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Anti-allergic Effect of Eckolona cava Ethyl Acetate Fraction of on IgE/BSA-stimulated Bone Marrow-derived Cultured Mast Cells

IgE/BSA가 자극한 골수유래 비만 세포에 대한 감태 Ethyl Acetate 분획물의 항알러지 효능

  • Han, Eui Jeong (Department of Food Technology and Nutrition, Chonnam National University) ;
  • Kim, Hyun Soo (Department of Marine Life Science, Jeju National University) ;
  • Shin, Eun Ji (Department of Food Technology and Nutrition, Chonnam National University) ;
  • Kim, Min Ju (Department of Food Technology and Nutrition, Chonnam National University) ;
  • Han, Hee-Jin (Department of Food Technology and Nutrition, Chonnam National University) ;
  • Jeon, You-Jin (Department of Marine Life Science, Jeju National University) ;
  • Jee, Youngheun (Department of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University) ;
  • Ahn, Ginnae (Department of Food Technology and Nutrition, Chonnam National University)
  • 한의정 (전남대학교 식품공학.영양학과) ;
  • 김현수 (제주대학교 해양생명과학과) ;
  • 신은지 (전남대학교 식품공학.영양학과) ;
  • 김민주 (전남대학교 식품공학.영양학과) ;
  • 한희진 (전남대학교 식품공학.영양학과) ;
  • 전유진 (제주대학교 해양생명과학과) ;
  • 지영흔 (제주대학교 수의학과) ;
  • 안긴내 (전남대학교 식품공학.영양학과)
  • Received : 2018.12.06
  • Accepted : 2018.12.19
  • Published : 2018.12.30

Abstract

In this study, we investigated the anti-allergic effect of the ethyl acetate fraction of Ecklonia cava (EC-EtoAc) on the immunoglobulin E (IgE)/bovine serum albumin (BSA)-mediated activation of bone marrow-derived cultured mast cells (BMCMCs). We revealed that the $62.5{\mu}g/ml$ of EC-fractions ($EC-CHCl_3$, EC-Hexane and EC-EtoAc) inhibited IgE/BSA-activated ${\beta}$-hexosaminidase release from BMCMCs without cytotoxicity. Especially, EC-EtoAc showed the higher ${\beta}$-hexosaminidase release than the others. Also, EC-EtoAc reduced the expression levels of cytokines such as interleukin (IL)-$1{\beta}$, IL-4, IL-5, IL-6, IL-10, IL-13, interferon (IFN)-${\gamma}$ and tumor necrosis factor (TNF)-${\alpha}$ and a chemokine, thymus- and activation-regulated chemokine (TARC), compared to the only IgE/BSA-treated BMCMCs. Furthermore, EC-EtoAc significantly prevented the binding of IgE to Fc epsilon receptor $(Fc{\varepsilon}R)I$ and reduced the $Fc{\varepsilon}RI$ expression on the sensitized BMCMCs. Taken together, these results suggest that E. cava may be the natural agent with beneficial potentials for the treatment of type I allergic diseases induced by mast cell activation.

본 연구에서는 제주 자생해조류인 감태로부터 제조한 ethyl acetate 분획물의 비만 세포에 대한 항알러지 활성에 관한 연구를 수행하였다. IgE/BSA로 활성화시킨 비만 세포에서 EC-EtoAc는 세포 독성 없이 비만 세포의 탈과립 지표로 사용되는 ${\beta}$-hexosaminidase의 분비 뿐만 아니라 알러지 유발성 케모카인(TARC)과 사이토카인(IL-$1{\beta}$, IL-4, IL-6, IL-10, IL-13, IFN-${\gamma}$ 및 TNF-${\alpha}$)의 mRNA 발현을 억제하였다. 또한, EC-EtoAc는 비만 세포의 표면 리셉터인 $Fc{\varepsilon}RI$의 발현 및 IgE와 $Fc{\varepsilon}RI$의 결합능을 감소시켰다. 이 모든 결과로부터, 감태 유래 분획물인 EC-EtoAc가 활성화된 비만 세포에서 $Fc{\varepsilon}RI$의 발현 및 IgE와 $Fc{\varepsilon}RI$의 결합능을 감소시킴으로써 알러지 유발성 매개 인자들의 분비와 발현 등을 감소시켜 항알러지 효능을 가진다는 것을 알 수 있었다. 또한, 이 연구 결과는 EC-EtoAc가 항알러지 효능을 보이는 천연 소재로서 이용가치가 있다는 것을 제시한다.

