Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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Effect of Feeding on Postlarvae of Pacific White Shrimp, Litopenaeus vannamei during the Acclimation Process to Low Salinities in Seawater

해수 저염분 순치과정에서 먹이섭취가 흰다리새우, Litopenaeus vannamei 유생에 미치는 영향

  • Kim, Su Kyoung (West Sea Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Shim, Na Young (West Sea Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Cho, Ji-Hyun (West Sea Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Kim, Jong Hyun (West Sea Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Kim, Su-Kyoung (West Sea Fisheries Research Institute, National Institute of Fisheries Science)
  • 김수경 (국립수산과학원 서해수산연구소) ;
  • 심나영 (국립수산과학원 서해수산연구소) ;
  • 조지현 (국립수산과학원 서해수산연구소) ;
  • 김종현 (국립수산과학원 서해수산연구소) ;
  • 김수경 (국립수산과학원 서해수산연구소)
  • Received : 2018.03.20
  • Accepted : 2018.08.14
  • Published : 2018.09.30
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Abstract

This study focused on the effects of feeding on postlarvae of shrimp, Litopenaeus vannamei, during the identified acclimation time to low salinity. A total of 5 different salinity groups with or without feeding (32, 24, 16, 8, and 2 psu, 1 liter, triplicates) were prepared, and 30 shrimp were settled at PL21 (postlarvae) and placed in each group. After 24 hours of the experimentation process, the survival rate of the fed and starved groups was observed to be lower in the 2 psu group compared to other salinity groups, with the rate of 86.6% and 81.1%, respectively. The condition index of glucose and triglyceride, which are important factors for osmoregulation and as energy sources, was 4.2-7.6 times and 2.7-3.4 times higher in the fed groups than the starved groups at all the levels of salinities. The creatine level increased by 1.1-1.5 times in the starved groups as compared to the fed groups. Likewise, the activity of all the digestive enzymes like, lipase, ${\alpha}$-amylase, trypsin, and alkaline protease were clearly higher in the fed groups (ANOVA, p<0.05). Apparently, it was observed that feeding is effective for the postlarvae of shrimp, which shows a characteristic fast metabolism and larval development, during the acclimation period to low salinity.

저염분 순치기간에 먹이를 섭취함으로서 얻을 수 있는 초기 유생의 생리적 변화는 다음과 같이 요약할 수 있다. 하루 동안의 순치기간에 염분농도에 따른 유생의 생존율은 차이를 보였지만 각 염분구간별 먹이섭취 유무에 따른 생존효과는 유사하였다. 그러나 먹이섭취 시 유생이 에너지원으로 활용할 수 있는 cholesterol, triglyceride의 증가가 있었으며 삼투압에 영향을 미칠 수 있는 glucose 농도가 유의적으로 증가하였다. 또한 저염분 순치기간 동안에 스트레스, 조직손상 및 대사작용의 지표가 되는 BUN과 creatine의 감소가 있었다. 먹이섭취로 인한 소화효소의 활성이 먹이를 섭취한 실험구 모두에서 증가하였다. 그러므로 유생발달과 함께 중요한 소화, 순환, 생식기관이 형성되며 탈피 성장을 위한 신진대사가 빠르게 진행되는 유생시기에 저염분 순치가 효율적으로 이루어져 향후 지속적인 성장을 유도하기 위해서는 먹이를 섭취하는 것이 더 효과적인 것으로 판단되었다.

Keywords

References

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