Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Development of a Multiplex RT-PCR for the Simultaneous Detection of Three Viruses in Cherry Plants

  • Park, Chung Youl (Seed Testing & Research Center, Korea Seed & Variety Service) ;
  • Park, Jeongran (Seed Testing & Research Center, Korea Seed & Variety Service) ;
  • Lee, Geunsik (Seed Testing & Research Center, Korea Seed & Variety Service) ;
  • Yi, Seung-In (Seed Testing & Research Center, Korea Seed & Variety Service) ;
  • Kim, Byeong Hoon (Daegu Agricultural Technology Center) ;
  • Eom, Jung Sik (Daegu Agricultural Technology Center) ;
  • Lee, Som Gyeol (Daegu Agricultural Technology Center) ;
  • Kim, Hongsup (Seed Testing & Research Center, Korea Seed & Variety Service)
  • Received : 2018.06.28
  • Accepted : 2018.07.18
  • Published : 2018.09.30
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Abstract

A multiplex RT-PCR (mRT-PCR) assay was developed for the detection of the recently reported viruses, Cherry virus A (CVA), Little cherry virus 1 (LChV-1), and Little cherry virus 2 (LChV-2), in cherry plants in Korea. Eight sets of primers were designed for each virus and their specificity was tested by using various combinations of mixed primer sets. From the designed primer sets, one combination was selected and further evaluated to estimate the optimum temperature and detection limits of the mRT-PCR. A newly developed mRT-PCR assay was also tested using 20 cherry samples collected in the field. This mRT-PCR assay may be a useful tool for field surveys of diseases and the rapid detection of these three viruses in cherry plants.

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References

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