The Journal of the Korea Contents Association (한국콘텐츠학회논문지)

The Korea Contents Association (한국콘텐츠학회)
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Bioinformatic Analysis of Envelope Protein Domains of Zika Virus and Dengue Virus

지카 바이러스 및 뎅기 바이러스의 외피 단백질을 구성하는 도메인의 생물정보학적 분석

  • Received : 2019.10.23
  • Accepted : 2019.11.04
  • Published : 2019.11.28
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Abstract

In recent years, large scale damages from arbovirus infections by mosquitoes have been reported worldwide due to factors such as change in global climate, increased overseas travel, and increased logistics movement between countries. Among them, Zika virus and dengue virus belonging to genus Flavivirus are representative. In this study, we performed in-depth analyses of the envelope (E) protein that perform essential functions for host infection of Zika virus and dengue virus based on bioinformatics databases. The domain analysis of E protein was performed to determine the type, location, and function, and homology analysis for each domain. From these results, EDIII showing low homology was identified. The homology and immunogenicity of each peptide constituting EDIII were analyzed and three-dimensional structures were modeled. Furthermore, we discussed their biological meaning and how they could be used.

최근 지구 기후의 변화, 해외 여행객의 증가 및 국가 간 물류 이동의 증가 등과 같은 요인으로 인해 모기와 같은 절지동물이 매개하는 아보바이러스(arthropod-borne virus, arbovirus) 감염으로 인한 대규모의 피해가 전 세계적으로 끊임없이 발생하고 있다. 그 중에서도 플라비바이러스 속에 해당하는 지카 바이러스와 뎅기바이러스에 의한 피해가 대표적이다. 본 연구에서는 다양한 생물정보학 데이터베이스를 바탕으로 지카 바이러스 및 뎅기 바이러스가 숙주 감염에 필수적인 기능을 수행하는 외피 단백질에 대한 심층적인 분석을 수행했다. 외피 단백질을 구성하는 도메인들에 대한 분석을 통해 도메인의 종류, 위치 및 기능을 파악했으며 각 도메인별 상동성을 분석했다. 이로부터 낮은 상동성을 보이는 도메인인 EDIII를 도출하였으며, EDIII를 구성하는 펩타이드에 대한 상동성 및 면역원성 분석과 3차원 구조 모델링을 수행했다. 더 나아가 이들이 갖는 생물학적 의미와 활용 방안에 대해 논의했다.

Keywords

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