Journal of the Korean Society of Radiology (한국방사선학회논문지)

The Korean Society of Radiology (한국방사선학회)
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Hepatic Lipid Changes in Fatty Liver Rat Model by In Vivo Short-TE 1H-MRS

생체 내 양성자 자기공명분광법을 이용한 지방간 쥐에서 간 지질 변화

  • Kim, Joo-Yeon (Department of Biomedical Engineering, Chungbuk University) ;
  • Baek, Hyeon-Man (Department of Molecular Medicine, Lee Gil Ya Cancer & Diabetes Institute, Gachon University)
  • 김주연 (충북대학교 의공학과) ;
  • 백현만 (가천대학교 융합의과대학.이길여 암당뇨연구원 분자의학과)
  • Received : 2018.09.23
  • Accepted : 2018.10.31
  • Published : 2018.10.31
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Abstract

Non-alcoholic fatty liver disease is the most common cause of chronic liver diseases. This study was to characterize early hepatic lipid changes in fatty liver rat model by in vivo short-echo time(TE) $^1H$-MRS(Proton - Magnetic Resonance Spectroscopy). Each the examinations were measured from liver parenchyma in rats at 0, 2, 4, 6, 8 weeks followed by high fat diet, respectively. Significant increase in lipid signals. 0.9, 1.3, 2.3, 2.8, and 5.3 ppm was found in animals with 2 weeks(p<0.01). Therefore, $^1H$-MRS is useful in detecting and characterizing various hepatic lipid alterations as early as 2 weeks for the start high fat diet.

비알코올성 지방간 질환은 만성 간질환의 가장 흔한 원인이다. 이 연구의 목적은 비알코올성 지방간 질환 동물모델을 확립하고 생체 내 짧은 양성자 자기공명분광법을 이용하여 비침습적으로 간의 대사물질과 지질의 변화를 정량 분석하고자 하였다. 각 실험은 고지방식 먹이를 먹고 나서 0주를 기준선으로 하고, 2, 4, 6, 8주에 쥐의 간 실질 조직으로부터 측정되었다. 0주를 기준선으로 하고 2주와 비교했을 때 0.9, 1.3, 2.3, 2.8 및 5.3 ppm 에서 유의미하게 증가하였다(p<0.01). 따라서 양성자 자기공명분광법은 고지방식 먹이를 먹고 2주 후부터 다양한 쥐의 간 지질 변화를 검출하고 특성화하는데 유용할 것이다.

Keywords

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