미생물학회지 (Korean Journal of Microbiology)

  • 제54권3호
  • /
  • Pages.302-304
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  • 2018
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  • 0440-2413(pISSN)
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  • 2383-9902(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
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히알우론산 유도하에 히알우로네이트 라이아제를 생산하는 Vibrio splendidus KCTC 11899BP균주의 유전체 서열 분석

Draft genome sequences of Vibrio splendidus KCTC 11899BP, which produces hyaluronate lyase in the presence of hyaluronic acid

  • 박주웅 (바이오스트림 테크놀로지스(주)) ;
  • 이상은 (바이오스트림 테크놀로지스(주)) ;
  • 신운섭 (카톨릭관동대학교 의과대학 미생물학과) ;
  • 김경진 (선문대학교 건강보건대학 제약생명공학과) ;
  • 김연욱 (선문대학교 건강보건대학 제약생명공학과)
  • Park, Joo Woong (Biostream Technologies Co.) ;
  • Lee, Sang-Eun (Biostream Technologies Co.) ;
  • Shin, Woon-Seob (Department of Microbiology, Catholic Kwandong University College of Medicine) ;
  • Kim, Kyoung Jin (Department of Pharmaceutical Engineering & Biotechnology, Sun Moon University) ;
  • Kim, Youn Uck (Department of Pharmaceutical Engineering & Biotechnology, Sun Moon University)
  • 투고 : 2018.07.20
  • 심사 : 2018.08.21
  • 발행 : 2018.09.30
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초록

우리는 처음으로 바닷물에서 히알우론산 분해효소를 생산하는 균주인 Vibrio splendidus KCTC 11899BP를 분리하고 동정했다. KCTC 11899BP는 히알우론산(HA)이 기초 배지에 첨가 될 때에만 Hyaluronate lyase를 생산하며, 이 효소는 HA의 ${\beta}$-(1, 4) 결합을 분해하여 이당(disaccharide)을 생성시키는 효소로서 미생물에 의해 생산된다. 게놈 염기서열분석을 통해, KCTC 11899BP의 게놈은 2개의 염색체를 보유하는 다른 Vibrio sp.와 유사하게 각각 3,522 kb (contig 1)와 1,986 kb (contig 2)인 두 개의 원형 contig로 구성되어 있다는 것을 확인하였다. 또한 4,700개의 예측 오픈 리딩 프레임, G + C 함량 44.12%, 137개의 tRNA 유전자 및 46개의 rRNA 유전자를 포함하고 있다는 것을 확인했다.

We, for the first time, isolated and identified a Vibrio splendidus KCTC 11899BP producing hyaluronate lyase from seawater. This enzyme is produced only when hyaluronic acid (HA) is added to the basal medium. Hyaluronate lyases are produced by microorganisms, which degrade the ${\beta}$-(1, 4) bond of HA to produce disaccharide. The genome of KCTC 11899BP, which consist of two circular contigs that are 3,522 kb (contig 1) long and 1,986 kb (contig 2) long respectively, as like other Vibrio sp. that contained 2 chromosomes. The genome included 4,700 predicted open reading frames, G + C content 44.12%, 137 tRNA genes, and 46 rRNA genes.

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참고문헌

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