Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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The effect of fenofibrate and 17β-estradiol on lipid metabolism in low-fat diet-fed OVX female mice

저지방식이를 섭취한 난소절제 암컷 쥐에서 지질대사에 대한 fenofibrate과 17β-estradiol의 영향

  • Jeong, Sun-Hyo (Division of Biomedical Engineering & Health, Science Management, Mokwon University)
  • 정선효 (목원대학교 의생명.보건학부)
  • Received : 2019.10.30
  • Accepted : 2019.12.26
  • Published : 2019.12.30
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Abstract

It was studied whether fenofibrate alone or combinational treatments of fenofibrate and 17β-estradiol regulates serum lipid levels, and whether the effects of fenofibrate on serum lipid metabolism are affected by co-administration of 17β-estradiol in low-fat diet-fed ovariectomized female mice. Compared with low-fat diet-fed controls, mice treated with fenofibrate alone and mice treated with fenofibrate and 17β-estradiol didn't decrease body weight at 8 weeks. Fenofibrate alone or combinational treatments of fenofibrate and 17β-estradiol did not regulate serum levels of total cholesterol and HDL-cholesterol. Fenofibrate decreased plasma levels of LDL-cholesterol and triglycerides compared with controls. The combinational treatments of fenofibrate and 17β-estradiol showed more beneficial effects on triglycerides than fenofibrate alone. Therefore, the present study found that serum triglycerides reduced by fenofibtae treatment alone could be more improved by combinational treatments of fenofibrate and 17β-estradiol in low-fat diet-fed ovariectomized female mice.

본 연구는 저지방식이를 섭취한 난소절제 암컷 쥐에서 피노피브레이트(fenofibrate) 단독처리 및 피노피브레이트와 17베타-에스트라다이올(17β-estradiol)과의 동시처리가 혈청 속 지질대사를 조절하는지를 조사하였고, 피노피브레이트와 17베타-에스트라다이올의 동시처리에 의해 혈청 속 지질대사에 대한 피노피브레이트 단독처리의 효과가 어떻게 조절되는지를 연구하였다. 저지방식이만을 섭취한 대조군에 비해 피노피브레이트가 단독처리된 쥐와 피노피브레이트와 17베타-에스트라다이올이 동시처리된 쥐의 8주째 몸무게는 감소되지 않았다. 피노피브레이트 단독처리와 피노피브레이트와 17베타-에스트라다이올 동시처리는 혈청 속 총 콜레스테롤과 HDL-콜레스테롤을 감소시키지 않았다. 피노피브레이트는 대조군에 비해 혈청 속 LDL-콜레스테롤과 중성지방을 감소시켰다. 피노피브레이트 단독처리에 비해 피노피브레이트와 17베타-에스트라다이올 동시처리는 혈청 속 중성지방에 대해 유익한 변화를 나타내었다. 따라서 본 연구는 저지방식이를 섭취한 난소절제 암컷 쥐에서 fenofibtae 단독처리에 의해 감소된 혈청 속 중성지방은 피노피브레이트와 17베타-에스트라다이올의 동시처리에 의해 개선될 수 있음을 발견하였다.

