Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Anti-microbial Activities of Ten Lauraceae Species against Propionibacterium acnes

여드름 유발균 Propionibacterium acnes에 대한 녹나무과 10종의 항균활성

  • Cho, Ju Sung (Quality Control & Seed Tech Team, Nongwoo Bio CO., LTD.) ;
  • Chi, Lai Won (Major in Horticulture, Division of Animal, Horticultural, and Food Sciences, Chungbuk National University) ;
  • Jang, Bo Kook (Major in Horticulture, Division of Animal, Horticultural, and Food Sciences, Chungbuk National University) ;
  • Jeong, Heon Sang (Department of Food Science and Biotechnology, Chungbuk National University) ;
  • Lee, Cheol Hee (Major in Horticulture, Division of Animal, Horticultural, and Food Sciences, Chungbuk National University)
  • Received : 2018.04.26
  • Accepted : 2018.06.05
  • Published : 2018.10.31
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Abstract

This study was performed to develop a new natural antimicrobial materials by analyzing the effect of extracts obtained from Ten Lauraceae Species on the inhibitory activity against Propionibacterium acnes. The plant materials were collected from Wando and Jeju islands, and the antimicrobial activity of the crude extracts was examined by the agar diffusion method with different part (i.e., leaf and branch), solvents (i.e., distilled water, 80% ethanol, and 100% methanol) and at different ultrasonic extracting times (i.e., 15, 30, and 45 minutes). The control agents used were synthetic antimicrobials, methylparaben and phenoxyethanol, at concentrations of 0.4, 1, 2, and 4 mg/disc. Altogether, extracts of 10 species used in the study showed inhibitory activity, which confirmed their antimicrobial action against acnes. Among these, leaves of Laurus nobilis L. which was extracted in 80% ethanol for 45 min showed the largest clear zone (19.8 mm). Leaves of L. nobilis L., showing highest antimicrobial activities among 10 species, were successively reextracted with n-hexane, chloroform, ethylacetate and n-butanol. As a results, in all fractions except butanol, clear zone above 10 mm were formed. The ethyl acetate fraction showed the highest inhibitory activity (13.3 mm) and the inhibitory activity was significantly higher than that of crude extract (10.2 mm) and phenoxyethanol as a control (12.5 mm).

본 연구는 녹나무과 10종의 추출물이 Propionibacterium acnes의 억제활성에 미치는 영향을 분석하여 새로운 천연 항균소재를 개발하기위해 수행되었다. 실험재료는 완도와 제주도에서 채집하였으며, 부위(잎, 가지), 용매(증류수, 80% 에탄올, 100% 메탄올) 및 초음파 추출시간(15, 30, 45분)에 따른 조추출물의 항균활성을 agar diffusion method로 조사하였다. 대조구로는 합성항균제인 methylparaben과 phenoxyethanol 0.4, 1, 2, 4 mg/disc를 사용하였다. 연구에 사용된 10종의 모든 추출물에서 억제활성을 보였으며, 여드름균에 대한 항균작용을 확인할 수 있었다. 이 중 월계수는 80% 에탄올에 45분간 추출한 조건에서 20.2 mm의 생육억제환을 나타내어 가장 우수한 여드름균에 대한 항균작용을 확인할 수 있었다. 월계수 잎을 100% 메탄올을 용매로 초음파 30분 추출한 다음n-hexane, chloroform, ethyl acetate, n-butanol 그리고 water 순으로 용매 분획하여 항균활성을 비교분석하였다. 결과, butanol 분획물을 제외한 모든 분획물에서 10.0 mm 이상의 억제환이 형성되었다. 그중 ethyl acetate 분획물에서 억제환이 13.3 mm로 가장 컸으며, 조 추출물(10.2 mm)과 대조구인 phenoxyethanol (12.5 mm)에 비해서도 유의적으로 우수한 억제활성을 나타내었다.

