Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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In Vitro Cytotoxicity against Human Cancer Cell and 3T3-L1 Cell, Total Polyphenol Content and DPPH Radical Scavenging of Codonopsis lanceolata according to the Concentration of Ethanol Solvent

  • Boo, Hee-Ock (WELLPHYTO Co. Ltd., BI Center, GIST) ;
  • Park, Jeong-Hun (WELLPHYTO Co. Ltd., BI Center, GIST) ;
  • Lee, Moon-Soon (Department of Industrial Plant Science & Technology, Chungbuk National University) ;
  • Kwon, Soo-Jeong (Faculty of Food Nutrition and Cookery, Woosong Information College) ;
  • Kim, Hag-Hyun (Faculty of Food Nutrition and Cookery, Woosong Information College)
  • Received : 2018.06.04
  • Accepted : 2018.06.14
  • Published : 2018.06.30
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Abstract

This study was executed to evaluate the phenolic content, DPPH radical scavenging rate, and the cytotoxic effect in human cancer cell, 3T3-L1 cell from C. lanceolata extracts at various ethanol concentration. Total polyphenol and flavonoid content of the C. lanceolata at various ethanol concentration showed the high amount in 70%, 100% ethanol extract. The DPPH radical scavenging activity progressively increased in a dose-dependent manner, and showed the highest in 100% ethanol extract. The cytotoxic effect against human cancer cell of the C. lanceolata was higher in 50% and 70% ethanol extracts. In particular, the cytotoxic effect in MCF-7 cell was relatively higher than in other cells. The $IC_{50}$ (concentration causing 50% cell death) value showed the highest on MCF-7 cell ($538.39{\mu}g/m{\ell}$ in 70% ethanol extract, and exhibited significant activity against Hela cell ($637.87{\mu}g/m{\ell}$, Calu-6 cell ($728.64{\mu}g/m{\ell}$. The extract of 70% ethanol at $1,000{\mu}g/m{\ell}$ exhibited a pronounced cytotoxic effect on 3T3-L1 cell comparable to that of the other extracts, and reduced in a concentration-dependent manner.

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References

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