The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Study on the Protective Mechanism of Vitamin C in the SH-SY5Y Cell Death Induced by the Streptozotocin

스트렙토조토신으로 유도된 신경세포사멸에서 비타민 C의 보호 기전 연구

  • 이승희 (서울의료원 의학연구소) ;
  • 한경훈 (서울의료원 의학연구소) ;
  • 김현준 (서울의료원 의학연구소) ;
  • 박광성 (서울의료원 의학연구소) ;
  • 한성희 (고려대학교 안암병원 의생명연구센터) ;
  • 김정희 (서울의료원 신경외과) ;
  • 허재혁 (서울의료원 신경과)
  • Received : 2018.03.24
  • Accepted : 2018.07.09
  • Published : 2018.08.31
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Abstract

In this study, we analyzed the protective effects of the vitamin C in the streptozotocin (STZ)-induced apoptosis using the SH-SY5Y, a neuroblastoma cell line. The cells were pretreated with the vitamin C ($100{\mu}g$) for 30 min, followed by the 24-hr treatment with the 2.5-mM STZ. The cell-viability assay using the Cell Counting Kit (CCK)-8 revealed the cell-survival rate increased by 15% following the vitamin-C pretreatment compared to the STZ-only treatment. Moreover, we conducted the western-blot analysis to determine the protective effect of the vitamin C regarding the apoptosis. Compared to those in the STZ-only-treatment group, the p-ERK and Bcl-2 expressions increased in the vitamin-C-pretreatment group, whereas the p-JNK and Bax expressions decreased. The vitamin-C pretreatment increased the expression of the SOD-1, an antioxidant enzyme, by more than 30%, indicating its protective role in the STZ-induced oxidative stress. Also, we found both the intrinsic- and extrinsic-pathway mechanisms of the STZ-induced apoptosis. The results of this study $s{\mu}ggest$ vitamin C may help prevent the neurodegenerative diseases.

Keywords

References

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