생명과학회지 (Journal of Life Science)

  • 제28권10호
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  • Pages.1156-1162
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  • 2018
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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다양한 부위의 연(Lotus) 열수 추출물의 항혈전 활성

Anti-thrombotic Activities of Hot-water Extracts Prepared from Various Parts of Nelumbo nucifera Gaertner

  • Ahn, Seon-Mi (Department of Food and Nutrition, Andong National University) ;
  • Sung, Hwa-Jung (Department of Food and Nutrition, Andong National University) ;
  • Kim, Jong-Sik (Department of Biological Science, Andong National University) ;
  • Park, Jong-Yi (Gyeongbuk Institute For Bio-Industry) ;
  • Sohn, Ho-Yong (Department of Food and Nutrition, Andong National University)
  • 투고 : 2018.05.23
  • 심사 : 2018.08.08
  • 발행 : 2018.10.30
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초록

연(Lotus)의 항혈전 활성을 평가하기 위해, 연잎, 연자방, 연자육, 연자심, 연근 및 우절로부터 각각 열수 추출물을 제조하고, 이의 성분 분석, 항응고, 혈소판 응집저해 및 적혈구 용혈활성을 평가하였다. 연의 부위별 추출효율은 우절>연자심>연근>연잎>연자육>연자방의 순으로 나타났으며, 추출물들의 pH는 5.6~6.5, 산도는 0.06~0.20%로 다양하게 나타났다. Brix의 경우 연자육에서 70%로 가장 높게 나타난 반면, 연잎과 연자방에서는 분석한계(0.1%) 이하로 나타났다. TP 및 TF 함량은 연잎에서 179.7 및 68.5 mg/g으로 가장 높은 함량을 나타내었고, 총당은 연자육에서 873.0 mg/g, 환원당은 연잎에서 161.4 mg/g으로 가장 높게 나타났다. 한편 항응고 활성은 우절, 연잎 및 연자방 추출물에서 우수하였으며, 특히 연자방의 경우 TT, PT 및 aPTT 평가에서 무처리구에 비해 모두 15배 이상 연장된 강력한 활성을 나타내었다. 그러나, 혈소판 응집 저해 활성을 평가한 결과 연자심 추출물에서만 0.25 mg/ml 농도에서 무첨가구에 비해 25.1%의 혈소판 응집을 나타내어 강력한 응집저해 활성을 나타내었다. 인간 적혈구에 대한 용혈활성 평가결과, 연자육 추출물에서만 6.1%의 약한 용혈활성을 나타내었고, 나머지 부위 추출물에서는 용혈활성이 나타나지 않았다. 본 연구결과는 연 산업의 부산물인 우절, 연자방, 연자육 및 연자심이 혈전성 질환 예방 및 치료용 식의약품 소재로 개발 가능함을 제시하고 있다.

To investigate the anti-thrombotic activities of the lotus (Nelumbo nucifera Gaertner), various hot-water extracts were prepared from the leaf, pod of seed (PS), seed, embryo of seed (ES), root, and the node of root (NR) of the lotus. The highest extraction ratio was found in the NR (20.3%), followed by the seed, root, leaf, ES, and PS. These extracts had pH and acidity levels ranging from 5.6~6.5 and 0.06~0.20%, respectively. The seed extract showed 70% brix, whereas the leaf and PS extracts showed less than 0.1% brix. The highest contents of total polyphenol (179.7 mg/g), total flavonoids (161.4 mg/g), and reducing sugar (161.4 mg/g) were observed in the leaf extract, and the highest total sugar content (873.0 mg/g) in the seed extract. Anti-coagulation assay of the extracts of NR, leaf, and PS showed strong activities. In particular, at a concentration of 5 mg/ml, the PS extract had 15-fold extended thrombin, prothrombin, and activated partial thromboplastin times. However, only the ES extract showed activities inhibitory to platelet aggregation, with treatment with 0.25 mg/ml of ES extract decreasing platelet aggregation to 25.1%, a reduction comparable to that effected by aspirin. The extracts other than the seed extract showed no hemolysis activities against human RBC at treatments of up to 1 mg/ml. These results suggest that the NR, PS, seed, and ES, all byproducts of the lotus agriculture industry, have high potential as novel sources of anti-thrombotic agent.

