Journal of Microbiology and Biotechnology

  • 제28권7호
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  • Pages.1078-1085
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  • 2018
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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A New Salt-Tolerant Thermostable Cellulase from a Marine Bacillus sp. Strain

  • dos Santos, Yago Queiroz (Laboratorio de Quimica e Funcao de Proteinas Bioativas, Universidade Federal do Rio Grande do Norte) ;
  • de Veras, Bruno Oliveira (Departamento de Biologia Celular e Molecular, Universidade Federal da Paraiba) ;
  • de Franca, Anderson Felipe Jacome (Laboratorio de Quimica e Funcao de Proteinas Bioativas, Universidade Federal do Rio Grande do Norte) ;
  • Gorlach-Lira, Krystyna (Departamento de Biologia Celular e Molecular, Universidade Federal da Paraiba) ;
  • Velasques, Jannaina (Centro de Formacao em Ciencias e Tecnologias Agroflorestais, Universidade Federal do Sul da Bahia) ;
  • Migliolo, Ludovico (Laboratorio de Quimica e Funcao de Proteinas Bioativas, Universidade Federal do Rio Grande do Norte) ;
  • dos Santos, Elizeu Antunes (Laboratorio de Quimica e Funcao de Proteinas Bioativas, Universidade Federal do Rio Grande do Norte)
  • 투고 : 2018.02.23
  • 심사 : 2018.04.26
  • 발행 : 2018.07.28
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초록

A salt-tolerant cellulase secreted by a marine Bacillus sp. SR22 strain with wide resistance to temperature and pH was purified and characterized. Its approximate mass was 37 kDa. The endoglucanase, named as Bc22Cel, was purified by ammonium sulfate precipitation, gel filtration chromatography, and extraction from the gel after non-reducing sodium dodecyl sufate-polyacrylamide gel electrophoresis. The optimal pH value and temperature of Bc22Cel were 6.5 and $60^{\circ}C$, respectively. The purified Bc22Cel showed a considerable halophilic property, being able to maintain more than 70% of residual activity even when pre-incubated with 1.5 M NaCl for 1 h. Kinetic analysis of the purified enzyme showed the $K_m$ and $V_{max}$ to be 0.704 mg/ml and $29.85{\mu}mol{\cdot}ml^{-1}{\cdot}min^{-1}$, respectively. Taken together, the present data indicate Bc22Cel as a potential and useful candidate for industrial applications, such as the bioconversion of sugarcane bagasse to its derivatives.

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