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Anti-inflammatory and Immunosuppressive Effects of Panax notoginseng

  • Cao, Thao Quyen (College of Pharmacy, Drug Research and Development Center, Daegu Catholic University) ;
  • Han, Jae Hyuk (College of Pharmacy, Drug Research and Development Center, Daegu Catholic University) ;
  • Lee, Hyun-Su (College of Pharmacy, Drug Research and Development Center, Daegu Catholic University) ;
  • Ha, Manh Tuan (College of Pharmacy, Drug Research and Development Center, Daegu Catholic University) ;
  • Woo, Mi Hee (College of Pharmacy, Drug Research and Development Center, Daegu Catholic University) ;
  • Min, Byung Sun (College of Pharmacy, Drug Research and Development Center, Daegu Catholic University)
  • 투고 : 2019.05.30
  • 심사 : 2019.09.18
  • 발행 : 2019.12.31

초록

Here, we designed to examine the anti-inflammatory effects on RAW264.7 cells and the immunosuppressive effects by evaluating interleukin-2 (IL-2) production in Jurkat T cells using a MeOH extract of Panax notoginseng roots. The results showed that the MeOH extract inhibited the synthesis of nitric oxide (NO) in a dose-dependent manner (IC50 value of 7.08 ㎍/mL) and displayed effects on T cell activation at a concentration of 400 ㎍/mL. In efforts to identify the potent compounds, bioactivity-guided fractionation of the MeOH extract and chemical investigation of its active CH2Cl2-, EtOAc-, and butanol-soluble fractions led to the successful isolation and identification of eleven compounds, including two polyacetylenes (1, 2), a steroid saponin (3), seven dammarane-type ginsenosides (4 - 10), and an oleanane-type ginsenoside (11). Among them, compound 11 was isolated from this plant for the first time. Compound 2 exhibited potent inhibitory effects on NO synthesis and an immunosuppressive effect with IC50 values of 2.28 and 65.57 μM, respectively.

