한국콘텐츠학회논문지 (The Journal of the Korea Contents Association)

  • 제19권1호
  • /
  • Pages.242-255
  • /
  • 2019
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  • 1598-4877(pISSN)
  • /
  • 2508-6723(eISSN)
한국콘텐츠학회 (The Korea Contents Association)
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치주염 원인균에 대한 천연 식물 추출물의 항균효과

Antimicrobial Effect of Natural Plant Extracts against Periodontopathic Bacteria

  • 이승희 (대구보건대학교 치기공과) ;
  • 김민정 (대구보건대학교 치기공과)
  • 투고 : 2018.10.15
  • 심사 : 2018.12.20
  • 발행 : 2019.01.28
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초록

이 연구는 34종의 천연 식물 추출물을 이용하여 치주염 원인균 Actinobacillus actinomycetemcomitans와 Prevotella intermedia에 대한 항균효과를 조사하였다. 이에 본 연구는 치주염 원인균에 대한 천연 식물 추출물의 항균효과를 연구하고자 유기용매 추출물을 이용하여 생육저지활성을 측정하고, 시료 추출물의 최소생육저해농도(Minimum Inhibition Concentration, MIC)를 측정한다. 1차적으로 34종의 각 천연 식물 추출물을 에탄올로 추출하여 디스크 확산법(disk diffusion method)을 이용하여 각 추출물의 생육저지환(clear zone, mm)의 크기를 측정한 결과 나복자, 목통, 백작약, 사간, 선복화, 어성초, 연교, 진교, 천련자, 황금, (천)황련, 황백, 해동피 등 13종에서 천연 식물 추출물이 치주염의 원인균인 A. actinomycetemcomitans에 대해서 생육저지 활성을 나타내었다. P. intermedia의 경우, 나복자, (일)당귀, 목통, 사간, 선복화, 어성초, 육계, 자완, 천련자, 황금, (천)황련, 황백, 해동피 등 13종에서 생육저지 활성을 나타내었다. A. actinomycetemcomitans에 대해서는 사간, 육계, 해동피, 황백, (천)황련에서 항균효과가 나타났다. (천)황련은 A. actinomycetemcomitans의 모든 유기용매 분획에서 가장 우수한 생육저지 활성을 나타내었다. P. intermedia는 사간, 육계, 천련자, 황백, 황련에서 생육저지 활성이 나타났다.

In this study, we examined the antimicroboal effect against Actinobacillus actinomycetemcomitans and Prevotella intermedia which were the bacteria causing the Periodontopathic by using 34 types of natural plant extracts. Therefore, this study measures growth inhibition activity and Minimum Inhibition Concentration (MIC) of a sample extract with the use of organic solvent extracts in order to analyze the antibacterial effect of natural plant extracts on periodontopathic bacteria. Each of the 34 types of natural plant extracts were extracted by using the ethanol, and subsequently, the size of growth inhibition zone(clear zone, ㎜) of respective extracts were measured through the disk diffusion method. As a result, it was found that the growth inhibitory activity was found for A. actinomycetemcomitans, which is the bacteria causing the Periodontitis, in 13 types of natural plant extracts such as Raphanus sativus, Akebia quinata, Paeonia lactiflora, Belamcanda chinensis, Inula britannics, Houttuynia cordata, Forsythia saxatilis, Gentiana macrophylla, Melia azedarach, Scutellaria baicalensis, Coptis chinensis, Phellodendron amurense, Kalopanax Pictus, etc. In the case of P. intermedia, the growth inhibitory activity was found in 13 types of natural plant extracts such as Raphanus sativus, Angelica acutiloba, Akebia quinata, Belamcanda chinensis, Inula britannics, Houttuynia cordata, Cinnamomum cassia, Aster tataricus, Melia azedarach, Scutellaria baicalensis, Coptis chinensis, Phellodendron amurense, Kalopanax Pictus etc. For A. actinomycetemcomitans, anti-bacterial effect was exhibited in Belamcanda chinensis, Cinnamomum cassia, Kalopanax Pictus, Phellodendron amurense, Coptis chinensis. The Coptis chinensis showed the most excellent growth inhibitory activity in all organic solvent fragment, while P. intermedia showed the growth inhibitory activity in Belamcanda chinensis, Cinnamomum cassia, Meliaazedarach, Phellodendron amurense, and Coptis chinensis.

키워드

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Fig. 1. Ethanol extraction procedure of Natural plant

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Fig. 2. Organic solvents fractionation procedure from Ethanol extracts

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Fig. 3. Minimum inhibition concentration assay

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Fig. 4. Cell growth of A. Actinomycetemcomitans

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Fig. 5. Cell growth of P. intermedia

Table 1. 34 List Natural plant tested in this experiment

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Table 2. Lists of Periodontopathogens used for antimicrobial activity test and their relations to oxygen

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Table 3. Yield of Ethanol extracts from Natural plan

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Table 4. Inhibition zone of Natural plant extracts against A. actinomycetemcomitans

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Table 5. Inhibition zone of Natural plant extracts against P. intermedia

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Table 6. Antimicrobial activity of ethanol extracts and organic solvent fractions from Belamcanda chinensis, Cinnamomum cassia, Melia azedarach Kalopanax Pictus, Phellodendron amurense, Coptis chinensis against A. actinomycetemcomitans and P. intermedia causing peridontal disease.

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Table 7. Minimum Inhibition Concentration of Belamcanda chinensis, Kalopanax pictus, Coptis chinensis, Scutellaria baicalensis, Aster tataricus ethanol extracts against A. actinomycetemcomitans and P. intermediacausing periodontal disease

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