Keywords

References

  1. Ahn, B.K., E.H. Choi and S. Lee. 2002. The pathogenesis of acne. J.S.B.R. 4:62-70.
  2. Bae, H.G. and S.P. Moon. 2001. Structure of evergreen broad-leaved forest community at Baegunbong area in Wando. Forest Sci. Technol. 90:756-766.
  3. Brown, S.K. and A.R. Shalita. 1998. Acne vulgaris. The Lancet 351:1871-1876. https://doi.org/10.1016/S0140-6736(98)01046-0
  4. Choi, S.M., M.J. Kim, Y.H. Choi, H.J. Ahn and Y.P. Yun. 1998. Screening of the antibacterial activity of natural products against Propionibacterium acnes. Yakhak Hoeji 42:89-94.
  5. Choi, Y.G., Y.S. Kim, G.S. Cho and C.K. Sung. 2002. Screening for antimicrobial activity from Korean plants. J. Korean Soc. Food Nutr. 15:300-306.
  6. Eady, E.A., M. Gloor and J.J. Leyden. 2003 Propionibacterium acnes resistance a worldwide problem. Dermatology 206:54-56. https://doi.org/10.1159/000067822
  7. Foran, C.M., E.R. Bennett and W.H. Bebson. 2000. Developmental evaluation of a potential non-steroidal estrogen: triclosan. Mar. Environ. Res. 50:153-156. https://doi.org/10.1016/S0141-1136(00)00080-5
  8. Ha, Y.M., B.B. Lee, H.J. Bae, K.M. Je, S.R. Kim, J.S. Choi and I.S. Choi. 2009. Anti-microbial activity of grapefruit seed extract and processed sulfur solution against human skin pathogen. J. Life Sci. 19:94-100. https://doi.org/10.5352/JLS.2009.19.1.094
  9. Jeon, M.A., H.J. Kwon, Y.H. Kim, K.I. Han, H.N. Chung, S.K. Chung, I.S. Bang and M.D. Han. 2010. Antioxidative activities and antiproliferation effects on oral carcinoma KB cell of the brazilin from Caesalpinia sappan L. J. Dent. Hyg. Sci. 10:365-372.
  10. Jung, H. 2010. Antimicrobial activity of 4 rheum plants against methicillin-resistant Staphylococcus aureus. Department of Oriental Medicine Resources, MS Thesis. Sunchon National University, South Korea.
  11. Kang, M.H., M.K. Oh, J.K. Bang, D.H. Kim, C.H. Kang and B.H. Lee. 2002. Varietal difference of lignan contents and fatty acids composition in Korean sesame cultivars. Korean J. Crop Sci. 45:203-206.
  12. Kim, E.I., B.M. Jung and A.J. Wi. 2007. A study on the physiological and psychological effects of volatile substances from Cinnamomum camphora and C. japonicum. J. People Plants Environ. 10:79-86.
  13. Kim, H.S. and H.J. Byeom. 2007. Beauty science I. Chung-ku Publisher, Seoul, South Korea.
  14. Kim, J.M., H.C. Oh, S.P. Sing, M.K. Kim and C.Y. Hwang. 2006. Study on the anti-microbacterial activity, anti-inflammatory and anti-allergic effects of several herb-extract. Korean J. Orient. Physiol. Pathol. 20:103-114.
  15. Kim, J.Y., W.J. Yoon, E.Y. Yim, S.Y. Park, Y.J. Kim and G. Song. 2011. Antioxidative and antimicrobial activities of Castanopsis cuspidata var. sieboldii extracts. Korean J. Plant Res. 24:200-207. https://doi.org/10.7732/kjpr.2011.24.2.200
  16. Kim, K.J., S.I. Jeong, J.D. Cha, S.M. Kim and Y.O. You. 2004. Inhibitory effects of Caesalpinia sappan on growth and invasion of methicillin-resistant Staphylococcus aureus. J. Ethnopharmacol. 91:81-87. https://doi.org/10.1016/j.jep.2003.11.017
  17. Kim, N.R., H.J. Kwon, J.S. Cho and C.H. Lee. 2012. Antioxidant activities of fractions obtained from Dryopteris crassirhizoma, D. nipponensis and Polystichum lepidocaulon. Korean J. Plant Res. 25:176-183. https://doi.org/10.7732/kjpr.2012.25.2.176
  18. Kim, S.I. and Y.S. Han. 1997. Isolation and identification of antimicrobial compound from sancho (Zanthoxylum Schinifolium). Korean J. Soc. Food. Sci. 13:56-63.
  19. Kim, S.S. 2008. Antimicrobial properties of extracts form Ostericum koreanum extracts on acnes strains. Department of Beauty Business, MS Thesis. Soogsil University, South Korea.
  20. Kim, Y.S., H.S. Park, K.S. Kim, H.S. Shin, B.S. Kim, W.S. Choi, S.W. Lee and T.J. Park. 1995. Postmarketting surveillance for nonsteroidal antiinflammatory drugs. J. Korean Acad. Fam. Med. 16:600-607.