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참고문헌

  1. Medzhitov, R.; Janeway, C. A. Jr. Curr. Opin. Immunol. 1997, 9, 4-9. https://doi.org/10.1016/S0952-7915(97)80152-5
  2. Yang, Z.; Matteson, E. L.; Goronzy, J. J.; Weyand, C. M. Arthritis. Res. Ther. 2015, 17, 29. https://doi.org/10.1186/s13075-015-0542-4
  3. Yu, T. K.; Caudell, E. G.; Smid, C.; Grimm, E. A. J. Immunol. 2000, 164, 6244-6251. https://doi.org/10.4049/jimmunol.164.12.6244
  4. Medzhitov, R.; Janeway, C. A. Jr. Science 2002, 296, 298-300. https://doi.org/10.1126/science.1068883
  5. Xu, W.; Larbi, A. Exp. Gerontol. 2018, 107, 98-101. https://doi.org/10.1016/j.exger.2017.11.018
  6. Wang, C. Z.; McEntee, E.; Wicks, S.; Wu, J. A.; Yuan, C. S. J. Nat. Med. 2006, 60, 97-106. https://doi.org/10.1007/s11418-005-0027-x
  7. Lee, D. G.; Lee, J.; Yang, S.; Kim, K. T.; Lee, S. Nat. Prod. Sci. 2015, 21, 111-121.
  8. Lau, A. J.; Woo, S. O.; Koh, H. L. J. Chromatogr. A. 2003, 1011, 77-87. https://doi.org/10.1016/S0021-9673(03)01135-X
  9. Ng, T. B. J. Pharm. Pharmacol. 2006, 58, 1007-1019. https://doi.org/10.1211/jpp.58.8.0001
  10. Li, S. H.; Chu, Y. Acta Pharmacol. Sin. 1999, 20, 551-554.
  11. Zhao, G. R.; Xiang, Z. J.; Ye, T. X.; Yuan, Y. J.; Guo, Z. X. Food Chem. 2006, 99, 767-774. https://doi.org/10.1016/j.foodchem.2005.09.002
  12. Ahn, K. S.; Noh, E. J.; Zhao, H. L.; Jung, S. H.; Kang, S. S.; Kim, Y. S. Life Sci. 2005, 76, 2315-2328. https://doi.org/10.1016/j.lfs.2004.10.042
  13. El-Desouky, S. K.; Gamal-Eldeen, A. M. Pharm. Biol. 2009, 47, 872-877. https://doi.org/10.1080/13880200902950785
  14. Kim, D. C.; Ko, W.; Ha, T. M..; Nhiem, N. X.; Yen, P. H.; Tai, B. H.; Truong, L. H.; Long, V. N.; Gioi, T.; Hong Quang, T.; Minh, C. V.; Oh, H.; Kim, Y. C.; Kiem PV. Pharm. Biol. 2017, 55, 1195-1201. https://doi.org/10.1080/13880209.2017.1296001
  15. Min, B. S.; Cuong, T. D. Nat. Prod. Sci. 2013, 19, 201-205.
  16. Cao, T. Q.; Tran, M. H.; Kim, J. A.; Tran, P. T.; Lee, J. H.; Woo, M. H.; Lee, H. K.; Min, B. S. Bioorg. Med. Chem. Lett. 2015, 25, 5087-5091. https://doi.org/10.1016/j.bmcl.2015.10.020
  17. Knispel, N.; Ostrozhenkova, E.; Schramek, N.; Huber, C.; Pena-Rodriguez, L. M.; Bonfill, M.; Palazon, J.; Wischmann, G.; Cusido, R. M.; Eisenreich, W. Molecules 2013, 18, 7686-7698. https://doi.org/10.3390/molecules18077686
  18. Lee, J. M.; Lee, D. G.; Lee, K. H.; Cho, S. H.; Nam, K. W.; Lee, S. H. Afr. J. Pharm. Pharmacol. 2013, 7, 294-301. https://doi.org/10.5897/AJPP12.898
  19. Han, M.; Hou, J. G.; Dong, C. M.; Li, W.; Yu, H. L.; Zheng, Y. N.; Chen, L. Molecules. 2010, 15, 399-406. https://doi.org/10.3390/molecules15010399
  20. Ko, S. R.; Choi, K. J.; Suzuki, K.; Suzuki, Y. Chem. Pharm. Bull. 2003, 51, 404-408. https://doi.org/10.1248/cpb.51.404
  21. Yu, H. S.; Zhang, L. J.; Song, X. B.; Liu, Y. X.; Zhang, J.; Cao, M.; Kang, L. P.; Kang, T. G.; Ma, B. P. China J. Chin. Mater. Med. 2013, 38, 3910-3917.
  22. Cho, J. G.; Lee, M. K.; Lee, J. W.; Park, H. J.; Lee, D. Y.; Lee, Y. H.; Yang, D. C.; Baek, N. I. J. Ginseng Res. 2010, 34, 113-121. https://doi.org/10.5142/jgr.2010.34.2.113
  23. Yang, H. J.; Kim, J. Y.; Kim, S. O.; Yoo, Y. H.; Sung, S. H. J. Ginseng Res. 2014, 38, 194-202. https://doi.org/10.1016/j.jgr.2014.05.002
  24. Yoshikawa, M.; Matsuda, H.; Harada, E.; Murakami, T.; Wariishi, N.; Yamahara, J.; Murakami, N. Chem. Pharm. Bull. 1994, 42, 1354-1356. https://doi.org/10.1248/cpb.42.1354
  25. Ngo, Q. T.; Cao, T. Q.; Tran, P. L.; Kim, J. A.; Seo, S. T.; Kim, J. C.; Woo, M. H.; Lee, J. H.; Min, B. S. Bioorg. Med. Chem. Lett. 2018, 28, 2109-2115. https://doi.org/10.1016/j.bmcl.2018.04.023
  26. Robb, R. J.; Munck, A.; Smith, K. A. J. Exp. Med. 1981, 154, 1455-1474. https://doi.org/10.1084/jem.154.5.1455
  27. Hooton, J. W.; Gibbs, C.; Paetkau, V. J. Immunol. 1985, 135, 2464-2473.
  28. Muhlradt, P. F.; Opitz, H. G. Eur. J. Immunol. 1982, 12, 983-985. https://doi.org/10.1002/eji.1830121117
  29. Lotze, M. T.; Frana, L. W.; Sharrow, S. O.; Robb, R. J.; Rosenberg, S. A. J. Immunol. 1985, 134, 157-166.
  30. Shaw, J.; Meerovitch, K.; Bleackley, R. C.; Paetkau, V. J. Immunol. 1988, 140, 2243-2248.
  31. Pereira, R. L.; Ibrahim, T.; Lucchetti, L.; da Silva, A. J.; Goncalves de Moraes, V. L. Immunopharmacology 1999, 43, 31-37. https://doi.org/10.1016/S0162-3109(99)00039-9
  32. Jin, L.; Zhou, W.; Li, R.; Jin, M.; Jin, C.; Sun, J.; Li, G. Nat. Prod. Res. 2019, 1-4. https://doi.org/10.1080/1478641031000111534
  33. Christensen, L. P. Recent Pat. Food Nutr. Agric. 2011, 3, 64-77. https://doi.org/10.2174/2212798411103010064
  34. Chien, S. C.; Tseng, Y. H.; Hsu, W. N.; Chu, F. H.; Chang, S. T.; Kuo, Y. H.; Wang, S. Y. Nat. Prod. Commun. 2014, 9, 1589-1590.
  35. Shim, S. Y.; Sung, S. H.; Lee, M. Pharmacogn. Mag. 2018, 14, 358-362. https://doi.org/10.4103/pm.pm_58_18