한국버섯학회지 (Journal of Mushroom)

  • 제17권4호
  • /
  • Pages.275-283
  • /
  • 2019
  • /
  • 1738-0294(pISSN)
  • /
  • 2288-8853(eISSN)
한국버섯학회 (The Korean Society of Mushroom Science)
권호 표지
DOI QR코드

DOI QR Code

해조류 기반 엘리시터 처리에 의한 꽃송이버섯의 GABA 함량 증가 및 흥분성 신경세포의 수상돌기 발달 억제

Enhanced GABA content from sodium alginate-induced Sparassis latifolia influences dendrite development in primary cortical neurons

  • 최문희 (조선대학교 공학기술연구원) ;
  • 기성환 (조선대학교 약학대학) ;
  • 이성은 (조선대학교 약학대학) ;
  • 이금화 (조선대학교 약학대학) ;
  • 신현재 (조선대학교 생명화학고분자공학과)
  • Choi, Moon-Hee (The Research Institute of Advanced Engineering Technology, Chosun University) ;
  • Ki, SungHwan (College of Pharmacy, Chosun University) ;
  • Lee, Seong-Eun (College of Pharmacy, Chosun University) ;
  • Lee, GumHwa (College of Pharmacy, Chosun University) ;
  • Shin, Hyun-Jae (Department of Biochemical Polymer Engineering of Chosun University)
  • 투고 : 2019.12.17
  • 심사 : 2019.12.20
  • 발행 : 2019.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Sparassis latifolia is a fungus abundant in β-glucan and amino acids and is highly valued as a medicinal mushroom. Among amino acids, γ-aminobutyric acid (GABA) is a free amino acid and has biological effects, such as increase/decrease of hypertension, improvement of cerebral blood flow, and prevention of dementia. In this study, biological elicitors were used to increase bioactive substances as a biofortification method. Sodium alginate extracted from seaweed (Sargassum horneri, Sargassum fulvellum, Sargassum fusiforme) were used as the elicitor. The levels of β-glucan and GABA in the mycelium and fruiting body grown by adding the elicitor to the medium were investigated. Addition of sodium alginate positively affected GABA production and negatively affected the β-glucan production in these fungi. Sodium alginates extracted from S. fulvellum induced the highest increase in GABA in the mycelium and fruiting bodies. Moreover, we investigated the effects of the extracts from mycelium and fruiting bodies on dendrite development in primary cortical neurons. We found that the extract from the fruiting bodies of sodium alginate treated fungi with increased levels of GABA inhibited the dendrite outgrowth of excitatory neurons, but not inhibitory neurons.

