DA(s) generally showed a similar protein expression pattern to that of CS, because 42 chromosomes were from CS and two chromosomes were from L. racemosus. However, only the L.r[J] line showed two protein bands of between 15 and 20 kDa, like L. racemosus. Image analysis based on 2-DE revealed that L.r[F] had the most upregulated protein spots, whereas L.r[N] had the least upregulated protein spots. For L.r[I], the frequency of the downregulated protein spots was higher than that of the upregulated ones. Using MALDI-TOF MS, the protein function was identified for each protein spot on the 2-DE polyacrylamide gel. The protein spots were classified into 11 groups according to protein function. Among the 11 groups, most protein spots of the DA(s) were identified as proteins related to metabolism. Additionally, unique protein spots of the DA(s) were related to abiotic stressors such as cold and heat. Those proteins are useful for improving wheat quality with resistance against abiotic stressors.

키워드

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