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Complete genome sequence of multidrug-resistant Moraxella osloensis NP7 with multiple plasmids isolated from human skin

사람의 피부에서 분리한 다약제 내성이며 다수의 플라스미드를 갖는 Moraxella osloensis NP7 균주의 유전체 서열 분석

  • Ganzorig, Munkhtsatsral (Department of Bio Health Science, Changwon National University) ;
  • Lim, Jae Yun (Department of Agricultural Biotechnology, Seoul National University) ;
  • Hwang, Ingyu (Department of Agricultural Biotechnology, Seoul National University) ;
  • Lee, Kyoung (Department of Bio Health Science, Changwon National University)
  • Received : 2018.06.07
  • Accepted : 2018.06.18
  • Published : 2018.09.30

Abstract

Moraxella osloensis NP7 was isolated from human skin of a collage male and showed resistance to ${\beta}-lactam$ and aminoglycoside antibiotics. Herein, we report the complete whole-genome sequence and gene annotations of M. osloensis NP7. It possesses single circular chromosome and seven plasmids. Chromosome is 2,389,582 bp in length with the G + C content of 43.9% and encodes 2,065 protein-coding genes. The combined seven plasmids are 654,202 bp in size with the average G + C content of 40.5% and code for a total of 667 protein-coding genes. The chromosome of NP7 strain contains four ribosomal RNA operon copies, one transfer-messenger RNA gene, forty-seven tRNA genes, three riboswitch genes and three CRISPR arrays. Additional CRISPR array is found in the plasmid pNP7-1. The genes conferring resistance to ${\beta}-lactam$ and aminoglycoside antibiotics were predicted to reside in the plasmid pNP7-1.

남자 대학생의 피부에서 분리한 Moraxella osloensis NP7는 베타-락탐과 아미노글리코사이드 항생제에 대해 내성을 보였다. 본 연구에서는 NP7 균주 유전체의 완전한 염기서열과 유전자 주석을 보고하고자 한다. NP7 균주는 원형 염색체와 7개의 플라스미드를 갖고 있다. 염색체는 43.9%의 G + C 함량을 갖는 2,389,582개의 염기쌍을 갖고 있으며, 단백질을 암호하는 2,065개의 유전자를 보유하고 있다. 전체 플라스미드는 평균적으로 40.5%의 G + C 함량을 갖는 654,202개의 염기쌍을 갖고 있으며, 단백질을 암호하는 667개의 유전자를 보유하고 있다. 염색체는 4개의 리보좀 RNA 오페론, 1개의 transfermessenger RNA 유전자, 47개의 tRNA 유전자, 3개의 핵산스위치 유전자 그리고 3개의CRISPR array를 포함하고 있으며, 1개의 CRISPR은 pNP7-1 플라스미드에 존재한다. 베타-락탐과 아미노글리코사이드 항생제에 내성을 부여하는 유전자는 pNP7-1 플라스미드에 존재하고 있다.

Keywords

References

  1. Ausubel F, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, and Struhl K. 1990. Current protocols in molecular biology. John Wiely and Sons, New York, NY, USA.
  2. Besemer J, Lomsadze A, and Borodovsky M. 2001. GeneMarkS: a self-training method for prediction of gene starts in microbial genomes. Implications for finding sequence motifs in regulatory regions. Nucleic Acids Res. 29, 2607-2618. https://doi.org/10.1093/nar/29.12.2607
  3. Cardinale M, Kaiser D, Lueders T, Schnell S, and Egert M. 2017. Microbiome analysis and confocal microscopy of used kitchen sponges reveal massive colonization by Acinetobacter, Moraxella and Chryseobacterium species. Sci. Rep. 7, 5791. https://doi.org/10.1038/s41598-017-06055-9
  4. Cosseau C, Romano-Bertrand S, Duplan H, Lucas O, Ingrassia I, Pigasse C, Roques C, and Jumas-Bilak E. 2016. Proteobacteria from the human skin microbiota: species-level diversity and hypotheses. One Health 2, 33-41. https://doi.org/10.1016/j.onehlt.2016.02.002
  5. Dien Bard J, Lewinski M, Summanen PH, and Deville JG. 2011. Sepsis with prolonged hypotension due to Moraxella osloensis in a non-immunocompromised child. J. Med. Microbiol. 60, 138-141. https://doi.org/10.1099/jmm.0.016378-0
  6. Han XY and Tarrand JJ. 2004. Moraxella osloensis blood and catheter infections during anticancer chemotherapy: clinical and microbiologic studies of 10 cases. Am. J. Clin. Pathol. 121, 581-587. https://doi.org/10.1309/QBB3AVCMGWA3K1XK
  7. Kubota H, Mitani A, Niwano Y, Takeuchi K, Tanaka A, Yamaguchi N, Kawamura Y, and Hitomi J. 2012. Moraxella species are primarily responsible for generating malodor in laundry. Appl. Environ. Microbiol. 78, 3317-3324. https://doi.org/10.1128/AEM.07816-11
  8. Lim JY, Hwang I, Ganzorig M, Huang SL, Cho GS, Franz C, and Lee K. 2018. Complete genome sequences of three Moraxella osloensis strains isolated from human skin. Genome Announc. 6, e01509-17.
  9. Lim JY, Lee K, and Hwang I. 2014. Complete genome sequence of the mushroom-like aerial structure-forming Pseudomonas alkylphenolia, a platform bacterium for mass production of poly-${\beta}$-D-mannuronates. J. Biotechnol. 192, 20-21. https://doi.org/10.1016/j.jbiotec.2014.09.024
  10. Stanier RY, Palleroni NJ, and Doudoroff M. 1966. The aerobic pseudomonads: a taxomonic study. J. Gen. Microbiol. 43, 159-271. https://doi.org/10.1099/00221287-43-2-159