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Genetic relatedness of Megalocytivirus from diseased fishes in Korea

국내 어류에서 분리된 Megalocytivirus의 유전형 분류 및 상관관계 분석

  • Lee, Eun Sun (Pathology Research Division, National Institute of Fisheries Science) ;
  • Cho, Miyoung (Pathology Research Division, National Institute of Fisheries Science) ;
  • Min, Eun Young (Pathology Research Division, National Institute of Fisheries Science) ;
  • Jung, Sung Hee (Pathology Research Division, National Institute of Fisheries Science) ;
  • Kim, Kwang Il (Pathology Research Division, National Institute of Fisheries Science)
  • 이은선 (국립수산과학원 병리연구과) ;
  • 조미영 (국립수산과학원 병리연구과) ;
  • 민은영 (국립수산과학원 병리연구과) ;
  • 정승희 (국립수산과학원 병리연구과) ;
  • 김광일 (국립수산과학원 병리연구과)
  • Received : 2019.11.15
  • Accepted : 2019.12.11
  • Published : 2019.12.31

Abstract

In this study, we collected 39 megalocytiviruses isolated from diseased fish in Korea from 2012 to 2018. Major capsid protein (MCP) gene, a part of vascular endothelial growth factor (VEGF) gene and histidine triad motif-like protein (HIT) genes of Megalocytivirus were targeted for PCR amplification and analysis of those DNA nucleotide sequences. Korean strains revealed two genotypes (red sea bream iridovirus and turbot reddish body iridovirus types) based on the phylogeny of MCP gene. The red sea bream iridovirus type (RSIV-type) megalocytiviruses were divided into RSIV-subgroup 1 and 2. From the phylogenetic analysis of the VEGF genes, a genotypic variant of RSIV-type Megalocytivirus was identified. The HIT-like protein gene was detected in RSIVs, but not in TBRIV and ISKNV, suggesting that HIT-like protein gene may be specific in RSIV.

본 연구에서는 2012년부터 2018년 1월까지 국내 어류에서 참돔이리도바이러스병 (red sea bream iridoviral disease; RSIVD) 으로 확정 진단된 주요 분리주에 대하여 major capsid protein (MCP) 유전자의 염기서열을 바탕으로 Megalocytivirus의 유전적 관계를 명확히 했다. 또한, Megalocytivirus와 숙주 세포간 상호작용에 영향을 줄 수 있는 특이 유전자 2종, Vascular endothelial growth factor (VEGF) 및 Histidine triad motif인 HIT-like 단백질 (HIT)에 대한 염기서열 분석을 통해 유전적 상관관계를 고찰하였다. 국내 어류에서 분리된 39개의 megalocytiviruses는 2가지 유전형인 red sea bream iridovirus (RSIV)형과 turbot reddish body iridovirus (TRBIV)형으로 분류되었으며, RSIV형의 megalocytiviruses는 다시 유전아형인 RSIV-subgroup 1형과 2형으로 분류되었다. 그리고 VEGF 유전자 염기서열 분석 결과에서는 RSIV형의 바이러스에서 변이가 발생되었음을 확인할 수 있었으며, HIT-like 단백질 유전자는 RSIV형의 Megalocytivi rus에서만 발견되는 것을 알 수 있었다.

Keywords

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