Reproductive and Developmental Biology

  • 제42권2호
  • /
  • Pages.7-12
  • /
  • 2018
  • /
  • 1738-2432(pISSN)
  • /
  • 2288-0151(eISSN)
한국동물번식학회 (The Korean Society of Animal Reproduction)
권호 표지
DOI QR코드

DOI QR Code

Biochemical Characterization of 20α-Hydroxysteroid Dehydrogenase

  • Byambaragchaa, Munkhzaya (Animal Biotechnology, Graduate School of Future Convergence Technology, Department of Animal Life Science, Institute of Genetic Engineering, Hankyong National University) ;
  • Min, Kwan-Sik (Animal Biotechnology, Graduate School of Future Convergence Technology, Department of Animal Life Science, Institute of Genetic Engineering, Hankyong National University)
  • 발행 : 2018.05.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

In this review, we have tried to summarize the evidence and molecular characterization indicating that $20{\alpha}$-hydroxysteroid dehydrogenase ($20{\alpha}$-HSD) is a group of the aldo-keto reductase (AKR) family, and it plays roles in the modulation and regulation of steroid hormones. This enzyme plays a critical role in the regulation of luteal function in female mammals. We have studied the molecular expression and regulation of $20{\alpha}$-HSD in cows, pigs, deer, and monkeys. The specific antibody against bovine $20{\alpha}$-HSD was generated in a rabbit immunized with the purified recombinant protein. The mRNA expression levels increased gradually throughout the estrous cycle, the highest being in the corpus luteum (CL) 1 stage. The mRNA was also specifically detected in the placental and ovarian tissues during pregnancy. The $20{\alpha}$-HSD protein was intensively localized in the large luteal cells and placental cytotrophoblast villus, glandular epithelial cells of the endometrium, syncytiotrophoblast of the placenta, the isthmus cells of the oviduct, and the basal part of the primary chorionic villi and chorionic stem villus of the placenta and large luteal cells of the CL in many mammalian species. Further studies are needed to determine the functional significance of the $20{\alpha}$-HSD molecule during ovulation, pregnancy, and parturition. This article will review how fundamental information of these enzymes can be exploited for a better understanding of the reproductive organs during ovulation and pregnancy.

