생체재료학회지 (Biomaterials Research)

  • 제22권2호
  • /
  • Pages.142-149
  • /
  • 2018
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  • 1226-4601(pISSN)
  • /
  • 2055-7124(eISSN)
한국생체재료학회 (Korean Society for Biomaterials)
권호 표지
DOI QR코드

DOI QR Code

Fabrication and optimization of Nanodiamonds-composited poly(ε-caprolactone) fibrous matrices for potential regeneration of hard tissues

  • Ahn, Guk Young (Department of Biotechnology, The Catholic University of Korea) ;
  • Ryu, Tae-Kyung (Department of Biotechnology, The Catholic University of Korea) ;
  • Choi, Yu Ri (Department of Biotechnology, The Catholic University of Korea) ;
  • Park, Ju Ri (Department of Biotechnology, The Catholic University of Korea) ;
  • Lee, Min Jeong (Department of Biotechnology, The Catholic University of Korea) ;
  • Choi, Sung-Wook (Department of Biotechnology, The Catholic University of Korea)
  • 투고 : 2018.04.10
  • 심사 : 2018.05.20
  • 발행 : 2018.06.01
⟨ 이전 논문 다음 논문 ⟩

초록

Background: Electrospun fibrous matrices are of great importance for tissue engineering and drug delivery device. However, relatively low mechanical strength of the fibrous matrix is one of the major disadvantages. NDs with a positive charge were selected to enhance the mechanical property of a composited fibrous matrix by inducing the intermolecular interaction between NDs and polymer chain. We prepared ND-composited poly ($\varepsilon$-caprolactone) (PCL) fibrous matrices by electrospinning and evaluated their performance in terms of mechanical strength and cell behaviors. Methods: A predetermined amounts of NDs (0.5, 1, 2 and 3 wt%) were added into PCL solution in a mixture of chloroform and 2,2,2-trifluoroethanol (8:2). ND-composited PCL (ND/PCL) fibrous matrices were prepared by electrospinning method. The tensile properties of the ND/PCL fibrous matrices were analyzed by using a universal testing machine. Mouse calvaria-derived preosteoblast (MC3T3-E1) was used for cell proliferation, alkaline phosphatase (ALP) assay, and Alizarin Red S staining. Results: The diameters of the fibrous matrices were adjusted to approximately $1.8{\mu}m$ by changing process variables. The intermolecular interaction between NDs and PCL polymers resulted in the increased tensile strength and the favorable interfacial adhesion in the ND/PCL fibrous matrices. The ND/PCL fibrous matrix with 1 wt% of ND had the highest tensile strength among the samples and also improved proliferation and differentiation of MC3T3-E1 cells. Conclusions: Compared to the other samples, the ND/PCL fibrous matrix with 1 wt% of ND concentration exhibited superior performances for MC3T3 cells. The ND/PCL fibrous matrix can be potentially used for bone and dental tissue engineering.

키워드

과제정보

연구 과제 주관 기관 : National Research Foundation of Korea (NRF), Korea Health Industry Development Institute (KHIDI)

