Mass Spectrometry Letters

Korean Society for Mass Spectrometry (한국질량분석학회)
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Free Radical Initiated Peptide Sequencing Using MALDI-TOF/TOF Mass Spectrometry

  • Received : 2018.06.02
  • Accepted : 2018.06.12
  • Published : 2018.06.30
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Abstract

In this study, matrix-assisted laser desorption/ionization (MALDI) was applied to the TEMPO-assisted FRIPS for the first time. We found that 3-HPA is the optimal matrix for the analysis of p-TEMPO-Bz-Sc-peptides, which gives minimal precursor fragmentations. MALDI-TOF/TOF experiments on p-TEMPO-Bz-Sc-peptides yielded mainly $[a_n+H]^+$, $[z_n+H]^+$, and $[y_n]^+-type$ products, indicating that radical-driven peptide fragmentation occurs in MALDI-TOF/TOF-MS.

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References

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