Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
권호 표지
DOI QR코드

DOI QR Code

Modulative Effect of Human Hair Dermal Papilla Cell Apoptosis by Oregonin from the Braches of Alnus japonica

오리나무 가지 유래 Oregonin의 인체 모유두 세포 Apoptosis 조절 효능

  • Lee, Gyeong Hwa (Department of Cosmetology Science, Nambu University) ;
  • Park, Kwang Hyun (Department of Oriental Pharmaceutical Development, Nambu University) ;
  • Choi, Sun Eun (Department of Cosmetology Science, Nambu University)
  • 이경화 (남부대학교 향장미용학과) ;
  • 박광현 (남부대학교 한방제약개발학과) ;
  • 최선은 (남부대학교 향장미용학과)
  • Received : 2018.02.08
  • Accepted : 2018.05.18
  • Published : 2018.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

A diarylheptanoid, (5S)-1,7-bis-(3,4-dihydroxyphenyl)-5-hydroxyheptane-3-one-5-O-${\beta}$-D-xylopyranoside, named oregonin (1), was isolated from the of Alnus japonica (A. japonica), which is a species of the genus Betulaceae, growing throughout Korea, Japan and China. The structure was elucidated by various spectroscopic methods including negative and positive LC/MS, 1H-NMR, and 13C-NMR techniques or by comparison with authentic samples. In order to evaluate the anti-oxidative activities of oregonin (1) isolated from A. japonica, 1,1-diphenyl-2-picryhydrazyl (DPPH) radical scavenging activity and 2,2'-azino-bis[3-ethylbenzothiazoline-6-sulphonic acid] (ABTS) radical scavenging activity were measured in vitro. Oregonin from A. japonica exhibited potent DPPH and ABTS radical scavenging activities. A. japonica shows not only 1,1-diphenyl-2-picryhydrazyl (DPPH) radical scavenging activity and ABTS radical scavenging activity, but also apoptosis modulative effects. The present results indicate that A. japonica could be a hair-growth-promoting agent for cosmetic products.

최근 식품의약품안전처에서 그동안 의약외품으로 분류되었던 '탈모 증상 완화' 관련 제품이 기능성 화장품의 범주에 포함됨에 따라서 관련 산업계에 큰 관심 중 하나라고 할 수 있다. 본 연구에서는 기존 의약품들의 한계점인 부작용의 위험률이 적으면서 탈모를 과학적으로 조절시켜줄 수 있는 객관적인 근거를 제시할 수 있는 천연물 유래 신소재를 발굴하고자, 오리나무 가지로부터 분리한 oregonin은 강력한 활성산소종 소거능을 가지며 탈모와 연관성이 높은 모유두 세포의 사멸을 명확하게 조절함을 확인하였다. 결론적으로 oregonin을 포함한 오리나무 추출물은 두피에서 발생한 활성산소를 강력하게 제거하는 효능을 기반으로 하여, 산화적 스트레스에 의한 모유두세포의 사멸 억제 및 조절을 통하여 탈모방지 효과를 기대할 수 있는 천연 탈모 방지 및 양모 신소재로서의 개발가능성이 있는 것으로 확인하였다.

