Biomaterials Research (생체재료학회지)

Korean Society for Biomaterials (한국생체재료학회)
권호 표지
DOI QR코드

DOI QR Code

Biogenic fabrication and characterization of silver nanoparticles using aqueous-ethanolic extract of lichen (Usnea longissima) and their antimicrobial activity

  • Siddiqi, Khwaja Salahuddin (Department of Chemistry, Aligarh Muslim University) ;
  • Rashid, M. (Department of Saidla, Aligarh Muslim University) ;
  • Rahman, A. (Department of Saidla, Aligarh Muslim University) ;
  • Tajuddin, Tajuddin (Department of Saidla, Aligarh Muslim University) ;
  • Husen, Azamal (Department of Biology, College of Natural and Computational Sciences, University of Gondar) ;
  • Rehman, Sumbul (Department of Ilmul Advia (Unani Pharmacy), Aligarh Muslim University)
  • Received : 2018.05.17
  • Accepted : 2018.09.03
  • Published : 2018.12.31
⟨ Previous Next ⟩

Abstract

Background: Biogenic fabrication of silver nanoparticles from naturally occurring biomaterials provides an alternative, eco-friendly and cost-effective means of obtaining nanoparticles. It is a favourite pursuit of all scientists and has gained popularity because it prevents the environment from pollution. Our main objective to take up this project is to fabricate silver nanoparticles from lichen, Usnea longissima and explore their properties. In the present study, we report a benign method of biosynthesis of silver nanoparticles from aqueous-ethanolic extract of Usnea longissima and their characterization by ultraviolet-visible (UV-vis), Fourier transform infrared (FTIR) spectroscopy, transmission electron microscopy (TEM) and scanning electron microscopy (SEM) analyses. Silver nanoparticles thus obtained were tested for antimicrobial activity against gram positive bacteria and gram negative bacteria. Results: Formation of silver nanoparticles was confirmed by the appearance of an absorption band at 400 nm in the UV-vis spectrum of the colloidal solution containing both the nanoparticles and U. longissima extract. Poly(ethylene glycol) coated silver nanoparticles showed additional absorption peaks at 424 and 450 nm. FTIR spectrum showed the involvement of amines, usnic acids, phenols, aldehydes and ketones in the reduction of silver ions to silver nanoparticles. Morphological studies showed three types of nanoparticles with an abundance of spherical shaped silver nanoparticles of 9.40-11.23 nm. Their average hydrodynamic diameter is 437.1 nm. Results of in vitro antibacterial activity of silver nanoparticles against Staphylococcus aureus, Streptococcus mutans, Streptococcus pyrogenes, Streptococcus viridans, Corynebacterium xerosis, Corynebacterium diphtheriae (gram positive bacteria) and Escherichia coli, Klebsiella pneuomoniae and Pseudomonas aeruginosa (gram negative bacteria) showed that it was effective against tested bacterial strains. However, S. mutans, C. diphtheriae and P. aeruginosa were resistant to silver nanoparticles. Conclusion: Lichens are rarely exploited for the fabrication of silver nanoparticles. In the present work the lichen acts as reducing as well as capping agent. They can therefore, be used to synthesize metal nanoparticles and their size may be controlled by monitoring the concentration of extract and metal ions. Since they are antibacterial they may be used for the treatment of bacterial infections in man and animal. They can also be used in purification of water, in soaps and medicine. Their sustained release may be achieved by coating them with a suitable polymer. Silver nanoparticles fabricated from edible U. longissima are free from toxic chemicals and therefore they can be safely used in medicine and medical devices. These silver nanoparticles were stable for weeks therefore they can be stored for longer duration of time without decomposition.