Keywords

Acknowledgement

Supported by : 한국해양과학기술진흥원

References

  1. Kim, Y. J., Park, S. J., and Kim, T. J.: Anti-allergic effects of nodakenin in IgE/Ag-induced type I hypersensitivity. J. Life Sci. 2011, 21, 1721-1725. https://doi.org/10.5352/JLS.2011.21.12.1721
  2. Lee, J. H., Kim, J. H., Park, S. Y., and Choi, J. H.: Effects of Curcuma longa Rhizoma on asthma induced intra-nasal instillation of ovalbumin in mice. J. Korean Med. Ophthalmol. Otolaryngol. Dermatol. 2008, 21, 20-35.
  3. Kim, H. G.: The roles of mast cells in allergic inflammation and mast cell-related disorders. Allergy Asthma Respir. Dis. 2017, 5, 248-255. https://doi.org/10.4168/aard.2017.5.5.248
  4. Jung, M. K.: Role of inflammatory mediators in the pathogenesis of otitis media. Korean J. Otolaryngol-Head Neck Surg. 2007, 50, 2-13.
  5. Schwartz, L. B., Austen, K. F., and Wasserman, S. I.: Immunologic release of ${\beta}$-hexosaminidase and ${\beta}$-glucuronidase from purified rat serosal mast cells. J. Immunol. 1979, 123, 1445-1450.
  6. Yoon, T. J., Lee, S. W., Shin, K. S., Choi, W. H., Hwang, S. H., Seo, S. H., and Park, W. M.: Effect of hot water extract from Acanthopanax senticosus on systemic anaphylaxis. Korean J. Food Sci. Technol. 2002, 34, 518-523.
  7. Kim, Y. T., Lee, J. H., Ko, J. Y., Oh, J. Y., Sok, C. H., Hong, J. T., and Jeon, Y. J.: Isolation of eckol from Ecklonia cava via centrifugal partition chromatography (CPC) and characterization of it's anti-inflammatory activity. Korean J. Fish Aquat. Sci. 2015, 48, 301-307.
  8. Wijesekara, I., Yoon, N. Y., and Kim, S. K.: Phlorotannins from Ecklonia cava (phaeophyceae): Biological activities and potential health benefits. Biofactors 2010, 36, 408-414. https://doi.org/10.1002/biof.114
  9. Matsuda, A., Tanaka, A., Pan, W., Okamoto, N., Oida, K., Kingyo, N., and Kajiwara, N.: Supplementation of the fermented soy product ImmuBalance TM effectively reduces itching behavior of atopic NC/Tnd mice. J. Dermatol. Sci. 2012, 67, 130-139. https://doi.org/10.1016/j.jdermsci.2012.05.011
  10. Do, G. P., Lee, H. J., Do, J. R., and Kim, H. K.: Antiobesity effect of the Cucubita moschata duch extracts in 3T3-L1 adipocyets. Korean J. Food Preserv. 2012, 19, 138-143. https://doi.org/10.11002/kjfp.2012.19.1.138
  11. Kim, Y. M.: Inhibitory effect of Penthorun chinense extract on allergic responses in vitro and in vivo. J. Food Hyg. Saf. 2015, 30, 376-382. https://doi.org/10.13103/JFHS.2015.30.4.376
  12. Lee, S. Y.: The antiallergic and antioxidative effects of Chaenomeles Sinensis (CS) in RBL 2H3 cells. Korean J. Pediatr. 2010, 24, 126-136.
  13. Park, Y. L. and Park, J.: Pathogenesis of atopic dermatitis. Korean J. Invest. Dermatol. 2007, 14, 67-72.
  14. Kim, S. W., Cho, J. S., Park, J. K., Lee, K. H., and Cha, C. I.:Expression of cytokines in coculture model with epithelial cells and fibroblasts of nasal polyp. Korean J. Otolaryngol- Head Neck Surg. 2017, 50, 134-138.
  15. Lee, S. J. and Kim, Y. B.: The effects of coisis semen extract on blood serum cytokine of allergic rhinitis mice model. J. Korean Med. Ophthalmol. Otolaryngol. Dermatol. 2013, 26, 58-67. https://doi.org/10.6114/jkood.2013.26.2.058
  16. Kwon, M. H., Lee, J. Y., and Kim, D. G.: Anti-allergic and anti-inflammatory effects of jacho (Lithospermum Erythrorhizon). J. Korean Oriental. Med. 2005, 26, 110-123.
  17. Seo, U. K., Lee, J. I., Park, J. H., and Park, Y. K.: The ethylacetate extract of north kangwhal (Ostericum koreanum) attenuates the inflammatory responses in PMA/A23187-stimulated mast cells. Korean J. Herbol. 2018, 23, 81-89.
  18. Park, J. H. and Kim, K. J.: The effect of Moutan Cortex on pro-inflammatory cytokines through NF-${\kappa}B$ & MAPKs pathway in HMC-l. J. Korean Med. Ophthalmol. Otolaryngol. Dermatol. 2009, 22, 1-18.
  19. Ahn, K.: Role of mast cells in allergic inflammation and innate immunity. Korean J. Pediatr. 2004, 47, 1137-1141.