키워드

참고문헌

  1. Albarracin CT, Parmer TG, Duan WR, Nelson SE, Gibori G (1994): Identification of a major prolactinregulated protein as $20{\alpha}$-HSD: coordinate regulation of its activity, protein content, and messenger ribonucleic acid expression. Endocrinology 134:2453-2460. https://doi.org/10.1210/endo.134.6.8194472
  2. Bao L, Tessier C, Prigent-Tessier A, Li F, Buzzio OL, Callegari EA, Horseman ND, Gibori G (2007): Decidual prolactin silences the expression of genes detrimental to pregnancy. Endocrinology 148:2326-2334. https://doi.org/10.1210/en.2006-1643
  3. El-Kabbani O, Dhagat U, Hara A (2011): Inhibitors of human $20{\alpha}$-hydroxysteroid dehydrogenase (AKR1C1). J Steroid Biochem and Mol Bio 125:105-111. https://doi.org/10.1016/j.jsbmb.2010.10.006
  4. Higaki Y, Kamiya T, Usami N, Shintani S, Shiraishi H, Ishikura S, Yamamoto I, Hara A (2002): Molecular characterization of two monkey dihydrodiol dehydrogenases. Drug Metab Pharmacokinet 17:348-356. https://doi.org/10.2133/dmpk.17.348
  5. Hirabayashi K, Ishida M, Suzuki M, Yamanouchi K, Nishihara M (2004): Characterization and functional analysis of the 5'-flanking region of the mouse $20{\alpha}$-hydroxysteroid dehydrogenase gene. Biochem J 382:975-980. https://doi.org/10.1042/BJ20040276
  6. Ishida M, Chang K, Hirabayashi K, Nishirara M, Takahashi M (1999): Cloning of mouse $20{\alpha}$-hydroxysteroid dehydrogenase cDNA and its mRNA localization during pregnancy. J Reprod Dev 45:321-329. https://doi.org/10.1262/jrd.45.321
  7. Jayasekara WS, Yonezawa T, Ishita M, Yamanouchi K, Nishihara M (2004): Molecular cloning of goat $20{\alpha}$-hydroxysteroid dehydrogenase cDNA. J Reprod Dev 50:323-331. https://doi.org/10.1262/jrd.50.323
  8. Jayasekara WS, Yonezawa T, Ishita M, Yamanouchi K, Nishihara M (2005): Expression and possible role of $20{\alpha}$-hydroxysteroid dehydrogenase in the placenta of the goat. J Reprod Dev 51:265-272. https://doi.org/10.1262/jrd.16074
  9. Jez JM, Bennett MJ, Schlegel BP, Lewis M, Penning TM (1997): Comparative anatomy of the aldoketo reductase superfamily. Biochem J 326:625-636. https://doi.org/10.1042/bj3260625
  10. Kim SH, Shin YS, Kang MH, Yoon JT, Min KS (2014): Gene expression and localization of $20{\alpha}$-HSD in reproductive tissues during early pregnancy of cattle. Anim Reprod Sci 147:1-9. https://doi.org/10.1016/j.anireprosci.2014.03.016
  11. Liu H, Bellemare V, Labrie F, Luu-The V (2007):Molecular characterization of the cynomolgus monkey Macacafascicularis steroidogenic enzymes belonging to the aldo-keto reductase family. J Steroid Biochem Mol Biol 104:75-80. https://doi.org/10.1016/j.jsbmb.2006.10.004
  12. Meyer HH (1994): Luteal versus placental progesterone:the situation in the cow, pig and bitch. Exp Clin Endocrinol 102:190-192.
  13. Miura R, Shiota K, Noda K, Yagi S, Ogawa T, Takahashi M (1994): Molecular cloning of cDNA for rat ovarian $20{\alpha}$-hydroxysteroid dehydrogenase (HSD1). Biochemistry J 15:561-567.
  14. Naidansuren P, Park CW, Kim SH, Nanjidsuren T, Park JJ, Yun SJ, Sim BW, Hwang SS, Kang MH, Ryu BY, Hwang SY, Yoon JT, Yamanouchi K, Min KS (2011): Molecular characterization of bovine placental and ovarian $20{\alpha}$-HSD. Reproduction 142:723-731. https://doi.org/10.1530/REP-11-0093
  15. Naidansuren P, Park CW, Nanjidsuren T, Park JJ, Yun SJ, Kang MH, Yamanouchi K, Min KS (2012): Ovarian and placental of $20{\alpha}$-HSD during pregnancy in deer. Anim Reprod Sci 130:63-73. https://doi.org/10.1016/j.anireprosci.2011.12.016
  16. Nanjidsuren T, Min KS (2014): The transcription factor Ap-1 regulates monkey $20{\alpha}$-hydroxysteroid dehydrogenase promoter activity in CHO cells. BMC Biotechnology 14:71. https://doi.org/10.1186/1472-6750-14-71
  17. Nanjidsuren T, Naidansure P, Park CW, Park JJ, Yun SJ, Sim BW, Kang MH, Lee SR, Chang KT, Min KS (2011): Expression and localization of the $20{\alpha}$-hudroxysteroid dehydrogenase enzyme in the reproductive tissues of the cynomolgus monkey Macaca fascicularis. J Steroid Biochem Mol Biol 127:337-344. https://doi.org/10.1016/j.jsbmb.2011.07.006
