Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
권호 표지
DOI QR코드

DOI QR Code

Changes in the complete blood count and serum biochemical parameters of Sapsaree dogs according to different age groups

삽살개의 연령에 따른 혈구 및 혈청 생화학 수치 변화 양상 연구

  • Yi, Seung-Won (Division of Animal Diseases & Health, National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Eunju (Division of Animal Diseases & Health, National Institute of Animal Science, Rural Development Administration) ;
  • Oh, Sang-Ik (Division of Animal Diseases & Health, National Institute of Animal Science, Rural Development Administration) ;
  • Ha, Ji-Hong (Korean Sapsaree Foundation) ;
  • Lee, Bugeun (Korean Sapsaree Foundation) ;
  • Yoo, Jae Gyu (Division of Animal Diseases & Health, National Institute of Animal Science, Rural Development Administration) ;
  • Do, Yoon Jung (Division of Animal Diseases & Health, National Institute of Animal Science, Rural Development Administration)
  • 이승원 (농촌진흥청 국립축산과학원 가축질병방역과) ;
  • 김은주 (농촌진흥청 국립축산과학원 가축질병방역과) ;
  • 오상익 (농촌진흥청 국립축산과학원 가축질병방역과) ;
  • 하지홍 (한국삽살개재단) ;
  • 이부근 (한국삽살개재단) ;
  • 류재규 (농촌진흥청 국립축산과학원 가축질병방역과) ;
  • 도윤정 (농촌진흥청 국립축산과학원 가축질병방역과)
  • Received : 2019.11.21
  • Accepted : 2019.12.20
  • Published : 2019.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

The complete blood count and serum biochemical parameters are essential tools for diagnosis and monitoring of physiological and disease conditions in dogs. These parameters may be affected by the breed and age of dogs. This study aimed to compare the changes of complete blood count and serum biochemical parameters according to the increasing age in 156 Sapsarees. In this study, the Sapsarees were clustered into ten age groups: four to six months, seven to nine months, ten to twelve months, one year, two years, three to four years, five to six years, seven to eight years, nine to ten years, and, eleven to twelve years old. In the result of the complete blood count, the total red blood cell count (P<0.001), hematocrit (P<0.001), and hemoglobin (P<0.001) values were significantly increased from 4~6 months to 2 years old Sapsarees. While the mean corpuscular volume (P<0.001) and mean corpuscular hemoglobin (P<0.001) values were significantly increased from 4~6 months to 3~4 years old. The mean corpuscular hemoglobin concentration was significantly (P=0.037) increased from 4~6 to 10~12-month-old Sapsarees. Total white cell count was significantly (P<0.001) decreased from 1 year to 9~10 years old. The result of differential white blood cell count showed that neutrophil count was significantly (P<0.001) increased from the age 4~6 months to 11~12 years old Sapsarees, whereas, lymphocyte (P<0.001), monocyte (P<0.001), and eosinophil (P=0.042) counts were significantly decreased from 7~9 months to 7~8 years, 4~6 months to 9~10 years, 3~4 to 5~6 years old, respectively. In the serum biochemistry result, the creatinine concentration was significantly (P<0.001) increased from 4~6 to 10~12 months, but significantly (P=0.006) decreased from 7~8 to 10~12 years old. Phosphate concentration was significantly (P<0.001) decreased from 3~4 to 9~10 years old, but significantly (P=0.021) increased from 9~10 to 11~12 years old. Calcium concentration significantly (P<0.001) decreased from 10~12 months to 11~12 years old. Total protein concentration significantly (P<0.001) increased from 4~6 months to 2 years old. While concentrations of albumin (P=0.004) and globulin (P<0.001) was significantly increased from 4~6 months to 3~4 years old, and from 4~6 months to 11~12 years old, respectively. The alkaline phosphate concentration was significantly (P<0.001) decreased from 4~6 months to 2 years old. We found that there are differences in the hematological parameters in relation with the increasing age in Sapsaree breed.

