Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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fects of Cuscuta Chinensis Lamark Ethanol Extract on Wrinkle Improvement Bio-markers by UVB-induced CCD-986Sk Cell

토사자 에탄올 추출물이 UVB로 유도된 CCD-986Sk cell에서 주름개선 생리지표에 미치는 영향

  • Joo, In Hwan (Department of Pathology, College of Korean Medicine, Daejeon University) ;
  • Choi, Hak Joo (Traditional and Biomedical Research Center(TBRC-RIC), Daejeon University) ;
  • Sim, Boo Yong (Traditional and Biomedical Research Center(TBRC-RIC), Daejeon University) ;
  • Min, Ga Yul (Department of Pathology, College of Korean Medicine, Daejeon University) ;
  • Kim, Dong Hee (Department of Pathology, College of Korean Medicine, Daejeon University)
  • 주인환 (대전대학교 한의과대학 병리학교실) ;
  • 최학주 (대전대학교 난치성 면역질환의 동서생명의학연구 지역혁신센터) ;
  • 심부용 (대전대학교 난치성 면역질환의 동서생명의학연구 지역혁신센터) ;
  • 민가율 (대전대학교 한의과대학 병리학교실) ;
  • 김동희 (대전대학교 한의과대학 병리학교실)
  • Received : 2018.07.10
  • Accepted : 2018.09.11
  • Published : 2018.10.25
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Abstract

The purpose of this study was to investigate the effects of Cuscuta chinensis Lamark ethanol extract (CL) on wrinkle improvement. Cuscuta chinensis Lamark is known to contain dried saccharide, alkaloids, flavonoids, lignans and rein glycoside as major components of dried mature seeds of Cuscuta japonica Choisy. In this study, we evaluated the anti-wrinkle effects of CL and investigated bio-markers (e.g ; MMP-1, MMP-3, MMP-9, TIMP-1, type I procollagen) associated with skin wrinkle improvement. We tested the anti-wrinkle effect of CL using human fibroblast called CCD-986Sk cell. We observed an increase in MMPs, TIMP-1, and type 1 pro-collagen CL in CCD-986Sk cells irradiated with UVB at an intensity of $2mJ/cm^2$ for 60 seconds. As a result, CL decreased UVB-induced MMPs levels and mRNA expressions in CCD-986Sk cell. The levels and mRNA expressions of type I procollagen and TIMP-1 were increased by CL. These results suggest that CL has activities on improvement of skin wrinkle, which is induced by UVB radiation. Taken together, this study proposed the possibility of developing herbal medicine and functional herbal cosmetic materials with wrinkle-improving effects of Cuscuta chinensis Lamark.

Keywords

References

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