  18. Nanjidsuren T, Yun SJ, Park CW, Kim MS, Kang MH, Min KS (2014): Expression and localization of $20{\alpha}$-HSD in porcine reproductive tissues during pregnancy. Anim Reprod Sci 148:63-71. https://doi.org/10.1016/j.anireprosci.2014.04.015
  19. Nishizawa M, Nakajima T, Yasuda K, Kanzaki H, Sasaguri Y, Watanabe K, Ito S (2000): Close kinship of human $20{\alpha}$-hydroxysteroid dehydrogenase gene with three aldo-keto reductase genes. Genes Cells 5:111-125. https://doi.org/10.1046/j.1365-2443.2000.00310.x
  20. Pallai R, Simpkins H, Chen J, Parekh HK (2010):The CCAT box binding transcription factor, nuclear factor-Y (NF-Y) regulates, transcription of human aldo-keto reductase 1C1 gene. Gene 549:11-23.
  21. Park CW, Jeong SK, Nanjidsuren T, Byambaragchaa M, Kang MH, Sim BW, Min KS (2018): Characterization of transgenic mice expressing EGFP under the control of the monkey $20{\alpha}$-hydroxysteroid dehydrogenase promoter. Sci China Life Sci DOI https://doi.org/10.1007/s11427-017-9111-9.
  22. PenningTM (2011): Human hydroxysteroid dehydrogenases and pre-receptor regulaltion: Insights into inhibitors design and evaluation. J Steroid Biochem Mole Biol 125:46-56. https://doi.org/10.1016/j.jsbmb.2011.01.009
  23. Rinzer TL, Smuc T, Rupreht R, Sinkovec J, Penning TM (2006): AKR1C1 and AKR1C3 may determine progesterone and estrogen ratios in endometrial cancer. Mol Cell Endocrinol 248:126-135. https://doi.org/10.1016/j.mce.2005.10.009
  24. Schuler G, Teichmann U, Kowalewski MP, Hoffmann B, Madore E, Fortier MA, Klisch K (2006): Expression of cyclooxygenase-II (COX-II) and 20alpha-hydroxysteroid dehydrogenase/prostaglandin Fsynthesis in bovine placentomes: implications for the initiation of parturition in cattle. Placenta 27:1022-1029. https://doi.org/10.1016/j.placenta.2005.11.001
  25. Selga E, Noe V, Ciudad CJ (2008): Transcriptional regulation of aldo-keto reductase 1C1 in HT29 human colon cancer cells resistant to methotrexate: role in the cell cycle and apoptosis. Biochem Pharmacol 75:414-426. https://doi.org/10.1016/j.bcp.2007.08.034
  26. Seo KS, Naidansuren P, Kim SH, Yun SJ, Park JJ, Sim BW, Park CW, Nanjidsuren T, Kang MH, Seo HW, Ka HK, Kim NH, Hwang SY, Yoon JT, Yamanouchi K, Min KS (2011): Expression of aldo-keto reductase family 1 member C1 (AKR1C1) gene in porcine ovary and uterine endometrium during the estrous cycle and pregnancy. Reprod Biol Endocrinol 9:e139. https://doi.org/10.1186/1477-7827-9-139
  27. Seong HH, Min KS, Kang MH, Yoon JT, Jin HJ, Chung HJ, Chang WK, Yun SG, Shiota K (2002): Change in ovarian and placental $20{\alpha}$-HSD activity during the pregnancy in the rat. Asian-Aust J Anim Sci 16:342-347.
  28. Seong HK, Seo KS, Kim JS, Her CG, Kang MH, Sim BW, Yoon JT, Min KS (2017): Studies on steroid hormone concentration during the estrous cycle in the medikinetics micropig. Reprod Dev Bio 41:1-6. https://doi.org/10.12749/RDB.2017.41.1.1
  29. Shiota K, Seong HH, Noda K, Hattori N, Ikeda A, Ogura A, Itagaki S, Takahashi M, Ogawa T (1993):$20{\alpha}$-HSD activity in rat placenta. Endocrine J 40:673-681. https://doi.org/10.1507/endocrj.40.673
  30. Stocco CO, Lau LF, Gibori G (2002): A calcium/calmodulin-dependent activation of ERK1/2 mediates Jun D phosphorylation and induction of nur77 and $20{\alpha}$-HSD genes by prostaglandin $F2{\alpha}$ in ovarian cells. J Biol Chem 277:3293-3302. https://doi.org/10.1074/jbc.M110936200
  31. Stocco CO, Zhong L, Sugimoto Y, Ichikawa A, Lau LF, Gibori G (2000): Prostaglandin $F2{\alpha}$-induced expression of $20{\alpha}$-hydroxysteroid dehydrogenase involves the transcription factor NUR77. J Biol Chem 275:37202-37211. https://doi.org/10.1074/jbc.M006016200
  32. Tsumagari S, Kamata J, Takagi K, Tanemura K, Yoshi A, Takeishi M (1994): 3 beta-hydroxysteroid dehydrogenase activity and gestagen concentrations in bovine cotyledons and caruncles during gestation and parturition. J Reprod Fetil 102:35-39. https://doi.org/10.1530/jrf.0.1020035
  33. Zhang Y, Dufort I, Rheault P, Luu-The V (2000):Characterization of a human $20{\alpha}$-HSD. J Mol Endocrinol 25:221-228.
  34. Zhong L, Ou J, Barkai U, Mao J, Frasor J, Gibori G. (1998): Molecular cloning and characterization of the rat ovarian 20alpha-hydroxysteroid dehydrogenase gene. BBRC 249:797-803.