Journal of Korean Society of Forest Science (한국산림과학회지)

Korean Society of Forest Science (한국산림과학회)
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Optimization Conditions for Cryopreservation of Potentilla discolor Bunge

솜양지꽃(Potentilla discolor Bunge)의 초저온동결보존을 위한 최적 조건 탐색

  • Yang, Woo Hyeong (Division of Environmental Forest Science, Gyeongsang National University (Institute of Agriculture of Life Science)) ;
  • Yong, Seong Hyeon (Division of Environmental Forest Science, Gyeongsang National University (Institute of Agriculture of Life Science)) ;
  • Park, Dongjin (Division of Environmental Forest Science, Gyeongsang National University (Institute of Agriculture of Life Science)) ;
  • Seol, Yuwon (Division of Environmental Forest Science, Gyeongsang National University (Institute of Agriculture of Life Science)) ;
  • Choi, Eunji (Division of Environmental Forest Science, Gyeongsang National University (Institute of Agriculture of Life Science)) ;
  • Jeong, Mi Jin (Plant Conservation Division, Korea National Arboretum of the Korea Forest Service) ;
  • Choi, Myung Suk (Division of Environmental Forest Science, Gyeongsang National University (Institute of Agriculture of Life Science))
  • 양우형 (경상대학교 환경산림과학부) ;
  • 용성현 (경상대학교 환경산림과학부) ;
  • 박동진 (경상대학교 환경산림과학부) ;
  • 설유원 (경상대학교 환경산림과학부) ;
  • 최은지 (경상대학교 환경산림과학부) ;
  • 정미진 (국립수목원 식물보존과) ;
  • 최명석 (경상대학교 환경산림과학부)
  • Received : 2018.04.16
  • Accepted : 2018.09.11
  • Published : 2018.09.30
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Abstract

This study was conducted to investigate the effective cryopreservation condition of Potentilla discolor Bunge, a rare native plant. Seed viability was more than 80% in PVS2 and PVS3 solution treatments. Seed viability was higher in PVS3 than PVS2 treatment. Seed viability was lower than control in spite of sucrose pretreatment. The germination rate was 95% at 60 min of PVS2 treatment and 30 min of PVS3 treatment but the germination rate was low at other treatments. The growth of the seedling wasn't doing that of the control except for treat PVS2 and PVS3 solution for 30 min. There was no statistically significant difference between the encapsulation method and the vitrification method in the seedling growth between the two cryogenic storage methods. This study is expected to be applied to future conservation methods of Potentilla discolor.

본 연구는 희귀자생식물인 솜양지꽃의 효율적인 초저온 보존 조건을 탐색하고자 행하였다. 종자의 활력은 PVS2와 PVS3 용액 처리구에서는 약 80% 이상으로 대조구보다 훨씬 높은 활력을 보였다. 종자의 활력은 PVS3가 PVS2 처리보다 높게 나타났다. 종자의 활력은 sucrose처리에도 불구하고 대조구보다 낮았다. PVS2의 60분 처리구와 PVS3의 30분 처리구에서 95%의 발아율을 보였으나 그 외 처리구에서는 발아율이 낮았다. PVS2와 PVS3 처리구에서 배양된 유묘의 생장은 PVS2와 PVS3 용액 30분 처리구를 제외하고는 대조구에 비해 생장이 좋지 않았다. 두 가지 초저온동결보존법 간 유묘 생장에서는 encapsulation법과 vitrification법을 비교한 결과 미세한 차이를 보였으나 통계적으로는 유의성이 없었다. 본 연구는 솜양지꽃의 보존에 도움이 될 것으로 판단된다.

Keywords

References

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