Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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Effect of Semisulcospira libertina Extract on Hepatic Injury Induced by D-galactosamine

다슬기 추출물이 D-galactosamine에 의해 손상된 간에 미치는 효과

  • Park, Young Mi (Department of Biological Science, Andong National University) ;
  • Lee, Jong Eun (Department of Biological Science, Andong National University) ;
  • Seo, Eul Won (Department of Biological Science, Andong National University)
  • 박영미 (안동대학교 자연과학대학 생명과학과) ;
  • 이종은 (안동대학교 자연과학대학 생명과학과) ;
  • 서을원 (안동대학교 자연과학대학 생명과학과)
  • Received : 2018.09.05
  • Accepted : 2018.11.12
  • Published : 2018.12.31
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Abstract

The purpose of this study is to examine the restorative effect of Semisulcospira libertina extract, on damaged liver cells induced by D-galactosamine in rats. Treatment of damaged liver cells with S. libertina extract significantly reduced local fatty degeneration, and inflammatory cell necrosis, to levels similar with the undamaged control group. In addition, S. libertina extracts were found to reduce plasma levels of liver damage indicator enzymes, such as AST, ALT, LDH and ALP, to control levels. It also reduced lipid peroxides, and lipid contents within damaged liver tissues. This suggests that S. libertina extract has a restorative effect on liver cells, thus reducing release of damage-associated liver enzymes, and oxidative degradation of lipids. Also, S. libertina extracts were found to be involved in recovery of damaged cells from inflammatory response by suppressing expression of $TNF-{\alpha}$, which leads to tissue injury and necrosis, whereas inducing expression of HO-1 that protects cells during inflammation. Thus, S. libertina extract restores liver tissue from necrosis and fibrosis, as well modulates expression of inflammation-related genes against liver damage. Our findings suggest that S. libertina extract is an effective medicinal resource, for improving and recovering liver cells from hepatic injury.

본 연구는 D-galactosamine에 의해 간 손상이 유도된 흰쥐에 다슬기(Semisulcospira libertina) 추출물이 손상된 간세포의 개선 및 회복에 미치는 효과에 관해 조사하였다. 다슬기 추출물은 간 손상에 따른 간 조직 내 국소적 지방 변성과 염증세포 침윤을 크게 감소시켜 대조군과 유사한 수준으로 회복시키는 경향을 나타내었다. 또한 다슬기 추출물은 간 손상 지표 효소인 AST와 ALT, LDH 및 ALP의 증가된 효소 활성을 대조군 수준으로 완화시키며, 간 조직 내 지질과 과산화지질의 함량을 감소시켜 간 손상으로 인한 혈중 효소 활성과 조직 내 지질 함량을 개선하는 것으로 조사되었다. 이와 더불어 다슬기 추출물은 염증반응을 촉진시켜 조직상해 및 괴사를 유도하는 $TNF-{\alpha}$의 발현을 억제시키며, 염증 시 세포를 보호하는 HO-1의 발현을 촉진시켜 염증 반응에서 손상된 세포를 대조군 상태의 세포로 회복시키는데 관여하는 것으로 조사되었다. 따라서 다슬기 추출물은 간조직의 괴사 및 섬유화를 회복시키고 혈액 내 효소의 활성과 조직 내 지질 함량을 개선할 뿐만 아니라 염증 반응 인자의 발현을 조절하고 있어 간 손상으로 인한 간세포의 개선 및 회복에 효과가 높은 기능성 소재로서의 가능성을 제시해 주고있다.

