Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Antimicrobial Activity Screening of Sixty-four Evergreen Woody Species According to Extraction Conditions against Trichophyton mentagrophytes

상록성 목본 64종의 추출조건에 따른 무좀원인균의 항균활성 스크리닝

  • Jang, Bo Kook (Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural, and Food Sciences, Chungbuk National University) ;
  • Chi, Lai Won (Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural, and Food Sciences, Chungbuk National University) ;
  • Cho, Ju Sung (Quality Control & Seed Tech Team, Nongwoo Bio CO., LTD.) ;
  • Lee, Cheol Hee (Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural, and Food Sciences, Chungbuk National University)
  • 장보국 (충북대학교 축산.원예.식품공학부 생물건강소재산업화사업단) ;
  • 지래원 (충북대학교 축산.원예.식품공학부 생물건강소재산업화사업단) ;
  • 조주성 ((주)농우바이오 품질관리기술팀) ;
  • 이철희 (충북대학교 축산.원예.식품공학부 생물건강소재산업화사업단)
  • Received : 2018.03.19
  • Accepted : 2018.05.28
  • Published : 2018.08.31
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Abstract

This study was performed to investigate and measure the antimicrobial activity of evergreen woody species extracts on Trichophyton mentagrophytes. To do this, leaves and stems were collected from Wando and Jeju islands, and were used for the extraction with different solvents (i.e., distilled water, 80% ethanol, and 100% methanol), and at different ultrasonic extracting times (i.e., 15, 30, and 45 minutes). The experiment was conducted by using the agar diffusion method. The clear zone was measured after incubating the paper disc containing the plant extract in a bacterial culture medium. The controls were synthetic antimicrobials, methylparaben and phenoxyethanol, at concentrations of 0.4, 1, 2, and 4 mg/disc. Altogether, extracts of 56 out of 64 species used in this study had inhibitory activity, which confirmed their antimicrobial activity against Athlete's foot. Among them, the crude ethanolic extract of Elaeocarpus sylvestris in 45 min showed a zone of inhibition < 20.2 mm, while the clear zone of Actinodaphne lancifolia ethanolic extraction for 30 min was 23.5 mm. Also, Quercus acuta, Dendropanax morbiferus and Daphne odora showed clear zones of 28.0 mm (45 minutes ethanolic extraction), 20.5 mm (45 minutes crude methanolic extraction) and 19.7 mm (45 minutes methanolic extraction), respectively. Thus, these results confirm that the extracts of evergreen woody species have therapeutic potential against Athlete's foot, and suggest that in order to extract adequate amounts of antimicrobial substance from the plant sources, ideal extraction condition has to be considered.

본 연구는 무좀균(Trichophyton mentagrophytes)에 대한 상록성 목본 추출물의 항균활성을 조사하고 측정하기 위해 수행되었다. 이를 위해 완도와 제주도에서 채집한 잎과 줄기를 용매(증류수, 80% 에탄올, 100% 메탄올)와 초음파 처리시간(15, 30, 45분)을 달리하여 추출하여 실험에 사용하였다. 실험은 한천확산법을 사용하여, 박테리아 배지에 식물 추출물이 함유된 종이디스크를 배양한 뒤클리어존(생육억제환)을 측정하였다. 대조군은 합성항균제인 methylparaben과 phenoxyethanol 0.4, 1, 2, 4 mg/disc에 농도로 사용하였다. 연구에 사용된 64종 중 56종의 추출물에서 클리어존이 보여, 무좀균에 대한 항균작용을 확인할 수 있었다. 그 중 담팔수는 에탄올에 45분간 추출한 처리구에서 20.2 mm, 육박나무는 80% 에탄올로 30분간 추출한 처리구에서 23.5 mm의 클리어존을 나타냈다. 또한 붉가시나무, 황칠나무 및 서향의 잎 추출물은 각각 28.0 mm (80% 에탄올 45분 추출), 20.5 mm (100% 메탄올 45분 추출) 및 19.7 mm (100% 메탄올 45분 추출)의 클리어존이 조사되었다. 따라서, 이러한 결과는 상록성 목본 추출물의 무좀균에 대한 치료 가능성을 확인할 수 있었으며, 항균물질이 많이 함유한 식물소재를 얻기 위해서는 식물의 적정 추출조건을 고려해야 할 것으로 생각된다.

Keywords

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