Keywords

References

  1. Siddiqi KS, Husen A, Sohrab SS, Osman M. Recent status of nanomaterials fabrication and their potential applications in neurological disease management. Nano Res Lett. 2018;13:231. https://doi.org/10.1186/s11671-018-2638-7
  2. Husen A, Siddiqi KS. Phytosynthesis of nanoparticles: concept, controversy and application. Nano Res Lett. 2014;9:229. https://doi.org/10.1186/1556-276X-9-229
  3. Husen A, Siddiqi KS. Plants and microbes assisted selenium nanoparticles: characterization and application. J Nanobiotechnol. 2014;12:28. https://doi.org/10.1186/s12951-014-0028-6
  4. Husen A, Siddiqi KS. Carbon and fullerene nanomaterials in plant system. J Nanobiotechnol. 2014;12:16. https://doi.org/10.1186/1477-3155-12-16
  5. Siddiqi KS, Husen A. Fabrication of metal nanoparticles from fungi and metal salts: scope and application. Nano Res Lett. 2016;11:98. https://doi.org/10.1186/s11671-016-1311-2
  6. Siddiqi KS, Husen A. Fabrication of metal and metal oxide nanoparticles by algae and their toxic effects. Nano Res Lett. 2016;11:363. https://doi.org/10.1186/s11671-016-1580-9
  7. Siddiqi, Husen A. Engineered gold nanoparticles and plant adaptation potential. Nano Res Lett. 2016;11:400. https://doi.org/10.1186/s11671-016-1607-2
  8. Siddiqi KS, Husen A. Green synthesis, characterization and uses of palladium/platinum nanoparticles. Nano Res Lett. 2016;11:482. https://doi.org/10.1186/s11671-016-1695-z
  9. Siddiqi KS, Rahman A, Tajuddin HA. Biogenic fabrication of iron/iron oxide nanoparticles and their application. Nano Res Lett. 2016;11:498. https://doi.org/10.1186/s11671-016-1714-0
  10. Siddiqi KS, Husen A. Recent advances in plant-mediated engineered gold nanoparticles and their application in biological system. J Trace Elements Med Biol. 2017;40:10-23. https://doi.org/10.1016/j.jtemb.2016.11.012
  11. Siddiqi KS, Husen A, Rao RAK. A review on biosynthesis of silver nanoparticles and their biocidal properties. J Nanobiotechnol. 2018;16:14. https://doi.org/10.1186/s12951-018-0334-5
  12. Siddiqi KS, Rahman A, Tajuddin HA. Properties of zinc oxide nanoparticles and their activity against microbes. Nano Res Lett. 2018;13:141. https://doi.org/10.1186/s11671-018-2532-3
  13. Tagad CK, Dugasani SR, Aiyer R, Park S, Kulkarni A, Sabharwal S. Green synthesis of silver nanoparticles and their application for the development of optical fiber based hydrogen peroxide sensor. Sensors Actuators B Chem. 2013;183:144-9. https://doi.org/10.1016/j.snb.2013.03.106
  14. Venkateswarlu S, Kumar BN, Prathima B, Anitha K, Jyothi NVV. A novel green synthesis of Fe3O4-ag core shell recyclable nanoparticles using Vitis vinifera stem extract and its enhanced antibacterial performance. Physica B. 2015;457:30-5. https://doi.org/10.1016/j.physb.2014.09.007
  15. Rao Y, Kotakadi VS, Prasad TNVKV, Reddy AV, Sai Gopal DVR. Green synthesis and spectral characterization of silver nanoparticles from Lakshmi tulasi (Ocimum sanctum) leaf extract. Spectrochim Acta A. 2013;103:156-9. https://doi.org/10.1016/j.saa.2012.11.028
  16. Husen A. Gold nanoparticles from plant system: synthesis, characterization and their application. In: Ghorbanpourn M, Manika K, Varma A, editors. Nanoscience and plant-soil systems, vol. 48. Switzerland: Springer international publishing AG, Gewerbestrasse 11, 6330 Cham; 2017. p. 455-79.
  17. Dhand V, Soumya L, Bharadwaj S, Chakra S, Bhatt D, Sreedhar B. Green synthesis of silver nanoparticles using Coffea arabica seed extract and its antibacterial activity. Mat Sci Eng C. 2016;58:36-43. https://doi.org/10.1016/j.msec.2015.08.018
  18. Latha M, Priyanka M, Rajasekar P, Manikandan R, Prabhu NM. Biocompatibility and antibacterial activity of the Adathoda vasica Linn extract mediated silver nanoparticles. Microb Pathog. 2016;93:88-94. https://doi.org/10.1016/j.micpath.2016.01.013
  19. Tran TA, Kinch L, PenaLlopis S, Kockel L, Grishin N, Jiang H, Brugarolas J. Platelet-derived growth factor/vascular endothelial growth factor receptor inactivation by sunitinib results in Tsc1/Tsc2-dependent inhibition of TORC1. Mol Cell Biol. 2013;33:3762-79. https://doi.org/10.1128/MCB.01570-12
  20. Austin B, Austin DA. Bacterial fish pathogens. Diseases of farmed and wild fish, springer-praxis publishing, ltd., United Kingdom, 1999.
  21. Cai JP, Li J, Thompson KD, Li CX, Han HC. Isolation and characterization of pathogenic Vibrio parahaemolyticus from diseased post-larvae of abalone Haliotis diversicolor supertexta. J Basic Microbiol. 2007;47:84-6. https://doi.org/10.1002/jobm.200610192
  22. Jayasree L, Janakiram P, Madhavi R. Characterization of Vibrio spp. associated with diseased shrimp from culture ponds of Andhra Pradesh (India). J World Aquacult Soc. 2006;37:523-32. https://doi.org/10.1111/j.1749-7345.2006.00066.x
  23. Yu X, Guo Q, Su G, Yang A, Hu Z, Qu C, Wan Z, Li R, Tu P, Chai X. Usnic acid derivatives with cytotoxic and antifungal activities from the lichen Usnea longissima. J Nat Prod. 2016;79:1373-80. https://doi.org/10.1021/acs.jnatprod.6b00109
  24. Favreau JT, Ryu ML, Braunstein G, Orshansky G, Park SS, Goody GL, Love L, Fong TL. Severe hepatotoxicity associated with the dietary supplement LipoKinetix. Ann Intern Med. 2002;136:590-5. https://doi.org/10.7326/0003-4819-136-8-200204160-00008
