DOI QR코드

DOI QR Code

반응표면분석법을 이용한 캠벨얼리 포도의 총 안토시아닌과 총 페놀의 초음파 추출조건 최적화

Optimization of ultrasound-assisted extraction of anthocyanins and phenolic compounds from campbell early grape using response surface methodology

  • 류다연 (서울여자대학교 식품응용시스템학부 식품영양학전공) ;
  • 고은미 (서울여자대학교 식품응용시스템학부 식품영양학전공)
  • Ryu, Dayeon (Major of Food & Nutrition, Division of Applied Food System, Seoul Women's University) ;
  • Koh, Eunmi (Major of Food & Nutrition, Division of Applied Food System, Seoul Women's University)
  • 투고 : 2018.08.21
  • 심사 : 2018.10.10
  • 발행 : 2018.10.31

초록

포도의 안토사이아닌과 페놀 화합물의 고효율 추출을 위해 초음파 추출방법을 이용하여 반응표면분석법으로 조건을 최적화하였다. Box-Behnken design을 사용하여 시료-용매 비율, 추출시간, 초음파의 진폭을 독립변수로 하였으며, 총 안토사이아닌과 총 페놀 함량을 최대로 하는 추출조건을 최적화하였다. 총 안토사이아닌 함량은 3가지 독립변수가 유의적인 요인으로 시료-용매 비율이 증가할수록, 추출시간과 진폭은 감소할수록 총 안토사이아닌 함량이 증가하는 경향을 보였으나, 총 페놀 함량은 3가지 독립변수 모두 유의적인 요인이 아닌 것으로 나타났다. 총 안토사이아닌과 총 페놀 함량의 예측 값과 실측 값이 유사하여 본 연구에서 예측된 회귀식을 신뢰할 수 있음을 보여주었다.

In this study, ultrasound-assisted extraction conditions of total anthocyanins and total phenolic compounds from Campbell early grape (Vitis labruscana) were optimized via response surface methodology using a Box-Behnken design. Three independent variables, including solid-liquid ratio (1/30-1/50 g/mL), extraction time (2-10 min), and amplitude (20-100%), significantly affected total anthocyanin content. However, no significant effect of these variables was observed in the total phenolic compound content. The optimized extraction conditions were solid-liquid ratio of 1/48.13 g/mL, extraction time of 2 min, and amplitude of 20%. Under these conditions, the predicted values of total anthocyanins and total phenolic compounds were 94.96 and 1,661.16 mg/100 g dry weight (DW), respectively. The experimental values of total anthocyanins and total phenolic compounds were 88.25 and 1,554.37 mg/100 g DW, respectively, which is well matched with the predicted values within 7.5% difference.

키워드

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