  21. Kivcakb, B. and T. Mert. 2002. Preliminary evaluation of cytotoxic properties of Laurus nobilis extracts. Fitoterapia 73:242-243. https://doi.org/10.1016/S0367-326X(02)00060-6
  22. Korea Biodiversity Information System. 2018. http://www.nature.go.kr/kbi/plant/pilbk/selectPlantPilbkDtl.do (accessed 04 2018)
  23. Lambert, R.J.W., P.N. Skandamis, P.J. Coote and G.H.E. Nychas. 2011. A study of the minimum inhibitory concentration and mode of action of oregano essential oil, thymol and carvacrol. J. Appl. Microbiol. 91:453-562.
  24. Lee, H.S. and J.A. Yoon. 2010. Inhibitory activity of advanced glycation endproducts (AGE) formation of edible plants for development of anti-wrinkle ingredients. J. Korean Soc. Food Sci. Nutr. 39:186-192. https://doi.org/10.3746/jkfn.2010.39.2.186
  25. Lee, O.H., H.B. Lee, J.S. Lee, J.Y. Son, S.K. Rhee, H.D. Kim, Y.C. Kim and B.Y. Lee. 2005. Chemical properties of olive and bay leaves. J. Korean Soc. Food Sci. Nutr. 34:503-508. https://doi.org/10.3746/jkfn.2005.34.4.503
  26. Lee, R. and M.J. Balick. 2005. Sweet wood-cinnamon and its importance as a spice and medicine. J. Sci. Healing 1:61-64.
  27. Lee, S.H., S.J. Kim, J.R. Park, E.H. Jo, N.S. Ahn, J.S. Park, J.W. Hwang, J.Y. Jung, Y.S. Lee and K.S. Kang. 2006. Oestrogenic activity of parabens in vitro estrogen assays. J. Fd. Hyg. Safety 21:100-106.
  28. Mo, J.H. and S.J. Oh. 2013. Tyrosinase inhibitory activity and melanin production inhibitory activity of the methanol extract and fractions from Dendropanax morbifera lev. Asian J. Beauty Cosmetol. 11:275-280.
  29. Otvos, L.J.R. 2000. Antibacterial peptide from insects. J. Pept. Sci. 6:497-511. https://doi.org/10.1002/1099-1387(200010)6:10<497::AID-PSC277>3.0.CO;2-W
  30. Paniwnyk, L., E. Beaufoy, J.P. Lorimer and T.J. Mason. 2001. The extraction of rutin from flower buds of Sophora japonica. Ultrason. Sonochem. 8:299-301. https://doi.org/10.1016/S1350-4177(00)00075-4
  31. Park, C.S. 1998. Antibacterial activity of ethanol extract of pine needle against pathogenic bacteria. Korean J. Postharvest Sci. Technol. 5:380-385.
  32. Park, S.H., K.B.W.R. Kim, M.J. Kim, J.S. Choi, Y.J. Cho and D.H. Ahn. 2017. Antimicrobial activity of extracts from different parts and essential oil from Pinus densiflora on skin pathogens. J. Life Sci. 6:646-651.
  33. Park, S.J., Y.H Rhu, S.G Bae and D.G. Ahn. 2017. Screening of antifungal activities of plant extracts against phytopathogenic fungi. Korean J. Plant Res. 30:343-351.
  34. Park, U.Y., D.S. Chang and H.R. Cho. 1992. Screening of antimicrobial activity for medicinal herbs extracts. J. Korean Soc. Food Sci. Nutr. 21:91-96.
  35. Rural Development Administration. 2007. Utilization technology of tree essential oils as antimicrobial, antifungal, and functional hair growth materials. Rural Development Administration, Seoul, South Korea.
  36. Simic, M. 2003. Preliminary assay on the antioxidative activity of Laurus nobilis extract. Fitoterapia 74:613-616. https://doi.org/10.1016/S0367-326X(03)00143-6
  37. Son, D.B., S.P. Song, C.H. Hwang, S.H. Hong and C.Y. Hwang. 2005. Study on the anti-microbial activity, anti-inflammatory and anti-alergic effects of mixture of Rheum coreanum, Scutellaria baikalensis, Phellodenron amurense. J. Korean Orient. Med. Ophtalmol. Otolaryngol. Dermatol. 18:154-171.
  38. Suh, D.H. 2010. Pharmacologic treatment of acne. J. Korean Med. Assoc. 53:623-629. https://doi.org/10.5124/jkma.2010.53.7.623
  39. Suk, K.D., Lee S.H. and Kim K.S. 2004. Growth-inhibitory effects of Cuscuta japonica Choisy's and C. australis R.Be's extract against Propionibacterium acnes. Korean J. Pharmacogn. 35:375-379.
  40. Yang, H.J., M.j. Park and H.S. Lee. 2011. Antioxidative activities and components of Gardenia jasminoides. Korean J. Food Sci. Technol. 43:51-57. https://doi.org/10.9721/KJFST.2011.43.1.051
  41. Yu, J.M. and H.I. Moon. 2018. Antioxidants and acetyl-cholinesterase inhibitory activity of solvent fractions extracts from Dendropanax morbiferus. Korean J. Plant Res. 31:10-15.