  25. Neff GW, Reddy KR, Durazo FA, Meyer D, Marrero R, Kaplowitz N. Severe hepatotoxicity associated with the use of weight loss diet supplements containing ma huang or usnic acid. J Hepatol. 2004;41:1062-4. https://doi.org/10.1016/j.jhep.2004.06.028
  26. Guo L, Shi Q, Fang JL, Mei N, Ali AA, Lewis SM, Leakey JEA, Frankos VH. Review of usnic acid and Usnea barbata toxicity. J Environ Sci Health C Environ Carcinog Ecotoxicol Rev. 2008;26:317-38. https://doi.org/10.1080/10590500802533392
  27. Wei JC, Wang XY, Wu JL, Wu JN, Chen XL, Hou JL. Lichenes Officinales Sinenses. Beijing: Science press; 1982. p. 18-58.
  28. Nishitoba Y, Nishimura I, Nishiyama T, Mizutani J. Lichen acids, plant growth inhibitors from Usnea longissima. Phytochemistry. 1987;26:3181-5. https://doi.org/10.1016/S0031-9422(00)82466-8
  29. Halici M, Odabasoglu F, Suleyman H, Cakir A, Aslan A, Bayir Y. Effects of water extract of Usnea longissima on antioxidant enzyme activity and mucosal damage caused by indomethacin in rats. Phytomedicine. 2005;12:656-62. https://doi.org/10.1016/j.phymed.2004.06.021
  30. Fernandez-Moriano C, Gomez-Serranillos MP, Crespo A. Antioxidant potential of lichen species and their secondary metabolites. A systematic review. Pharm Biol. 2016;54:1-17. https://doi.org/10.3109/13880209.2014.1003354
  31. Luzina OA, Salakhutdinov NF. Biological activity of usnic acid and its derivatives: part 1. Activity against unicellular organisms. Rus J Bioorg Chem. 2016;42:115-32. https://doi.org/10.1134/S1068162016020084
  32. Luzina OA, Salakhutdinov NF. Biological activity of usnic acid and its derivatives: part 2. Effects on higher organisms. Molecular and physicochemical aspects. Rus J Bioorg Chem. 2016;42:249-68. https://doi.org/10.1134/S1068162016030109
  33. Yamamoto Y, Miura Y, Kinoshita Y, Higuchi M, Yamada Y, Murakami A, Ohigashi H, Koshimizu K. Screening of tissue cultures and thalli of lichens and some of their active constituents for inhibition of tumor promoter-induced Epstein-Barr virus activation. Chem Pharm Bull (Tokyo). 1995;43:1388-90. https://doi.org/10.1248/cpb.43.1388
  34. Odabasoglu F, Aslan A, Cakir A, Suleyman H, Karagoz Y, Halici M, Bayir Y. Comparison of antioxidant activity and phenolic content of three lichen species. Phytother Res. 2004;18:938-41. https://doi.org/10.1002/ptr.1488
  35. Turhan K, Ekinci-Dogan C, Akcin G, Aslan A. Biosorption of au(III) and cu(II) from aqueous solution by a non-living Usnea longissima biomass. Fres Environ Bull. 2005;14:1129-35.
  36. Eugino M, Muller N, Frases S, Almeida-Paes R, Mauricio LMTR, Lemgruber L, Farina M, de-Souza W, Anna CS. Test-derived biosynthesis of silver/silver chloride nanoparticles and their antiproliferative activity against bacteria. RSC Adv. 2016;6:9893-904. https://doi.org/10.1039/C5RA22727E
  37. Rajput S, Werezuk R, Lange RM, McDermott MT. Fungal isolate optimized for biogenesis of silver nanoparticles with enhanced colloidal stability. Langmuir. 2016;32:8688-97. https://doi.org/10.1021/acs.langmuir.6b01813
  38. Zhang JZ, Nogues C. Plasmonic optical properties and applications of metal nanostructures. Plasmonics. 2008;3:127-50. https://doi.org/10.1007/s11468-008-9066-y
  39. Rajakumar G, Gomathi T, Thiruvengadam M, Rajeswari VD, Kalpana VN, Chung IM. Evaluation of anti-cholinesterase, antibacterial and cytotoxic activities of green synthesized silver nanoparticles using from Millettia pinnata flower extract. Microb Pathogen. 2017;103:123-8. https://doi.org/10.1016/j.micpath.2016.12.019
  40. Kalimuthu K, Suresh Babu R, Venkataraman D, Bilal M, Gurunathan S. Biosynthesis of silver nanocrystals by Bacillus licheniformis. Colloids Surf B Biointerf. 2017;65:150-3.
  41. Vijay Kumar PPN, Pammi SVN, Kollu P, Satyanarayana KVV, Shameem U. Green synthesis and characterization of silver nanoparticles using Boerhaavia diffusa plant extract and their anti bacterial activity. Ind Crop Prod. 2004;52:562-6.
  42. Nakamoto K. Infrared and Raman spectra of inorganic and coordination compounds, part a and part B, 2 Vol set, 6thEdition. John Wiley & Sons, Inc. USA, 2009.
  43. Prabhu S, Poulose EK. Silver nanoparticles: mechanism of antimicrobial action, synthesis, medical applications, and toxicity effects. Int Nano Lett. 2012;2:32. https://doi.org/10.1186/2228-5326-2-32
  44. Hong X, Wen J, Xiong X, Hu Y. Shape effect on the antibacterial activity of silver nanoparticles synthesized via a microwave-assisted method. Environ Sci Pollut Res. 2016;23:4489-97. https://doi.org/10.1007/s11356-015-5668-z
  45. Agnihotri S, Mukherji S, Mukherji S. Size-controlled silver nanoparticles synthesized over the range 5-100 nm using the same protocol and their antibacterial efficacy. RSC Adv. 2014;4:3974-83. https://doi.org/10.1039/C3RA44507K
  46. Saravanan M, Amelash T, Negash L, Gebreyesus A, Selvaraj A, Rayar V. Deekonda K extracellular biosynthesis and biomedical application of silver nanoparticles synthesized from Baker's yeast. Int J Res Pharm Biomed Sci. 2013;4:822-8.
  47. Bonnigala B, Aswani Kumar YVV, Vinay Viswanath K, Joy Richardson P, Mangamuri UK, Poda S. Anticancer activity of plant mediated silver nanoparticles on selected cancer cell lines. J Chem Pharma Res. 2016;8:276-81.

