Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Anti-inflammatory and antimicrobial effects of Jeju rosemary essential oil against skin flora

제주산 로즈마리 에센셜 오일의 항염 및 피부 상재균에 대한 항균 활성

  • Kim, So-Hee (Biotechnology Regional Innovation Center, Jeju National University) ;
  • Yi, Mi-Ran (Biotechnology Regional Innovation Center, Jeju National University) ;
  • Kim, Chang-Soo (Biotechnology Regional Innovation Center, Jeju National University) ;
  • Kim, Jung-Mi (UCL Co., Ltd.) ;
  • Bu, Hee-Jung (International Aromatherapy Clinical Jeju Center)
  • 김소희 (제주대학교 생명과학기술혁신센터) ;
  • 이미란 (제주대학교 생명과학기술혁신센터) ;
  • 김창수 (제주대학교 생명과학기술혁신센터) ;
  • 김정미 (유씨엘 주식회사) ;
  • 부희정 (국제아로마테라피임상연구제주센터)
  • Received : 2018.08.03
  • Accepted : 2018.09.20
  • Published : 2018.09.30
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Abstract

The purpose of this study was to investigate the anti-inflammatory effect and the antimicrobial activity to skin flora of essential oil from rosemary that naturally grown in Jeju. rosemary essential oil was extracted by water distillation essential oil extraction method. In order to confirm the anti-inflammatory activity of rosemary essential oil, it was confirmed that the production of NO and $PGE_2$ induced by LPS in RAW 264.7 cells was inhibited in a concentration-dependent manner. Western blot analysis showed that the expression of iNOS and COX-2, which are biosynthetic enzymes, decreased in a concentration-dependent manner. In addition, production of $TNF-{\alpha}$ and IL-6 the pro-inflammatory cytokines were inhibited. Antimicrobial activities of three S. epidermidis and three P. acnes strains including two antibiotic resistant strains were observed in paper disc method and MIC and MBC tests showed inhibition of bacterial growth and death. From the results of the experiment, we confirmed that rosemary essential oil has the anti-inflammatory and antimicrobial efficacy and it could be used as a cosmetic and skin care material in the future.

본 연구는 제주산 자생 로즈마리 에센셜 오일의 항염 및 피부상재균에 대한 항균 활성을 확인하기 위한 것이다. 로즈마리 에센셜 오일은 물 증류법으로 추출하였다. 로즈마리 에센셜 오일의 항염 효능을 확인하기 위하여 RAW 264.7 세포에서 LPS에 의해 유도된 NO와 $PGE_2$의 생성을 농도 의존적으로 억제하는 것을 확인하였다. Western blot 실험을 통해 이들을 생합성하는 효소인 iNOS, COX-2 단백질의 발현이 농도 의존적으로 감소하는 것을 확인하였다. 또한 전염증성 cytokine인 TNF-${\alpha}$와 IL-6의 생성 억제 효능을 확인하였다. 항생제 내성균주 각 2종을 포함한 S. epidermidis 3종, P. acnes 3종에 대한 항균 활성을 실험한 결과, paper disc법에서 저해환이 관찰되었고, MIC, MBC 실험을 통하여 균생육 저해와 사멸를 확인하였다. 위의 실험 결과로부터 로즈마리 에센셜 오일의 항염증, 항균 효능을 확인 하였으며 향후 화장품 및 스킨케어 소재로서 활용 가능성을 확인하였다.

Keywords

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