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어류병원성 세균 및 C2C12 근원세포에 대한 polyamine 계열 물질인 spermidine의 항균 및 항산화 활성

Antibacterial and Antioxidant Activity of Spermidine, a Natural Polyamine, on Fish Pathogenic Bacteria and C2C12 Myoblast Cells

  • 황보현 (동의대학교 한의과대학 생화학교실 및 항노화연구소) ;
  • 최은옥 (동의대학교 한의과대학 생화학교실 및 항노화연구소) ;
  • 김민영 (동의대학교 한의과대학 생화학교실 및 항노화연구소) ;
  • 지선영 (동의대학교 한의과대학 생화학교실 및 항노화연구소) ;
  • 홍수현 (동의대학교 한의과대학 생화학교실 및 항노화연구소) ;
  • 박철 (동의대학교 자연과학대학 분자생물학과) ;
  • 차희재 (고신대학교 의과대학 기생충학 및 유전학교실) ;
  • 김석만 (부산대학교 자연과학대학 화학과) ;
  • 김희수 (부산대학교 자연과학대학 생명과학과) ;
  • 황혜진 (동의대학교 의료보건생활대학 식품영양학과) ;
  • 최영현 (동의대학교 한의과대학 생화학교실 및 항노화연구소)
  • Hwang-Bo, Hyun (Department of Biochemistry, Dong-eui University College of Korean Medicine, Dong-eui University) ;
  • Choi, Eun-Ok (Department of Biochemistry, Dong-eui University College of Korean Medicine, Dong-eui University) ;
  • Kim, Min Young (Department of Biochemistry, Dong-eui University College of Korean Medicine, Dong-eui University) ;
  • Ji, Seon Yeong (Department of Biochemistry, Dong-eui University College of Korean Medicine, Dong-eui University) ;
  • Hong, Su Hyun (Department of Biochemistry, Dong-eui University College of Korean Medicine, Dong-eui University) ;
  • Park, Cheol (Department of Molecular Biology, College of Natural Sciences, Dong-eui University) ;
  • Cha, Hee-Jae (Department of Parasitology and Genetics, Kosin University College of Medicine) ;
  • Kim, Suhkmann (Department of Chemistry, Pusan National University) ;
  • Kim, Heui-Soo (Department of Biological Sciences, Pusan National University) ;
  • Hwang, Hye-Jin (Department of Food and Nutrition, College of Nursing, Healthcare Sciences & Human Ecology, Dong-eui University) ;
  • Choi, Yung Hyun (Department of Biochemistry, Dong-eui University College of Korean Medicine, Dong-eui University)
  • 투고 : 2019.11.19
  • 심사 : 2019.11.25
  • 발행 : 2019.12.31

초록

We compared the antibacterial activities of spermidine and astaxanthin against two gram-positive bacteria such as Streptococcus parauberis and S. iniae to find new antibacterial candidates. We also evaluated the preventive effects of spermidine against oxidative stress-induced cytotoxicity in C2C12 myoblasts. Our results indicated that spermidine has more significant antibacterial activities than astaxanthin against both two fish pathogenic bacteria as well as gram-negative bacteria Escherichia coli used as a control group. Minimum inhibitory concentration and minimum bactericidal concentration of spermidine were 0.25 mM and 1 mM against S. parauberis, 1 mM and 3 mM against S. iniae, and 0.5 mM and 1.5 mM against E. coli, respectively. In addition, the postantibiotic effect lasted from 7 h, 5 h and 6 h for S. parauberis, S. iniae and E. coli, respectively. The results also showed that the decreased C2C12 cell viability by H2O2 could be attributed to the induction of DNA damage and apoptosis accompanied by the increased production of reactive oxygen species, which was remarkably protected by spermidine. Additionally, the antioxidant effect of spermidine was associated with the activation of Nrf2 signaling pathway. According to the data, spermidine may be a potential lead compound which can be further optimized to discover novel antibacterial and antioxidant agents.

키워드

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