참고문헌

  1. Yang Y, Zhu X, Cui W, Li X, Jin Y. Eletrospun composite mates of poly [(D,Llactide) glycolide] and collagen with high porosity as potential scaffolds for skin tissue engineering. Macromol Mater Eng. 2009;294(9):611-9. https://doi.org/10.1002/mame.200900052
  2. Son HY, Ryu JH, Lee H, Nam YS. Silver-polydopamine hybrid coatings of electrospun poly(vinyl alcohol) nanofibers. Macromol Mater Eng. 2013; 298(5):547-54. https://doi.org/10.1002/mame.201200231
  3. Li W, Laurencin CT, Caterson EJ, Tuan RS, Ko FK. Electrospun nanofibrous structure: a novel scaffold for tissue engineering. J Biomed Mater Res. 2002; 60(4):613-21. https://doi.org/10.1002/jbm.10167
  4. Lin HM, Lin YH, Hsu FY. Preparation and characterization of mesoporous bioactive glass/polycaprolactone nanofibrous matrix for bone tissues engineering. Mater Sci Mater Med. 2012;23(11):2619-30. https://doi.org/10.1007/s10856-012-4734-z
  5. Lim SK, Lee SK, Hwang SH, Kim H. Photocatalytic deposition of silver nanoparticles onto organic/inorganic composite nanofibers. Macromol Mater Eng. 2006;291(10):1265-70. https://doi.org/10.1002/mame.200600264
  6. Zhang Y, Wang R. Fabrication of novel polyetherimide-fluorinated silica organic-inorganic composite hollow fiber membranes intended for membrane contactor application. J Membrane Sci. 2013;443:170-80. https://doi.org/10.1016/j.memsci.2013.04.062
  7. Zhang Y, Venugopal J, El-Turki RA, Ramakrishna S, Su B, Lim CT. Elctrospun biomimetic nanocomposite nanofibers of hydroxyapatite/chitosan for bone tissue engineering. Biomaterials 2008;29(32):4314-4322. https://doi.org/10.1016/j.biomaterials.2008.07.038
  8. Li C, Vepari C, Jin HJ, Kim HJ, Kaplan DL. Electrospun silk-BMP-2 scaffolds for bone tissue engineering. Biomaterials. 2006;27(16):3115-24. https://doi.org/10.1016/j.biomaterials.2006.01.022
  9. Ma X, He Z, Han F, Zhong Z, Chen L, Li B. Preparation of collagen/hydroxyapatite/alendronate hybrid hydrogels as potential scaffolds for bone regeneration. Colloids Surf B Biointerfaces. 2016;143:81-7. https://doi.org/10.1016/j.colsurfb.2016.03.025
  10. Yang F, Wolke JGC, Jansen JA. Biomimetic calcium phosphate coating on electrospun poly($\varepsilon$-caprolactone) scaffolds for bone tissue engineering. Chem Eng J. 2008;137(1):154-61. https://doi.org/10.1016/j.cej.2007.07.076
  11. Rajzer I, Menaszek E, Kwiatkowski R, Planell JA, Castano O. Electrospun gelatin/poly($\varepsilon$-caprolactone) fibrous scaffold modified with calcium phosphate for bone tissue engineering. Mater Sci Eng C Mater. 2014;44:183-90. https://doi.org/10.1016/j.msec.2014.08.017
  12. Cao H, Kuboyama N. A biodegradable porous composite scaffold of PGA/${\beta}$-TCP for bone tissue engineering. Bone. 2010;46(2):386-95. https://doi.org/10.1016/j.bone.2009.09.031
  13. Liu Z, Robinson JT, Tabakman SM, Yang K, Dai H. Carbon materials for drug delivery & cancer therapy. Mater Today. 2011;14(7-8):316-23. https://doi.org/10.1016/S1369-7021(11)70161-4
  14. Liu Z, Liang XJ. Nano-carbons as Theranostics. Theranostics. 2012;2(3):235-7. https://doi.org/10.7150/thno.4156
  15. Depan D, Girase B, Shah JS, Misra RDK. Structure-process-property relationship of the polar graphene oxide-mediated cellular response and stimulated growth of osteoblasts on hybrid chitosan network structure nanocomposite scaffolds. Acta Biomater. 2011;7(9):3432-45. https://doi.org/10.1016/j.actbio.2011.05.019
  16. Pan L, Pei X, He R, Wan Q, Wang J. Multiwall carbon nanotubes/polycaprolactone composites for bone tissue engineering application. Colloids Surf B Biointerfaces. 2012;93:226-34. https://doi.org/10.1016/j.colsurfb.2012.01.011
  17. Mochalin VN, Shenderova O, Ho D, Gogotsi Y. The properties and applications of nanodiamonds. Nat Nanotechnol. 2011;7:11-23.
  18. Liu JH, Yang ST, Chen XX, Wang H. Fluorescent carbon dots and nanodiamonds for biological imaging: preparation, application, pharmacokinetics and toxicity. Curr Drug Metab. 2012;13(8):1046-56. https://doi.org/10.2174/138920012802850083
  19. Zhu Y, Li Z, Li W, Zhang Y, Yang X, Chen N, Sun Y, Zhao Y, Fan C, Huang Q. The biocompatibility of nanodiamonds and their application in drug delivery systems. Theranostics. 2012;2(3):302-12. https://doi.org/10.7150/thno.3627
  20. Grausova L, Bacakova L, Kromka A, Potocky S, Vanecek M, Nesladek M, Lisa V. Nanodiamond as promising material for bone tissue engineering. J Nanosci Nanotechnol. 2009;9(6):3524-34. https://doi.org/10.1166/jnn.2009.NS26
  21. Zhang Q, Mochalin VN, Neitzel I, Knoke IY, Han J, Klug CA, Zhou JG, Lelkes PI, Gogotsi Y. Fluorescent PLLA-nanodiamond composites for bone tissue engineering. Biomaterials. 2011;32(1):87-94. https://doi.org/10.1016/j.biomaterials.2010.08.090
