한방안이비인후피부과학회지 (The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology)

  • 제31권4호
  • /
  • Pages.1-12
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  • 2018
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  • 1738-6640(pISSN)
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  • 2234-4020(eISSN)
대한한방안이비인후피부과학회 (The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology)
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창출금련탕(蒼朮芩連湯) 추출물의 항산화 및 항염증 활성에 미치는 영향

The anti-oxidant and anti-inflammatory Activites of Changchulgeumryeontang Extract

  • 원혜련 (세명대학교 한방화장품과학과) ;
  • 박혜수 (세명대학교 한의과대학 안이비인후피부과학교실) ;
  • 김이화 (세명대학교 한의과대학 경락경혈학교실) ;
  • 김용민 (세명대학교 한방화장품과학과)
  • Won, Hea-Ryeon (Dept. of Oriental Cosmetic Sciences, Semyung University) ;
  • Park, Hye-Su (Dept. of Oriental Ophthalmology, Otolaryngology & Dermatology, College of Korean Medicine, Semyung University) ;
  • Kim, Ee-Hwa (Dept. of Meridian and Acupoint, College of Korean Medicine, Semyung University) ;
  • Kim, Yong-Min (Dept. of Oriental Cosmetic Sciences, Semyung University)
  • 투고 : 2018.10.09
  • 심사 : 2018.11.12
  • 발행 : 2018.11.25
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초록

Objectives : This study investigated the anti-oxidant and anti-inflammatory activities of Changchulgeumryeontang (CCGRT) extract. Methods : The macrophage cell line RAW 264.7 cells were used and MTT assay was performed to measure the cell viabilities at the various concentrations of CCGRT ($25-200{\mu}g/m{\ell}$). Nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$) were measured in LPS-induced RAW 264.7 cells. Expressions of iNOS, $NF-{\kappa}B$, $IL-1{\alpha}$, $IL-1{\beta}$ and IL-6 were also performed by real-time PCR. The anti-oxidant activities of CCGRT was measured by DPPH radical scavenging activity. Results : 1. there was no cytotoxicity in RAW264.7 cells treated with CCGRT compared to the control. 2. CCGRT treated group significantly inhibited NO and $PGE_2$ production compared to the LPS treated group. 3. CCGRT treated group significantly decreased mRNA expressions of iNOS, $NF-{\kappa}B$, $IL-1{\alpha}$, $IL-1{\beta}$ and IL-6 compared to the LPS treated group. 4. CCGTR was found to have high DPPH free radical scavenging ability. Conclusions : According to the above results, CCGRT may be a potentional choice for the treatment of inflammatory skin disease. Conclusions : According to the above results, CCGRT may be a potentional choice for the treatment of inflammatory skin disease.

키워드

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