Journal of Microbiology and Biotechnology

  • 제28권7호
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  • Pages.1141-1146
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  • 2018
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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A Simple Enzymatic Method for Quantitation of 2'-Fucosyllactose

  • Seydametova, Emine (Department of Integrative Biotechnology, College of Biotechnology and Bioengineering, Sungkyunkwan University) ;
  • Shin, Jonghyeok (Department of Integrative Biotechnology, College of Biotechnology and Bioengineering, Sungkyunkwan University) ;
  • Yu, Jiwon (Department of Integrative Biotechnology, College of Biotechnology and Bioengineering, Sungkyunkwan University) ;
  • Kweon, Dae-Hyuk (Department of Integrative Biotechnology, College of Biotechnology and Bioengineering, Sungkyunkwan University)
  • 투고 : 2018.03.07
  • 심사 : 2018.05.01
  • 발행 : 2018.07.28
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초록

2'-Fucosyllactose (2'-FL) is one of the most important human milk oligosaccharides and has several health benefits for infants. The levels of 2'-FL in breast milk or samples from other sources can be quantified by high-performance liquid chromatography. However, this method cannot be used for simultaneous detection of the target compound in numerous samples. Here, we developed a simple method for quantifying 2'-FL in a microplate format. The method involves two steps: (i) release of $\text\tiny{L}$-fucose from 2'-FL by ${\alpha}$-(1-2,3,4,6)-$\text\tiny{L}$-fucosidase and (ii) measurement of NADPH formed during the oxidation of $\text\tiny{L}$-fucose by $\text\tiny{L}$-fucose dehydrogenase. This method enables measurement of up to 5 g/l 2'-FL in 50 min using a 96-well microplate. The efficiency and simplicity of the proposed method make it suitable for the analyses of a large number of samples simultaneously.

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참고문헌

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피인용 문헌

  1. Development of fluorescent Escherichia coli for a whole-cell sensor of 2ʹ-fucosyllactose vol.10, pp.None, 2020, https://doi.org/10.1038/s41598-020-67359-x