  22. Ryu TK, Kang RH, Jeong KY, Jun DR, Koh JM, Kim D, Bae SK, Choi SW. Bonetargeted delivery of nanodiamond-based drug carriers conjugated with alendronate for potential osteoporosis treatment. J Control Release. 2016; 232:152-60. https://doi.org/10.1016/j.jconrel.2016.04.025
  23. Kakade MV, Givens S, Gardner K, Lee KH. Electric field induced orientation of polymer chains in macroscopically aligned electrospun polymer nanofibers. J Am Chem Soc. 2007;129(10):2777-82. https://doi.org/10.1021/ja065043f
  24. Kwon GW, Kailash CG, Jung KH, Kang IK. Lamination of microfibrous PLGA fabric by electrospinning a layer of collagen-hydroxyapatite composite nanofibers for bone tissue engineering. Biomater Res. 2017;21:11. https://doi.org/10.1186/s40824-017-0097-3
  25. Lee JB, Ko YG, Cho D, Park WH, Kwon OH. Modification and optimization of electrospun gelatin sheets by electron beam irradiation for soft tissue engineering. Biomater Res. 2017;21:14. https://doi.org/10.1186/s40824-017-0100-z
  26. Choi HJ, Lee JJ, Park YJ, Shin JW, Sung HJ, Shin JW, Wu Y, Kim JK. MG-63 osteoblast-like cell proliferation on auxetic PLGA scaffold with mechanical stimulation for bone tissue regeneration. Biomater Res. 2016;20:33. https://doi.org/10.1186/s40824-016-0080-4
  27. Cha SH, Lee HJ, Koh WG. Study of myoblast differentiation using multidimensional scaffolds consisting of nano and micropatterns. Biomater Res. 2017;21(1)
  28. Kim JY, Kim HD, Park JH, Lee ES, Kim EG, Lee SH, Yang JK, Lee YS, Hwang NS. Enhanced osteogenic commitment of murine mesenchymal stem cells on graphene oxide substrate. Biomater Res. 2018;22(1)
  29. Ju YM, Choi JS, Atala A, Yoo JJ, Lee SJ. Bilayered scaffold for engineering cellularized blood vessels. Biomaterials. 2010;31(15):4313-21. https://doi.org/10.1016/j.biomaterials.2010.02.002
  30. Han F, Jia X, Dai D, Yang X, Zhao J, Zhao Y, Fan Y, Yuan X. Performance of a multilayered small-diameter vascular scaffold dual-loaded with VEGF and PDGF. Biomaterials. 2013;34(30):7302-13. https://doi.org/10.1016/j.biomaterials.2013.06.006
  31. Pham QP, Sharma U, Mikos AG. Electrospun poly($\varepsilon$-caprolactone) microfiber and multilayer nanofiber/microfiber scaffolds: characterization of scaffolds and measurement of cellular infiltration. Biomacromolecules. 2006;7(10): 2796-805. https://doi.org/10.1021/bm060680j
  32. Kumbar SG, Nukavarapu SP, James R, Nair LS, Laurencin CT. Electrospun poly(lactic acid-co-glycolic acid) scaffolds for skin tissue engineering. Biomaterials. 2008;29(30):4100-7. https://doi.org/10.1016/j.biomaterials.2008.06.028
  33. Xia Y, Lu Y. Fabrication and properties of conductive conjugated polymers/silk fibroin composite fibers. Compos Sci Technol. 2008;68(6):1471-9. https://doi.org/10.1016/j.compscitech.2007.10.044
  34. Cai N, Dai Q, Wang Z, Luo X, Xue Y, Yu F. Preparation and properties of nanodiamond/poly(lactic acid) composite nanofiber scaffolds. Fiber Polym. 2014;15(12):2544-52. https://doi.org/10.1007/s12221-014-2544-2
  35. Wang Z, Cai N, Zhao D, Xu J, Dai Q, Xue Y, Luo X, Yang Y, Yu F. Mechanical reinforcement of electrospun water-soluble polymer nanofibers using nanodiamonds. Polym Compos. 2013;34(10):1735-44. https://doi.org/10.1002/pc.22577
  36. Daniels AU, Chang MKO, Andriano KP. Mechanical properties of biodegradable polymers and composites proposed for internal fixation of bone. J Appl Biomater. 1990;1(1):57-78. https://doi.org/10.1002/jab.770010109
  37. Nitya G, Nair GT, Mony U, Chennazhi KP, Nair SV. In vitro evaluation of electrospun PCL/nanoclay composite scaffold for bone tissue engineering. J Mater Sci Mater Med. 2012;23(7):1749-61. https://doi.org/10.1007/s10856-012-4647-x
  38. Li L, Li G, Jiang J, Liu X, Luo L, Nan K. Electrospun fibrous scaffold of hydroxyapatite/poly ($\varepsilon$-caprolactone) for bone regeneration. Mater Sci Mater Med. 2012;23(7):547-54. https://doi.org/10.1007/s10856-011-4495-0
  39. Damien E, Price JS, Lanyon LE. Mechanical strain stimulates osteoblast proliferation through the estrogen receptor in males as well as females. J Bone Miner Res. 2000;15(11):2169-77. https://doi.org/10.1359/jbmr.2000.15.11.2169
  40. Yan YX, Gong YW, Guo Y, Lv Q, Guo C, Zhuang Y, Zhang Y, Li R, Zhang X. Mechanical strain regulates osteoblast proliferation through integrinmediated ERK activation. PLoS One. 2012;7(4):e35709. https://doi.org/10.1371/journal.pone.0035709
  41. Koike M, Shimokawa H, Kanno Z, Ohya K, Soma K. Effects of mechanical strain on proliferation and differentiation of bone marrow stromal cell line ST2. J Bone Miner Metab. 2005;23(3):219-25. https://doi.org/10.1007/s00774-004-0587-y
  42. Ohgaki M, Kizuki T, Katsura M, Yamashita K. Manipulation of selective cell adhesion and growth by surface charges of electrically polarized hydroxyapatite. J Biomed Mater Res. 2001;57(3):366-73. https://doi.org/10.1002/1097-4636(20011205)57:3<366::AID-JBM1179>3.0.CO;2-X

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