Keywords

References

  1. Brenten T, Morris PJ, Salt C, Raila J, Kohn B, Schweigert FJ, Zentek J. 2016. Age-associated and breed-associated variations in haematological and biochemical variables in young labrador retriever and miniature schnauzer dogs. Vet Rec Open, 3: e000166. https://doi.org/10.1136/vetreco-2015-000166
  2. Bush BM. 1991. Alkaline phosphatase. pp. 376-381. In: Interpretation of laboratory results for small animal clinicians. Blackwell Scientific Publication, Oxford, London, England.
  3. Chang YM, Hadox E, Szladovits B, Garden OA. 2016. Serum biochemical phenotypes in the domestic dog. PloS one 11: e0149650. https://doi.org/10.1371/journal.pone.0149650
  4. Choi SY, Hwang JS, Kim IH, Hwang DY, Kang HG. 2011. Basic data on the hematology, serum biochemistry, urology, and organ weights of beagle dogs. Lab Anim Res 27: 283-291. https://doi.org/10.5625/lar.2011.27.4.283
  5. Crincoli CM, Brathwaite WA, Rihner MO, Nikiforov AI, Harris SB, Greeley MA, Eapen AK. 2016. A 90-day dietary study of a (2R, 4R)-monatin salt in Beagle dogs. Food Chem Toxicol 91: 181-190. https://doi.org/10.1016/j.fct.2015.08.020
  6. De Martinis M, Franceschi C, Monti D, Ginaldi L. 2005. Inflammageing and lifelong antigenic load as major determinants of ageing rate and longevity. FEBS Lett 579: 2035-2039. https://doi.org/10.1016/j.febslet.2005.02.055
  7. Dircks BH, Mischke R, Schuberth HJ. 2012. Platelet-neutrophil aggregate formation in blood samples from dogs with systemic inflammatory disorders. Am J Vet Res 73: 939-945. https://doi.org/10.2460/ajvr.73.7.939
  8. Goddard A, Leisewitz AL, Kristensen AT, Schoeman JP. 2015. Platelet activation and platelet–leukocyte interaction in dogs naturally infected with Babesia rossi. Vet J 205: 387-392. https://doi.org/10.1016/j.tvjl.2015.05.008
  9. Harper EJ, Hackett RM, Wilkinson J, Heaton PR. 2003. Age-related variations in hematologic and plasma biochemical test results in Beagles and Labrador Retrievers. J Am Vet Med Assoc 223: 1436-1442. https://doi.org/10.2460/javma.2003.223.1436
  10. Hawkey CM. 1975. Comparative haematology of the vertebrates. pp. 3-15. Comparative Mammalian Haematology. Whitefriars Press Ltd, London.
  11. Ikeuchi J, Yoshizaki T, Hirata M. 1991. Plasma biochemistry values of young beagle dogs. J Toxicol Sci 16: 49-59. https://doi.org/10.2131/jts.16.49
  12. Kaneko JJ, Harvey JW, Bruss ML. 2008. Clinical Biochemistry of Domestic Animals. 6th ed. pp. 889-896. Academic Press, San Diego, CA, USA.
  13. Kaspar LV, Norris WP. 1997. Serum chemistry values of normal dogs(beagles): associations with age, sex, and family line. Lab Anim Sci 27: 980-985.
  14. Kim E, Choe C, Yoo JG, So KM, Jung Y, Cho A, Kim S, Oh SI, Do YJ. 2018. Comparison of hematological and serum biochemical parameters among small breed dogs. Korean J Vet Serv 41: 1-8. https://doi.org/10.7853/kjvs.2018.41.1.1
  15. Lequarre AS, Andersson L, Andre C, Fredholm M, Hitte C, Leeb T, Lohi H, Lindblad-Toh K, Georges M. 2011. LUPA: a European initiative taking advantage of the canine genome architecture for unravelling complex disorders in both human and dogs. Vet J 189: 155-159. https://doi.org/10.1016/j.tvjl.2011.06.013
  16. Levy JK, Crawford PC, Werner LL. 2006. Effect of age on reference intervals of serum biochemical values in kittens. J Am Vet Med Assoc 228: 1033-1037. https://doi.org/10.2460/javma.228.7.1033
