Textile Coloration and Finishing (한국염색가공학회지)

The Korean Society of Dyers and Finishers (한국염색가공학회)
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Control of Molecular Weight and Terminal Groups of Poly(3-hydroxybutyrate) in Bio-synthesis

미생물 합성에 의한 poly(3-hydroxybutyrate)의 분자량과 말단관능기 제어

  • Lee, Chan Woo (Department of Innovative Industrial Technology, Hoseo University)
  • 이찬우 (호서대학교 바이오산업학부)
  • Received : 2018.03.27
  • Accepted : 2018.06.07
  • Published : 2018.06.27
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Abstract

In the bio-synthesis of poly(3-hydroxybutyrate)(PHB), which is a kind of poly(3-hydroxyalkanoate)(PHA), aimed to control the low molecular weight of PHB and obtain a telechelic PHB. As a result of incubation of R. eutropha at $30^{\circ}C$ with ethylene glycol added as a chain transfer agent, PHB content on the dry cell weight increased up to 24h, however, it decreased after that, and the molecular weight of PHB increased from 9h to 12h, and then, decreased up to 72h. The decrease of the content and the molecular weight of PHB indicates that PHB was decomposed as an energy source in bacterial cells and was incorporated into metabolic pathways. $^1H-NMR$ of the obtained PHB after incubation for 72h was measured to determine the terminal groups of the PHB during incubation. As the results of $^1H-NMR$ measurement, the peaks derived from ethylene glycol in both terminals of PHB were observed. Which indicate that the terminal reaction was caused by the addition of ethylene glycol, and that telechelic PHB having hydroxyl group at the both terminals where molecular weight was controlled was successfully synthesized.

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References

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