Food Engineering Progress (산업식품공학)

Korean Society for Industrial Food Engineering (한국산업식품공학회)
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PCR Analysis for Halal Authentication of Cosmetics Containing Carrot, Oyster Mushroom, and Pine Needle Extracts

당근, 느타리버섯 및 솔잎 추출물이 첨가된 화장품의 할랄인증을 위한 PCR 분석

  • Jang, Chan Song (Department of Food Science and Biotechnology, College of Life Science and Biotechnology, Dongguk University) ;
  • Kim, Tae Hyeon (Department of Food Science and Biotechnology, College of Life Science and Biotechnology, Dongguk University) ;
  • Kim, Yu Song (Department of Food Science and Biotechnology, College of Life Science and Biotechnology, Dongguk University) ;
  • Lee, Beom Zoo (Chemland Co. Ltd.) ;
  • Hong, Kwang Won (Department of Food Science and Biotechnology, College of Life Science and Biotechnology, Dongguk University)
  • 장찬송 (동국대학교 식품생명공학과) ;
  • 김태현 (동국대학교 식품생명공학과) ;
  • 김유송 (동국대학교 식품생명공학과) ;
  • 이범주 ((주) 케미랜드) ;
  • 홍광원 (동국대학교 식품생명공학과)
  • Received : 2018.09.06
  • Accepted : 2018.10.24
  • Published : 2018.11.30
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Abstract

In recent years, interest in halal authentication from the domestic food and cosmetics field has been growing for advances into the overseas halal market. For halal authentication, the product must not contain haram ingredients derived from pig, dog, human, GMO, etc. In this study, the presence of haram ingredients in plant extracts (carrot, oyster mushroom, and pine needle) treated with papain and bromelain and cosmetics (mask pack and cream) containing these extracts were analyzed by PCR to confirm whether these cosmetics were suitable for halal authentication. Detection limits of the PCR method that specifically detected template DNA of human, pig, dog, and GMO were $1.29{\times}10^3$, $1.14{\times}10^3$, $1.24{\times}10^2$ and $2.02{\times}10^3copies/tube$, respectively. PCR was not inhibited by the plant extracts or cosmetic ingredients. Results of PCR for the plant extracts or cosmetics containing these extracts were all negative. This PCR method could be used to rapidly identify the presence of haram ingredients in raw materials or final products during the manufacturing process of food and cosmetics.

최근 국내의 식품 및 화장품 분야에서 해외 할랄시장 진출을 위해 할랄 인증에 대한 관심이 높아지고 있다. 할랄 인증을 받으려면 해당 제품에 돼지, 개, 사람, GMO 등과 같은 haram 성분들이 포함되어서는 안 된다. 본 연구에서는 papain과 bromelain을 처리한 식물추출물(당근, 느타리 버섯, 솔잎)이 첨가된 화장품(cream과 mask pack)이 할랄 인증에 적합한지를 확인하기 위해 이들 추출물과 최종제품에서 haram 성분의 존재 여부를 PCR을 이용하여 분석하였다. 사람, 돼지, 개 및 GMO 대두의 template DNA를 특이적으로 검출하는 PCR 방법의 검출한계는 각각 $1.29{\times}10^3$, $1.14{\times}10^3$, $1.24{\times}10^2$ 및 $2.02{\times}10^3copies/tube$ 이었다. PCR은 식물 추출물이나 화장품 성분에 의해 저해 받지 않았다. 식물추출물과 이들이 첨가된 cream과 mask pack에 대해 PCR을 수행한 결과 모두 음성이었다. 본 PCR 방법은 식품이나 화장품의 제조과정 중 원재료나 최종제품에서 haram 성분의 존재를 신속하게 확인하는 데 활용이 가능할 것으로 보인다.

Keywords

Acknowledgement

Supported by : 농림식품기술기획평가원

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