키워드

참고문헌

  1. Anderson RJ, Simpson AC, Channon S, Samuel M, Brown RG. 2013. Social problem solving, social cognition, and mild cognitive impairment in Parkinson's disease. Behav Neurosci 127: 184-192. https://doi.org/10.1037/a0030250
  2. Choi MH, Han HK, Lee YJ, Jo HG, Shin HJ. 2014. In vitro anti-cancer activity of hydrophobic fractions of Sparassis latifolia extract using AGS, A529, and HepG2 cell lines. J Mushrooms 12: 304-310. https://doi.org/10.14480/JM.2014.12.4.304
  3. Jo HG, Shin HJ. 2017. Effect of addition amino acids on the mycelial growth and the contents of $\beta$-glucan and $\gamma$-aminobutyric acid (GABA) in Sparassis latifolia. J Mushrooms 15: 38-44. https://doi.org/10.14480/JM.2017.15.1.38
  4. Jung YS, Park SJ, Kim JE, Yang SA, Park JH, Kim JH, Jhee KH, Lee SP, Lee IS. 2012. A comparative study of GABA, glutamate contents, acetylcholinesterase inhibition and antiradical activity of the methanolic extracts from 10 edible plants. Korean J Food Sci Technol 44: 447-451. https://doi.org/10.9721/KJFST.2012.44.4.447
  5. Kaplan DR, Miller FD. 2000. Neurotrophin signal transduction in the nervous system. Curr Opin Neurobiol 10: 381-391. https://doi.org/10.1016/S0959-4388(00)00092-1
  6. Kim HJ, Choi SH, Chun BG, Kim DH. 2011. The role of adipose tissue vasculature in energy balance. J Korean Soc Pediatr Endocrinol. 16: 139-156. https://doi.org/10.6065/jkspe.2011.16.3.139
  7. Kim JY, Lee SH. 1993. Effects of amino acids and some drugs related to GABA receptors on the neuronal activity in isolated medullary dorsal horn of rat. J Korean Assoc Oral Maxillofac Surg 19: 401-411.
  8. Ko SJ, Shin YJ, Jang WS, Ha YJ, Lee SA, Ahn MS, Kwon OS, Shin SH. 2010. Protective effects of Chungsimyeonjaeum on glutamate-induced apoptosis in C6 glial cells. Korean J Intern Med 31: 54-65. https://doi.org/10.3904/kjim.2016.31.1.54
  9. Kontos HA. 1989. Oxygen radicals in CNS damage. Chem-Biol Interact 72: 229-255. https://doi.org/10.1016/0009-2797(89)90001-X
  10. Li N, Liu Y, Zhao Y, Zheng X, Lu J, Liang Y. 2016. Simultaneous HPLC determination of amino acids in tea infusion coupled to pre-column derivatization with 2, 4-dinitrofluorobenzene. Food Anal Methods 9: 1307-1314. https://doi.org/10.1007/s12161-015-0310-8
  11. McAllister AK, Lo DC, Katz LC. 1995. Neurotrophins regulate dendritic growth in developing visual cortex. Neuron 15: 791-803. https://doi.org/10.1016/0896-6273(95)90171-X
  12. Naveh-Benjamin M, Guez J, Kilb A, Reedy S. 2004. The associative memory deficit of older adults: further support using face-name associations. Psychol Aging 19: 541-546. https://doi.org/10.1037/0882-7974.19.3.541
  13. Niu S, Renfro A, Quattrocchi CC, Sheldon M, D'Arcangelo G. 2004. Reelin promotes hippocampal dendrite development through the VLDLR/ApoER2-Dab1 pathway, Neuron 41: 71-84. https://doi.org/10.1016/S0896-6273(03)00819-5
  14. Oh DS, Kim HS, Shim BS, Wui AJ, Yoon BS, Kim KW, Wang SJ. 2013. Effect of mycelial culture of cauliflower mushroom (Sparassis crispa) using LED lighting operation. J Mushrooms 11: 24-31. https://doi.org/10.14480/JM.2013.11.1.024
  15. Ohno N, Furukawa M, Miura NN, Adachi Y, Motoi M, Yadomae T. 2001. Antitumor $\beta$-glucan from the cultured fruit body of Agaricus blazei. Biol Pharm Bull 24: 820-828. https://doi.org/10.1248/bpb.24.820
  16. Podkorytova AV, Vafina LH, Kovaleva EA, Mikhailov VI. 2007. Production of algal gels from the brown alga, Laminaria japonica Aresch., and their biotechnological applications. J Appl Phycol 19: 827-830. https://doi.org/10.1007/s10811-007-9256-6
  17. Ramassamy C. 2006. Emerging role of polyphenolic compounds in the treatment of neurodegenerative diseases: a review of their intracellular targets. Eur J Pharmacol 545: 51-64. https://doi.org/10.1016/j.ejphar.2006.06.025
  18. Ramesh SA, Tyerman SD, Xu B, Bose J, Kaur S, Conn V, Domingos P, Ullah S, Wege S, Shabala S, Feijo JA, Ryan PR, Gilliham M. 2015. GABA signalling modulates plant growth by directly regulating the activity of plant-specific anion transporters. Nat Commun 6: 7879. https://doi.org/10.1038/ncomms8879
  19. Reiner A. 1991. A comparison of neurotransmitter-specific and neuropeptide-specific neuronal cell types present in the dorsal cortex in Turtles with those present in the isocortex in mammals: Implications for the evolution of isocortex. Brain Behav Evol 38: 73-82. https://doi.org/10.1159/000114380
  20. Ryoo R, Sou HD, Ka KH, Park H. 2018. Elicitor-induced $\beta$-glucan contents in fruit body of cauliflower mushroom (Sparassis latifolia). Forest Sci Technol 14: 119-125. https://doi.org/10.1080/21580103.2018.1475307
  21. Sari M, Prange A, Lelley JI, Hambitzer R. 2017. Screening of beta-glucan contents in commercially cultivated and wild growing mushrooms. Food Chem 216: 45-51. https://doi.org/10.1016/j.foodchem.2016.08.010
  22. Sou HD, Ryoo R, Ka KH, Park H. 2017. The mycelial growth and ligninolytic enzyme activity of cauliflower mushroom (Sparassis latifolia). Forest Sci Technol 13: 158-163. https://doi.org/10.1080/21580103.2017.1387612