Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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Effects of Heavy Metals on Mortality and Metabolite Changes in Mosquitoes, Culex pipiens pallens (Diptera: Culicidae)

중금속이 빨간집모기(Culex pipiens)의 치사율과 대사물질의 변화에 미치는 영향

  • Lee, Su Mi (Department of Biology, Changwon National University) ;
  • Shin, Byung Sik (Department of Biology, Changwon National University)
  • 이수미 (국립 창원대학교 생물학과) ;
  • 신병식 (국립 창원대학교 생물학과)
  • Received : 2018.12.21
  • Accepted : 2018.12.24
  • Published : 2018.12.31
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Abstract

To investigate the effect of heavy metals (Cu, Cd, Hg, Pb, Zn) on mosquito Culex pipiens, 50% lethal concentration ($LC_{50}$) concentration, total lipid content, lipid content composition, and total protein content were measured. The results showed that the Hg $LC_{50}$ was $0.45mg\;kg^{-1}$ and the Hg toxicity was higher than the other metals. The results also showed that cadmium (Cd) significantly retarded the growth of mosquito larvae among the tested heavy metals. Six types of lipid bands were isolated from mosquito samples exposed to heavy metals, and five of them were identified (phospholipid, cholesterol, fatty acid, triglyceride, and cholesterol ester). The total lipid content of all treatments decreased compared to the control. In addition, the protein content of the control group ($0.51mg\;ind.^{-1}$) was higher than that of the treated group, and the protein content of the larvae treated with Hg and Cd was very low. In conclusion, heavy metals not only lower the survival rate of mosquitoes, but also affect the protein content and lipids in the mosquito's body, thereby causing growth inhibition. These results indicate that heavy metal contamination will affect the size of mosquito population by inhibiting the survival and growth of mosquitoes, and it is expected that it can be used to measure the degree of contamination using mosquito through subsequent studies.

중금속이 곤충에 미치는 영향을 조사하기 위하여 빨간집모기(Culex pipiens pallens) 금속용액(Cu, Cd, Hg, Pb, Zn)에서 사육한 후 50% 치사농도($LC_{50}$) 용화시기, 총 지질함량, 지질조성, 총 단백질 함량변화를 측정하였다. 50% 치사농도($LC_{50}$)는 3령 유충으로 24시간 처리한 Hg 처리군에서 $0.45mg\;kg^{-1}$으로 나타나 다른 금속에 비해 독성이 강한 것으로 나타났고, 용화 시기는 처리군이 대조군(129시간)에 비해 지연되었으며 Cd가 처리군 중 273시간으로 가장 늦게 용화되었다. 중금속에 노출된 모기시료에서 총 여섯 종류의 지질 band가 분리되었으며, 이 중 5종의 지질이 동정되었다(phospholipid, cholesterol, fatty acid, triglyceride, cholesterol ester). 모든 처리군의 총 지질함량은 대조군에 비해 감소하였다. 또한, 대조군의 단백질 함량($0.51mg\;ind.^{-1}$)이 처리군의 단백질 함량에 비해 높게 나타났으며, 처리군 중 Hg과 Cd을 처리한 유충의 단백질 함량이 매우 낮게 나타났다. 결론적으로 중금속이 처리된 용액에서 사육된 모기유충은 오염된 환경에 의한 먹이 섭취의 장애 및 중금속의 독성으로 인해 단백질 함량과 지질의 감소가 일어나 발육이 느려지고 용화시기가 길어지는 것으로 생각되며 이는 중금속에 의한 체내 물질대사의 변화를 의미한다. 이러한 결과는 중금속 오염이 모기의 생존과 성장을 억제시켜 모기 개체군 크기에 영향을 줄 것을 나타내며, 추후 연구를 통해 모기의 생육정도를 이용한 오염도 측정에 활용이 가능할 것으로 기대된다.

Keywords

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