  17. Lowseth LA, Gillett NA, Gerlach RF, Muggenburg BA. 1990. The effects of aging on hematology and serum chemistry values in the beagle dog. Vet Clin Pathol 19: 13-19. https://doi.org/10.1111/j.1939-165X.1990.tb00535.x
  18. Lund C, Kuhl S, Mischke R, Gunzel-Apel AR. 2000. Reference values of the red blood profile in beagle, German shepherd and golden retriever puppies. Berl Munch Tierarztl 113: 447-453.
  19. Meuten D. 2012. Parathyroid glands and calcium and phosphorus metabolic pathology. pp. 545-568. In: Thrall MA, Weiser G, Allison RW, Campbell TW(ed.). Veterinary hematology and clinical chemistry. 2nd ed. Wiley-Blackwell, Ames, IA, USA.
  20. Moritz A, Walcheck BK, Weiss DJ. 2005. Evaluation of flow cytometric and automated methods for detection of activated platelets in dogs with inflammatory disease. Am J Vet Res 66: 325-329. https://doi.org/10.2460/ajvr.2005.66.325
  21. Mundim AV, Coelho AO, Hortencio SM, Guimaraes EC, Espindola FS. 2007. Influence of age and sex on the serum biochemical profile of Doberman dogs in the growth phase. Comp Clin Path 16: 41-46. https://doi.org/10.1007/s00580-006-0653-z
  22. Oo T, Kyaw KN, Kyaw ST, Kyi AM, Kyi KT, Khin MM, Khin SK, Khaing TK, Khaing E, Khaing AN, Aung M, Kyaw WO, Po SP. 2017. Age Related Changes in Hematological Values of Myanmar Local Puppies. J Adv Vet Anim Res 7: 116-119.
  23. Park CE, Lee EW, Sung KC, Choi SG. 2010. Investigation of hematological analyses in Gyeongju DongGyeong dog populations. Korean J Vet Serv 33: 367-373.
  24. Pickrell JA, Schluter SJ, Belasich JJ, Stewart EV, Meyer J, Hobbs CH, Jones RK. 1974. Relationship of age of normal dogs to blood serum constituents and reliability of measured single values. Am J Vet Res 35: 897-903.
  25. Rortveit R, Saevik BK, Eggertsdottir AV, Skancke E, Lingaas F, Thoresen SI, Jansen JH. 2015. Age-related changes in hematologic and serum biochemical variables in dogs aged 16-60 days. Vet Clin Pathol 44: 47-57. https://doi.org/10.1111/vcp.12220
  26. Rosol TJ, Capen CC. 1997. Phosphate metabolism. pp. 646-648. In: Kaneko JJ, Harvey JW, Bruss, ML(ed.). Clinical biochemistry of domestic animals. Academic Press, San Diego, CA, USA.
  27. Rosset E, Rannou B, Casseleux G, Chalvet-Monfray K, Buff S. 2012. Age-related changes in biochemical and hematologic variables in Borzoi and Beagle puppies from birth to 8 weeks. Vet Clin Pathol 41: 272-282. https://doi.org/10.1111/j.1939-165X.2012.00415.x
  28. Smets PM, Lefebvre HP, Aresu L, Croubels S, Haers H, Piron K, Meyer E, Daminet S. 2012. Renal function and morphology in aged Beagle dogs before and after hydrocortisone administration. PLoS One 7: e31702. https://doi.org/10.1371/journal.pone.0031702
  29. Weiner DJ, Bradley RE. 1972. The hemogram and certain serum protein fractions in normal beagle dogs. Veterinary Medicine Small Animal Clinician. 67: 393-398.
  30. Weiss DJ, Brazzell JL. 2006. Detection of activated platelets in dogs with primary immune-mediated hemolytic anemia. J Vet Intern Med 20: 682-686. https://doi.org/10.1111/j.1939-1676.2006.tb02915.x
  31. Youn SK, Fujise H, Lee YS. 1999. Studies on hematologic values and serum chemistry of Jindo, Poongsan and Mixed dogs. J Vet Clin 16: 248-252.