키워드

참고문헌

  1. Asokan, S. M., Maraippan, R., Muthusamy, S. and Velmurugan, B. K. 2018. Pharmacological benefits of neferine - A comprehensive review. Life Sci. 199, 60-70. https://doi.org/10.1016/j.lfs.2018.02.032
  2. Bijak, M., Bobrowski, M., Borowiecka, M. and Podsedek, A. 2011. Anticoagulant effect of polyphenols-rich extracts from black chokeberry and grape seeds. Fitoterapia 82, 811-817. https://doi.org/10.1016/j.fitote.2011.04.017
  3. Chang, M. S., Kim, H. M., Yang, W. M., Kim, D. R., Park, E. H., Ko, E. B., Choi, M. J., Kim, H. Y., Oh, J. H., Shim, K. J., Yoon, J. W. and Park, S. K. 2007. Inhibitory effects of Nelumbo nucifera on tyrosinase activity and melanogenesis in Clone M-3 melanocyte cells. Kor. J. Herbology 22, 87-94.
  4. Chen, H., Qi, X., He, C., Yin, Z., Fan, D. and Han, G. 2013.Coagulation imbalance may not contribute to the development of portal vein thrombosis in patients with cirrhosis. Thromb. Res. 131, 173-177. https://doi.org/10.1016/j.thromres.2012.11.003
  5. Eikelboom, J. W., Zelenkofske, S. L. and Rusconi, C. P. 2010. Coagulation factor IXa as a target for treatment and prophylaxis of venous thromboembolism. Arterioscler. Thromb. Vasc. Biol. 30, 382-387. https://doi.org/10.1161/ATVBAHA.110.203117
  6. Huh, M. K. and Kim, H. J. 2014. Antibacterial effect on leafextract from Nelumbo nucifera against oral microorganism. J. Kor. Soc. Dent. Hyg. 14, 117-122.
  7. Hwang, H. J., Kang, M. S., Kim, B. K., Jung, B. M. and Kim, M. H. 2012. The effect of Opuntia humifusa seed extracts on platelet aggregation and serum lipid level in ovariectomized rats. J. Life Sci. 22, 1680-1687.
  8. Jeong, S. H., Kim, S. I. and Sim, K. H. 2012. Antioxidative activity of jeolpyeon containing lotus (Nelumbo nucifera Gaertn) seed powder. Kor. J. Food Culture 27, 505-511. https://doi.org/10.7318/KJFC/2012.27.5.505
  9. Jung, I. C. and Sohn, H. Y. 2014. Antioxidation, antimmicorbial and antithrombosis activities of aged black garlic (Allium sativum L.). Kor. J. Microbiol. Biotechnol. 42, 285-292. https://doi.org/10.4014/kjmb.1407.07002
  10. Kim, M. D., Rhee, S. K. and Seo, J. H. 2001. Enhanced production of anticoagulant hirudin in recombinant Saccharomyces cerevisiae by chromosomal ${\delta}$-integration. J. Biotechnol. 85, 41-48.
  11. Kim, M. S., Oh, I. T, Jun, D. Y., Lee, J. Y., Sohn, H. Y., Kwak, D. Y., Seo, M. C., Woo, K. S., Ko, J. Y., Jung, T. W., Nam, M. H., Woo, M. H. and Kim, Y. H. 2013. Anticoagulant activity and fibrinolytic activities of Hwanggeumchal sorghum in vitro. J. Life Sci. 23, 1460-1470. https://doi.org/10.5352/JLS.2013.23.12.1460
  12. Kim, M. S., Park, Y. J. and Sohn, Y. W. 2010. Antioxidative effect and melanogenesis of Nelumbo nucifera stamen extract on cultured human skin melanoma cells injured by hydrogen peroxide. Kor. J. Plant Res. 23, 145-150.
  13. Kim, M. S. and Sohn, H. Y. 2015. Anti-coagulation and anti- platelet aggregation activity of the mature fruit of Sorbus commixa. Microbiol. Biotechnol. Lett. 43, 373-377.
  14. Kim, O. K. 2014. Ethanol effect of Nelumbo nucifera root on carbohydrate methabolism related enzyme activities and antioxidative effect in streptozotocin-induced diabetic rats. J. Kor. Oil. Chem. Soc. 31, 509-516. https://doi.org/10.12925/jkocs.2014.31.3.509
  15. Kim, Y. G., Kwon, M. J. and Cho, S. I. 2005. Effect of nodus Nelumbinis rhizomatis extract on the regulation of regional cerebral blood flow in rats. Kor. J. Herbology 20, 75-81.