Keywords

References

  1. Bligh EG and WJ Dyer. 1959. A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37:911-917. https://doi.org/10.1139/o59-099
  2. Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
  3. Cherkasov VS and NS Nemchenko. 1978. Determination of the activity of blood serum alanine- and aspartate aminotrans-ferases (ALT, AST) in various forms of hearing disorders. Zh Ushn Nos Gorl Bolezn. 23:220-228.
  4. Cho KH, HJ Choo, MG Seo, JC Kim, YJ Shin, GH Ryu, HY Cho, CY Jeong and YS Hah. 2017. Effect of Semisulcospira libertina extracts from different extraction processes on liver cell toxicity and ethanol metabolism. Food Eng. Prog. 21:158-166. https://doi.org/10.13050/foodengprog.2017.21.2.158
  5. Cubero FJ and N Nieto. 2008. Ethanol and arachidonic acid synergize to activate Kupffer cells and modulate the fibrogenic response via tumor necrosis factor alpha, reduced glutathione, and transforming growth factor beta-dependent mechanisms. Hepatology 48:2027-2035.
  6. Eu JB, SO Kim, TJ Seoung, SG Choi, SH Cho and CY Choi. 2010. Protective effect of theanine on the acetaminophen-induced hepatotoxicity. J. Korean Soc. Food Sci. Nutr. 39:350-355.
  7. Folch J, L Mee and GSH Stanley. 1975. A simple method for the isolation and purification of total lipid from animal tissues. J. Biol. Chem. 226:497-509.
  8. Hong SS, GT Gibney, M Esquilin, J Yu and Y Xia. 2004. Effect of protein kinase on lactate dehydrogenase activity in cortical neurons during hypoxia. Brain res. 1009:195-202. https://doi.org/10.1016/j.brainres.2004.03.004
  9. Immenschuh S, E Baumgart-Vogt and S Mueller. 2010. Heme oxygenase-1 and iron in liver inflammation: a complex alliance. Curr. Drug Targets 11:1541-1550.
  10. Jeon TW, ES Lee, YS Lee, OK Ham, MH Park, KJ Kim and HJ Kim. 2002. Hepatoprotective effects of Semisulcospira libertina and garlic on the liver damage induced by carbon tetrachloride in rats. J. Korean Soc. Food Sci. Nutr. 31:516-520.
  11. Jung HC, JS Sin, EJ Kim, SH Shin, JY Jang, KS Shin, YB Kim, JK Kang and SY Hwang. 2004. Protective effect of Lycii fructus extract against Hepatotoxicity induced by carbon tetrachloride. Korean J. Lab. Ani. Sci. 20:187-193.
  12. Kim OK. 2001. Protective effects of extracts of Diospyrus kaki folium against hepatotoxicity in carbon tetrachloride intoxicated rat. J. Korean Soc. Food Sci. Nutr. 30:97-101.
  13. Kim YK, HS Moon, MH Lee, MJ Park, CW Lim, HY Park, JI Park, HD Yoon and DH Kim. 2009. Biological activities of seven melania snails in Korea. Kor. J. Fish. Aquat. Sci. 42:434-441.
  14. Kim YH, TK Lee and YS Cha. 1985. Studies on the nutritive component of black snail (Semisulcospira libertina). Bull. Agric. College Chonbuk Univ. 16:101-105.
  15. Kind PR and EJ King. 1954. Estimation of plasma phosphatase by determination of hydrolysed phenol with amino antipyrine. J. Clin. Pathol. 7:322-326.
  16. Lee IW, HS Choi and SM Kim. 2011. Hepatoprotective activity of Spatholobi caulis water extract against acetaminophen- induced toxicity in rats. Korea J. Herbol. 26:65-73.
  17. Lee JH, SC Chi, SH Kim, YH Shin and JW Choi. 2005. Protective effect of DWP-04 against hepatotoxicity induced by D-galactosamine. J. Life Sci. 15:461-467.
  18. Lee MH, YK Kim, HS Moon, YA Kim, NY Yoon, CW Lim, HY Park and DH Kim. 2010. Antioxidant activities of five melania snails of the genus Semisulcospira in Korea. Kor. J. Fish. Aquat. Sci. 43:188-194.
  19. Lee TJ and KJ Min. 2010. The effect of Allium sativum L. extract on hepatic function if rats with CCl4-induced (hepatic) injury. J. Kor. Acad. Industr. Coop. Soc. 11:1936-1942.
  20. Lee UE and SL Friedman. 2011. Mechanisms of hepatic fibrogenesis. Best Pract. Res. Clin. Gastroenterol. 25:195-206.
  21. Lee WC, HA Jung, JS Cjoi, YS Kim and SM Lee. 2011. Protective effect of luteolin against apoptotic liver damage induced by D-galactosamine/lipopolysaccharide in mice. J. Nat. Prod. 74:1916-1921.
  22. Lee YM, JJ Lee, HD Shin and MY Lee. 2006. Protective effects of Chaenomeles sinensis Koehne extract on ethanol-induced liver damage in rat. J. Korean Soc. Food Sci. Nutr. 35:1336-1342.
  23. Lee YS, OK Han, TW Jeon, ES Lee, KJ Kim, CW Park and HJ Kim. 2002. Effect of Astragali radix extract on acetaminophen-induced hepatotoxicity in mice. J. Physiol. Pathol. Korean Med. 16:707-713.
  24. Lesch R, W Reutter, D Keppler and K Decker. 1969. Liver restitution after acute galactosaminee hepatitis: autoradiographic and biochemical studies in rats. Exp. Mol. Pathol. 12:58-69.
  25. Maley F, AL Tarentino, JF McGarrahan and R Giacco. 1968. The metabolism of D-galactosamine and N-acetyl-D-galactosamine in rat liver. Biochem. J. 107:637-644.
  26. Oh SY, JS Lee, SH Seo, TS Kim and JY Ma. 2011. Paeonia lactiflora Pall prevents $H_2O_2$-induced hepatotoxicity by increasing HSP72 and HO-1. J. Physiol. Pathol. Korean Med. 25:843-848.
  27. Ohkawa H, N Ohishi and K Yagi. 1979. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 95:351-358.
  28. Park JY, CM Park, JJ Kim and YS Song. 2008. Hepatoprotective activity of dandelion (Taraxacum officinale) water extract against D-galactosamine-induced hepatitis in rats. J. Korean Soc. Food Sci. Nutr. 37:177-183.
  29. Park YM, JA Kim, CH Kim, JH Lim and EW Seo. 2015. Effect of cultured Acer tegmentosum cell extract against hepatic injury induced by D-galactosamine in SD-rats. Korean J. Plant Res. 28:551-560.
  30. Park YM, JH Lim, JE Lee and EW Seo. 2015. Protective effect of Semisulcospira libertina extract on induced hepatitis in rats. J. Life Sci. 25:539-547.
  31. Reitman S and SA Frankel. 1957. Colorimetric method for determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am. J. Clin. Pathol. 28:56-63.
  32. Shin JK, HY Kim and SM Lee. 2010. Protective effect of Gardenia jasminoides against carbon tetrachloride-induced acute hepatotoxicity. Yakhak Hoeji 54:55-61.
  33. Simeonova PP, RM Gallucci, T Hulderman, R Wilson, C Kommineni, M Rao and MI Luster. 2001. The role of tumor necrosis factor-alpha in liver toxicity, inflammation, and fibrosis induced by carbon tetrachloride. Toxicol. Appl. Pharmacol. 177:112-120.
  34. Wroblewskin F and JS LaDue. 1955. Lactic dehydrogenase activity in blood. Proc. Soc. Exp. Biol. Med. 90:210-213.
  35. Zieve L, WR Anderson and R Dozeman. 1988. Hepatic regenerative enzyme activity after diffuse injury with galactosamine: relationship to histologic alteration. J. Lab. Clin. Med. 112:575-582.