DOI QR코드

DOI QR Code

Complete genome sequence of Pseudoalteromonas donghaensis HJ51T isolated from seawater

해수에서 분리된 Pseudoalteromonas donghaensis HJ51T 의 유전체 서열분석

  • Oh, Ji-Sung (Department of Microbiology, Chungbuk National University) ;
  • Roh, Dong-Hyun (Department of Microbiology, Chungbuk National University)
  • 오지성 (충북대학교 자연과학대학 미생물학과) ;
  • 노동현 (충북대학교 자연과학대학 미생물학과)
  • Received : 2018.09.07
  • Accepted : 2018.09.17
  • Published : 2018.09.30

Abstract

The whole genome sequencing using PacBio RS II platform was performed for a marine bacterium Pseudoalteromonas donghaensis $HJ51^T$ isolated from East Sea of Korea. As a result, three assembled contigs consisting of a chromosome (size of 3,646,857 bp, and G + C content of 41.8%) and two plasmids (size of 842,855 bp and 244,204 bp, and G + C content of 41.3% and 40.4%, respectively) were obtained. The genome included 4,083 protein coding genes and 127 RNA genes. This result could be used for gene sources of biopolymers degradation and the development as a new host with secretion system similar to Escherichia coli.

이 연구에서는 PacBio RS II 플랫폼을 사용하여 동해에서 분리된 해양 미생물 Pseudoalteromonas donghaensis $HJ51^T$의 전체 유전체 서열화를 수행하였다. 그 결과, 크기 3,646,857 bp, G + C 함량 41.8%인 염색체와 함께 크기 842,855 bp, G + C 함량 41.3% 및 크기 244,204 bp, G + C 함량 40.4%인 두 개의 플라스미드로 구성된 3개의 유전체 서열을 획득하였다. 이 유전체는 4,083개의 단백질 암호화 유전자와 127개의 RNA 유전자를 포함하고 있다. 다양한 생체 고분자 분해효소의 유전자원과 대장균과 유사한 세포외 단백질 분비 숙주로서의 개발에 이용될 수 있을 것으로 생각된다.

Keywords

References

  1. Bowman JP. 2007. Bioactive compound synthetic capacity and ecological significance of marine bacterial genus Pseudoalteromonas. Mar. Drugs 5, 220-241. https://doi.org/10.3390/md504220
  2. de Pascale D, Giuliani M, De Santi C, Bergamasco N, Amoresano A, Carpentieri A, Parrilli E, and Tutino ML. 2010. PhAP protease from Pseudoalteromonas haloplanktis TAC125: gene cloning, recombinant production in E. coli and enzyme characterization. Polar Sci. 4, 285-294. https://doi.org/10.1016/j.polar.2010.03.009
  3. Gauthier G, Gauthier M, and Christen R. 1995. Phylogenetic analysis of the genera Alteromonas, Shewanella, and Moritella using genes coding for small-subunit rRNA sequences and division of the genus Alteromonas into two genera, Alteromonas (emended) and Pseudoalteromonas gen. nov., and proposal of twelve new species combinations. Int. J. Syst. Bacteriol. 45, 755-761. https://doi.org/10.1099/00207713-45-4-755
  4. He H, Chen X, Li J, Zhang Y, and Gao P. 2004. Taste improvement of refrigerated meat treated with cold-adapted protease. Food Chem. 84, 307-311. https://doi.org/10.1016/S0308-8146(03)00242-5
  5. Holmstrom C and Kjelleberg S. 1999. Marine Pseudoalteromonas species are associated with higher organisms and produce biologically active extracellular agents. FEMS Microbiol. Ecol. 30, 285-293. https://doi.org/10.1111/j.1574-6941.1999.tb00656.x
  6. Jeong H, Kim HJ, and Lee SJ. 2015. Complete genome sequence of Escherichia coli strain BL21. Genome Announc. 3, e00134-15.
  7. Kanehisa M, Sato Y, and Morishima K. 2016. BlastKOALA and GhostKOALA: KEGG tools for functional characterization of genome and metagenome sequences. J. Mol. Biol. 428, 726-731. https://doi.org/10.1016/j.jmb.2015.11.006
  8. Lee SO, Kato J, Takiguchi N, Kuroda A, Ikeda T, Mitsutani A, and Ohtake H. 2000. Involvement of an extracellular protease in algicidal activity of the marine bacterium Pseudoalteromonas sp. strain A28. Appl. Environ. Microbiol. 66, 4334-4339. https://doi.org/10.1128/AEM.66.10.4334-4339.2000
  9. Oh JS, Choi YS, and Roh DH. 2015. Characterization and optimum production condition of extracellular protease from Pseudoalteromonas donghaensis HJ51. Korean J. Microbiol. 51, 75-80. https://doi.org/10.7845/kjm.2015.5012
  10. Oh YS, Park AR, Lee JK, Lim CS, Yoo JS, and Roh DH. 2011. Pseudoalteromonas donghaensis sp. nov., isolated from seawater. Int. J. Syst. Evol. Microbiol. 64, 351-355.
  11. Seemann T. 2014. Prokka: rapid prokaryotic genome annotation. Bioinformatics 30, 2068-2069. https://doi.org/10.1093/bioinformatics/btu153
  12. UniProt Consortium. 2017. UniProt: the universal protein knowledgebase. Nucleic Acids Res. 45, D158-D169. https://doi.org/10.1093/nar/gkw1099
  13. Yan BQ, Chen XL, Hou XY, He H, Zhou BC, and Zhang YZ. 2009. Molecular analysis of the gene encoding a cold-adapted halophilic subtilase from deep-sea psychrotolerant bacterium Pseudoalteromonas sp. SM9913: cloning, expression, characterization and function analysis of the C-terminal PPC domains. Extremophiles 13, 725-733. https://doi.org/10.1007/s00792-009-0263-1