Keywords

References

  1. K. Schoonjans, B. Staels, J. J. Auwerx, "Role of the peroxisome proliferator-activated receptor (PPAR) in mediating the effects of fibrates and fatty acids on gene expression", Journal of lipid research, Vol.37, No.5 pp. 907-925, (1996). https://doi.org/10.1016/S0022-2275(20)42003-6
  2. S. A. Kliewer, J. M. Lehmann, T. M. Wilson, "Orphan nuclear receptors: shifting endocrinology into reverse", Science, Vol.284, No.5415 pp. 757-776, (1999). https://doi.org/10.1126/science.284.5415.757
  3. R. Hertz, J. Bishara-Shieban, J. Bar-Tana, "Mode of action of peroxisome proliferators as hypolipidemic drugs. Suppression of apolipoprotein C-III", The Journal of biological chemistry, Vol.270, No.22 pp. 13470-13475, (1995). https://doi.org/10.1074/jbc.270.22.13470
  4. T. Osumi, S. Osada, T. Tsukamoto, "Analysis of peroxisome proliferator-responsive enhancer of the rat acyl-CoA oxidase gene", Annals of the New York Academy of Sciences, Vol.804, pp. 202-213, (1996). https://doi.org/10.1111/j.1749-6632.1996.tb18617.x
  5. K. Schoonjans, J. Peinado-Onsurbe, A. M. Lefebvre, R. A. Heyman, M. Briggs, S. Deeb, B. Staels, J. Auwerx, "PPARalpha and PPARgamma activators direct a distinct tissue-specific transcriptional response via a PPRE in the lipoprotein lipase gene", The EMBO Journal, Vol.15, No.19 pp. 5336-5348, (1996). https://doi.org/10.1002/j.1460-2075.1996.tb00918.x
  6. M. Pawlak, P. Lefebvre, B. Staels B, "Molecular mechanism of PPAR${\alpha}$ action and its impact on lipid metabolism, imflammation and fibrosis in non-alcoholic fatty liver disease", Journal of Hepatology, Vol.62, No.3 pp. 703-712, (2015).
  7. M. Yoon, S. Jeong, H. Lee, M. Han, J. H. Kang, E. Y. Kim, M. Kim, G. T. Oh, "Fenofibrate improves lipid metabolism and obesity in ovariectomized LDL receptor-null mice", Biochemical and biophysical research communications, Vol.302, No.1, pp. 29-34, (2003). https://doi.org/10.1016/S0006-291X(03)00088-3
  8. E. Chaput, R. Saladin, M. Silvestre, A. D. Edgar, "Fenofibrate and rosiglitazone lower serum triglycerides with opposing effects on body weight", Biochemical and biophysical research communications, Vol.271, No.2 pp. 445-450, (2000). https://doi.org/10.1006/bbrc.2000.2647
  9. M. Carr, "The Emergence of the Metabolic Syndrome with Menopause", The Journal of Clinical Endocrinology and Metabolism, Vol.88, No.6, pp. 2404-2411, (2003). https://doi.org/10.1210/jc.2003-030242
  10. M. Abbey, A. Owen, M. Suzakawa, P. Roach, P. J. Nestel, "Effects of Menopause and Hormone Replacement Therapy on Plasma Lipids, Lipoproteins and LDL-receptor Activity", Maturitas, Vol.33, No.3 pp. 259-269, (1999). https://doi.org/10.1016/S0378-5122(99)00054-7
  11. C. J. McAloon, L. M. Boylan, T. Hamborg, N. Stallard, F. Osman, P. B. Lim, S. A. Hayat, "The changing face of cardiovascular disease 2000-2012: An analysis of the world health organisation global health estimates data", International Journal of Cardiology, Vol.224, pp. 256-264, (2016). https://doi.org/10.1016/j.ijcard.2016.09.026
  12. R. A. Lobo, "Benefits and Risks of estrogen replacement therapy", American Journal of Obsterics and Gynecology, Vol.173, No.3 pp. 982-989, (1995). https://doi.org/10.1016/0002-9378(95)90247-3
  13. S. Jeong, M. Yoon, "Inhibition of the actions of peroxisome proliferator-activated receptor ${\alpha}$ on obesity by estrogen", Obesity (Silver Spring), Vol.15, No.6 pp. 1430-1440, (2007). https://doi.org/10.1038/oby.2007.171
  14. S. E. Campbell, K. A. Mehan, R. J. Tunstall, M. A. Febbraio, D. Cameron-Smith, "17beta-estradiol upregulates the expression of peroxisome proliferator-activated receptor alpha and lipid oxidative genes in skeletal muscle", Journal of molecular endocrinology, Vol.31, No.1 pp. 37-45, (2003). https://doi.org/10.1677/jme.0.0310037
  15. D. B. Han, W. A. Payne, E. B. Mauer, Focus on Health (7th ed.), pp. 277-288, Mcgraw-Hill Higher Education, (2005).
  16. S. Jeong, "Anti-obesity Effects of Genisterin in Female Ovariectomy-induced Obese Mice", Journal of the Korean Applied Science and Technology, Vol.34, No.3 pp. 34-46, (2017).
  17. M. Yoon, S. Jeong, C. J. Nicol, H. Lee, M. Han, J. J. Kim, Y. J. Seo, C. Ryu, G. T. Oh, "Fenofibrate regulates obesity and lipid metabolism with sexual dimorphism", Experimental and molecular medicine, Vol.34, No.6 pp. 481-488, (2002). https://doi.org/10.1038/emm.2002.67
  18. S. Jeong, "The Effect of Swimming plus Hypolipidemic Agent on Obesity", Journal of the Korean Applied Science and Technology, Vol.36, No.1 pp. 34-46, (2019). https://doi.org/10.12925/JKOCS.2019.36.1.34
  19. L. Ning, Y. Liu, W. Wang, Y. Li, L. Chen, Z. Y. Du, "The metabolic regulation of fenofibrate is dependent on dietary protein content in male juveniles of Nile tilapia (Oreochromis niloticus)", The British journal of nutrition, Vol.122, No.6 pp. 648-656, (2019). https://doi.org/10.1017/S0007114519001594
  20. S. Jeong, M. Han, H. Lee, M. Kim, J. Kim, C. J. Nicol, B. H. Kim, J. H. Choi, K-H Nam, G. T. Oh, M. Yoon, "Effects of fenofibrate on high-fat diet-induced body weight gain and adiposity in female C57BL/6J mice", Metabolism Vol.53, No.10 pp. 1284-1289, (2004). https://doi.org/10.1016/j.metabol.2004.05.003
  21. K. Toda, K. Takeda, S. Akira, T. Saibara, T. Okeda, S. Onishi, Y. Shizuta, "Alterations in hepatic expression of fatty-acid metabolizing enzymes in ArKO mice and their reversal by the treatment with 17 beta-estradiol or a peroxisome proliferator", The Journal of steroid biochemistry and molecular biology, Vol.79, No.1-5 pp. 11-17, (2001). https://doi.org/10.1016/S0960-0760(01)00135-2
  22. R. Okazaki, D. Inoue, M. Shibata, M. Saika, S. Kido, H. Ooka, H. Tomiyama, Y. Sakamoto, T. Matsumoto, "Estrogen promotes early osteoblast differentiation and inhibits adipocyte differentiation in mouse bone marrow stromal cell lines that express estrogen receptor (ER) ${\alpha}$ or ${\beta}$", Endocrinology, Vol.143, No.6, pp. 2349-2356, (2002). https://doi.org/10.1210/endo.143.6.8854