Cited by

  1. Biofabrication of Silver Nanoparticles from Diospyros montana, Their Characterization and Activity Against Some Clinical Isolates vol.9, pp.2, 2018, https://doi.org/10.1007/s12668-019-00629-9
  2. Antibacterial, Antibiofilm, Antiquorum Sensing, Antimotility, and Antioxidant Activities of Green Fabricated Ag, Cu, TiO2, ZnO, and Fe3O4 NPs via Protoparmeliopsis mur vol.5, pp.9, 2018, https://doi.org/10.1021/acsbiomaterials.9b00274
  3. Biosynthesis, Characterization, and Biological Activities of Procyanidin Capped Silver Nanoparticles vol.11, pp.3, 2018, https://doi.org/10.3390/jfb11030066
  4. Biomechanochemical Solid-State Synthesis of Silver Nanoparticles with Antibacterial Activity Using Lichens vol.8, pp.37, 2018, https://doi.org/10.1021/acssuschemeng.0c03211
  5. Bio fabrication of silver nanoparticles with antibacterial and cytotoxic abilities using lichens vol.10, pp.1, 2018, https://doi.org/10.1038/s41598-020-73683-z
  6. Review on green nano-biosynthesis of silver nanoparticles and their biological activities: with an emphasis on medicinal plants vol.51, pp.1, 2021, https://doi.org/10.1080/24701556.2020.1769662
  7. A Mini-Review on Lichen-Based Nanoparticles and Their Applications as Antimicrobial Agents vol.12, pp.None, 2021, https://doi.org/10.3389/fmicb.2021.633090
  8. Lichens-A Potential Source for Nanoparticles Fabrication: A Review on Nanoparticles Biosynthesis and Their Prospective Applications vol.7, pp.4, 2018, https://doi.org/10.3390/jof7040291
  9. A comprehensive review on secondary metabolites and health-promoting effects of edible lichen vol.80, pp.None, 2018, https://doi.org/10.1016/j.jff.2020.104283
  10. Biological Potential of Silver Nanoparticles Mediated by Leucophyllum frutescens and Russelia equisetiformis Extracts vol.11, pp.8, 2018, https://doi.org/10.3390/nano11082098
  11. Silver Nanoparticles Formation by Jatropha integerrima and LC/MS-QTOF-Based Metabolite Profiling vol.11, pp.9, 2018, https://doi.org/10.3390/nano11092400
  12. Optimization of silver nanoparticles synthesis by the green method using Streptomyces sp. SSUT88A and their antimicrobial activity against Pseudomonas aeruginosa vol.948, pp.1, 2018, https://doi.org/10.1088/1755-1315/948/1/012085