Keywords

References

  1. Alnemri, E.S., D.J. Livingston, D.W. Nicholson, G. Salvesen, N.A. Thornberry, W.W. Wong and J. Yuan. 1996. Human ICE/CED-3 protease nomenclature. Cell 87:171.
  2. Alnermri, E.S. 1997. Mammalian cell death proteases: a family of highly conserved aspartate specific cystein proteases. J. Cell. Biochem. 64:32-42.
  3. Boericke, W. 1927. Pocket Manual of Homeopathic Materia Medica with Repertory (Ninth Edition). Boericke & Tafel, INC. Santa Rosa, CA (USA). p. 24.
  4. Boivin, W.A., H. Jiang, O.B. Utting and D.W.C. Hunt. 2006. Influence of interleukin-$1{\alpha}$ on androgen receptor expression and cytokine secretion by cultured human dermal papilla cells. Exp. Dermatol. 15:784-793.
  5. Burnette, W.N. 1981. Western blotting: electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal. Biochem. 112:195-203.
  6. Choi, S.E. 2013. Chemotaxonomic significance of oregonin in Alnus species. Asian J. Chem. 25:6989.
  7. Choi, S.E., K.H. Park, M.H. Kim, J.H. Song, H.Y. Jin and M.W. Lee. 2012. Diarylheptanoids from the Bark of Alnus pendula Matsumura. Nat. Prod. Sci. 18:106.
  8. Choi, S.E., M.S. Jeong, M.J. Kang, D.I. Lee, S.S. Joo, C.S. Lee, H. Bang, M.K. Lee, S.C. Myung, Y.W. Choi, K. Lee, S.J. Seo and M.W. Lee. 2010. Effect of topical application and intraperitoneal injection of oregonin on atopic dermatitis in NC/Nga mice. Exp. Dermatol. 19:37-43.
  9. Clarke, P.G. and S. Clarke. 1995. Historic apoptosis. Nature 378:230.
  10. Cleary, M. L., S.D. Smith and J. Sklar. 1986 Cloning and structural analysis of cDNAs for bcl-2 and a hybrid bcl-2/immunoglobulin transcript resulting from the t(14;18) translocation. Cell 47:19-28.
  11. Godon, C., F.P. Cordelieres, D. Biard, N. Giocanti, F. Megnin-Chanet, J. Hall and V. Favaudon. 2008. PARP inhibition versus PARP-1 silencing: different outcomes in terms of single-strand break repair and radiation susceptibility. Nucleic Acids Res. 36:4454-4464.
  12. Harrington, H.A., L.K. Ho, S. Ghosh and K.C. Tung. 2008. Construction and analysis of a modular model of caspase activation in apoptosis. Theor. Biol. Med. Model. 5:1-15.
  13. Hatano, T., R. Edamatsu, M. Hiramatsu, A. Mori, Y. Fujita, T. Yasuhara, T. Yoshida and T. Okuda. 1989. Effects of the interaction of tannins with co-existing substances. VI. Effects of tannins and ralated polyphenols on superoxide anion radical, and on 1,1-diphenyl-2-picrylhydrazyl radical. Chem. Pharm. Bull. 37:2016-2021.
  14. Kim, H.J., K.H. Kim, S.H. Yeom, M.K. Kim, J.G. Shim, H.W. Lim and M.W. Lee. 2005. New diarylheptanoids from the barks of Alnus japonica Steudel, Chin. Chem. Lett. 16:1337-1340.
  15. Lee, S.J. 1966. Korea Folk Medicine. Seoul National University Publishing Center Press, Seoul, Korea. p. 40.
  16. Lim, H.W., M.K. Kim, H.J. Kim, J.G. Shim, G.H. Kim, H.K. Choi and M.W. Lee. 2004. Quantitative determination of diarylheptanoid compounds from Korean Alnus. Kor. J. Pharmacogn. 35:384-387.
  17. Muller, S., J.P. Briand, S. Barakat, J. Lagueux, G.G. Poirier, G. De Murcia and D.A. Isenberg. 1994. Autoantibodies reacting with poly(ADP-ribose) and with a zinc-finger functional domain of poly(ADP-ribose) polymerase involved in the recognition of damaged DNA. Clin. Immunol. Immunopathol. 73:187-196.
  18. Oltvai, Z.N., C.L. Milliman and S.J. Korsmeyer. 1993. Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death. Cell 74:609-619.
  19. Re, R., N. Pellegrini, A. Proteggente, A. Pannala, M. Yang and C. Rice-Evans. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free. Radic. Biol. Med. 26:1231-1237.
  20. Schultz, N., E. Lopez, N. Saleh-Gohari and T. Helleday. 2003. Poly(ADP-ribose) polymerase (PARP-1) has a controlling role in homologous recombination. Nucleic Acids Res. 31:4959-4964.
  21. Sedlak, T.W., Z.N. Oltvai, E. Yang, K. Wang, L.H. Boise, C.B. Thompson and S.J. Korsmeyer. 1995. Multiple Bcl-2 family members demonstrate selective dimerizations with Bax. PNASU. 92:7834-7838.
  22. Tsujimoto, Y., L.R. Finger, J. Yunis, P.C. Nowell and C.M. Croce. 1984. Cloning of the chromosome breakpoint of neoplastic B cells with the t(14;18) chromosome translocation. Science 226:1097-1099.
  23. Xie, G.H., S.E. Choi, M.J. Mun, J.H. Jeong and K.H. Park. 2018. Ameliorative effects of combinative injection of Ginko biloba leaves extract and vitamin C on ischemia/reperfusion liver damages model. Korean J. Plant Res. 31:268-273.
  24. Zhap, Z.Q., J.M. Budde, C. Morris, N.P. Wang, D.A. Velez, S. Murak, R.A. Guyton and J.J. Vinten. 2001. Adenosine attenuated reperfusion induced apoptotic cell death by modulation expression of Bcl-2 and Bax proteins. J. Mol. Cell Cardiol. 33:57-68.