  16. Kim, Y. H., Choi, K. S. and Shin, K. O. 2016. The effect of lotus (Nelumbo nucifera) leaf, stem, and yeonjabang powder extract on the biochemical factors in serum in mice fed a high-fat diet. Kor. J. Food Nutr. 29, 684-691. https://doi.org/10.9799/ksfan.2016.29.5.684
  17. Lee, J. J., Ha, J. O. and Lee, M. Y. 2007. Antioxidaive activity of lotus root (Nelumbo nucifera G.) extracts. J. Life Sci. 17, 1237-1243. https://doi.org/10.5352/JLS.2007.17.9.1237
  18. Lee, J. Y., Im, K. R., Jung, T. K. and Yoon, K. S. 2013. The inhibitory effects of Nelumbo nucifera Gaertner extract on melanogenesis. Kor. Soc. Biotechnol. Bioeng. 28, 137-145.
  19. Lee, K. S., Kim, M. G. and Lee, K. Y. 2006. Antioxidative activity of ethanol extract from lotus (Nelumbo nucifera) leaf. J. Kor. Soc. Food Sci. Nutr. 35, 182-186. https://doi.org/10.3746/jkfn.2006.35.2.182
  20. Lee, K. S. and Lee, K. Y. 2011. Effect of lotus (Nelumbo nucifera) leaf extract on serum and liver lipid levels of rats fed a high fat diet. J. Kor. Soc. Food Sci. Nutr. 40, 1544-1547. https://doi.org/10.3746/jkfn.2011.40.11.1544
  21. Lee, K. W., Kim, Y. H. and Shin, K. O. 2017. In vitro antioxidant activities and antimicrobial activity of lotus (leaf, stem, and seed pod) extracts. Kor. J. Food Nutr. 30, 771-779.
  22. Lee, S. J., Bose, S., Lee, S. J., Jeong, J. E., Koo, B. S., Kim, D. I. and Kim, H. J. 2013. Effects of fermented lotus extracts on the differentiation in 3T3-L1 preadipocytes. J. Kor. Med. Obes. Res. 13, 74-83.
  23. Ling, Z. Q., Xie, B. J. and Yang, E. L. 2005. Isolation, characterization, and determination of antioxidative oligomeric procyanidins from the seedpod of Nelumbo nucifera Gaertn. J. Agric. Food Chem. 53, 2441-2445. https://doi.org/10.1021/jf040325p
  24. Moon, J. H., Hong, K. H. and Yoo, S. S. 2016. Antioxidant properties of the lotus leaf powder content of cheongpomuk. Cul. Sci. Hospital. Res. 22, 112-130. https://doi.org/10.20878/cshr.2016.22.7.010
  25. Park, J. H., Lee, B. G., Byun, G. I. and Kim, D. W. 2010. Antioxidant activities and inhibitory effect on oxidative DNA damage of Nelumbinis Semen extracts. Kor. J. Herbology 25, 55-59.
  26. Park, S. H, Hyun, J. S, Shin, E. H. and Han, J. H. 2005. Functional evaluation of lotus root on serum lipid profile and health improvement. J. East Asian Soc. Dietary Life. 15, 257-263.
  27. Ryu, H. Y., Ahn, S. M., Kim, J. S. and Sohn, H. Y. 2010. Evaluation of in-vitro anticoagulation activity of 33 different medicinal herbs. J. Life Sci. 20, 922-928. https://doi.org/10.5352/JLS.2010.20.6.922
  28. Shin, M. K. and Han, S. H. 2006. Effects of lotus (Nelumbo Nucifera Gaertner) leaf powder on lipid concentrations in rats fed high fat diet rats. Kor. J. Food Culture 21, 202-208.
  29. Singleton, V. L., Orthofer, R. and Lamuela-Raventos, R. M. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocaleau reagent. Methods Enzymol. 299, 152-178.
  30. Sweeney, J. D., Hoerning, L. A., Behrens, A. N., Novak, E. and Swank, R. T. 1990. Thrombocytopenia after desmopressin but absence of in-vitro hypersensitivity to ristocetin. Am. J. Clin. Pathol. 93, 522-525.
  31. Valentina, U., Fabcic, J. and Stampar, F. 2007. Sugars, organic acids, phenolic composition and antioxidant activity of sweet cherry (Prunus avium L.). Food Chem. 107, 185-192.
  32. Yoo, D. H., Joo, D. H., Lee, S. Y. and Lee, J. Y. 2015. Antioxidant effect of Nelumbo nucifera G. leaf extract and inhibition of MITF, TRP-1, TRP-2, and tyrosinase expression in a B16F10 melanoma cell line. J. Life Sci. 25, 1115-1123. https://doi.org/10.5352/JLS.2015.25.10.1115