Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Plant Growth-Promoting Rhizobacteria Improved Salinity Tolerance of Lactuca sativa and Raphanus sativus

  • Hussein, Khalid Abdallah (Department of Botany and Microbiology, Faculty of Science, Assiut University) ;
  • Joo, Jin Ho (Department of Biological Environment, Kangwon National University)
  • Received : 2017.12.07
  • Accepted : 2018.03.27
  • Published : 2018.06.28
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Abstract

Salinity stress is an important environmental problem that adversely affects crop production by reducing plant growth. The impacts of rhizobacterial strains to alleviate salinity stress on the germination of Lactuca sativa and Raphanus sativus seeds were assessed using different concentrations of NaCl. Plant growth-promoting rhizobacteria (PGPR) strains were also examined to improve the early germination of Chinese cabbage seeds under normal conditions. Lactobacillus sp. and P. putida inoculation showed higher radicle lengths compared with non-inoculated radish (Raphanus sativus) seeds. LAP mix inoculation increased the radicle length of lettuce (Lactuca sativa) seedlings by 2.0 and 0.5 cm at salinity stress of 50 and 100 mM NaCl concentration, respectively. Inoculation by Azotobacter chroococcum significantly increased the plumule and radicle lengths of germinated seeds compared with non-inoculated control. A. chroococcum increased the radicle length relative to the uninoculated seeds by 4.0, 1.0, and 1.5 cm at 50, 100, and 150 mM NaCl concentration, respectively. LAP mix inoculation significantly improved the radicle length in germinated radish seeds by 7.5, 1.3, 1.2, and 0.6 cm under salinity stress of 50, 100, 150, and 200 mM NaCl concentration, respectively. These results of this study showed that PGPR could be helpful to mitigate the salinity stress of different plants at the time of germination.

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References

  1. Shahbaz M, Ashraf M. 2013. Improving salinity tolerance in cereals. Crit. Rev. Plant Sci. 32: 237-249. https://doi.org/10.1080/07352689.2013.758544
  2. Donohue K. 2005. Seeds and seasons: interpreting germination timing in the field. Seed Sci. Res. 15: 175-187. https://doi.org/10.1079/SSR2005208
  3. Yang J , Kloepper JW, Ryu CM. 2010. Rhizosphere b acteria help plants tolerate abiotic stress. Trends Plant Sci. 14: 1-4.
  4. Bybordi A, Tabatabaei SJ, Ahmadev A. 2010. Effect of salinity on the growth and peroxidase and IAA oxidase activities in canola. J. Food Agric. Environ. 8: 109-112.
  5. Bybordi A. 2010. The influence of salt stress on seed germination, growth and yield of canola cultivars. Not. Bot. Horti Agrobot. Cluj Napoca 38: 128-133.
  6. Glick BR, Todorovic B, Czarny J, Cheng Z, Duan J, Conkey BM. 2007. Promotion of plant growth by bacterial ACC deaminase. Crit. Rev. Plant Sci. 26: 227-242. https://doi.org/10.1080/07352680701572966
  7. Mishra M, Kumar U, Mishra PK, Prakash V. 2010. Efficiency of plant growth promoting rhizobacteria for the enhancement of Cicer arietinum L. growth and germination under salinity. Adv. Biol. Res. 4: 92-96.
  8. Glick RB. 2012. Plant growth-promoting bacteria: mechanisms and applications. Scientifica 10: 6064.
  9. Turner TR, Ramakrishnan K, Walshaw J, Heavens D, Alston M, Swarbeck D. 2013. Comparative metatranscriptomics reveals kingdom level changes in the rhizosphere microbiome of plants. ISME J. 7: 2248-2258. https://doi.org/10.1038/ismej.2013.119
  10. Hmaeid N, Metoui O, Wali M, Zorrig W, Abdelly C. 2014. Comparative effects of rhizobacteria in promoting growth of Hordeum maritimum L. plants under salt stress. J. Plant Biol. Res. 3: 37-50.
  11. Balloi A, Rolli E, Marasco R. 2010. The role of microorganisms in bioremediation and phytoremediation of polluted and stressed soils. Agrochimica 54: 353-369.
  12. Bacilio M, Rodriguez H, Moreno M. 2004. Mitigation of salt stress in wheat seedlings by gfp-tagged Azospirillum lipoferum. Biol. Fertil. Soils 40: 188-193.
  13. Hussein KA, Joo JH. 2015. Isolation and characterization of rhizomicrobial isolates for phosphate solubilization and indole acetic acid production. J. Korean Soc. Appl. Biol. Chem. 58: 847-855. https://doi.org/10.1007/s13765-015-0114-y
  14. McFarland J. 1907. Nephelometer: an instrument for media used for estimating the number of bacteria in suspensions used for calculating the opsonic index and for vaccines. J. Am. Med. Assoc. 14: 1176-1178.
  15. Kim K, Hwang S, Saravanan VS, Sa T. 2012. Effect of Brevibacterium iodinum RS16 and Methylobacterium oryzae CBMB20 inoculation on seed germination and early growth of maize and sorghum-sudan grass hybrid seedling under different salinity levels. Korean J. Soil Sci. Fert. 45: 51-58. https://doi.org/10.7745/KJSSF.2012.45.1.051
  16. Kader MA. 2005. A comparison of seed germination calculation formulae and the associated interpretation of resulting data. J. Proc. R. Soc. New South Wales 138: 65-75.
  17. SAS Institute Inc. 2009. SAS, SAS/STAT 9.1 User's Guide. SAS Institute Inc., Cary, NC, USA.
  18. Grover A, Aggarwal PK, Kapoor A, Katiyar-Agarwal S, Agarwal M, Chandramouli A. 2011. Addressing abiotic stresses in agriculture through transgenic technology. Curr. Sci. 84: 355-367.
  19. Sadeghi A, Karimi E, Dahazi PA, Javid MG, Dalvand Y, Askari H. 2012. Plant growth promoting activity of an auxin and siderophore producing isolate of Streptomyces under saline soil condition. World J. Microbiol. Biotechnol. 28: 1503-1509. https://doi.org/10.1007/s11274-011-0952-7
  20. Parida AK, Das AB. 2005. Salt tolerance and salinity effects on plants: a review. Ecotoxicol. Environ. Saf. 60: 324-349. https://doi.org/10.1016/j.ecoenv.2004.06.010
  21. Hardoim PR, van Overbeek SV, van Elsas JD. 2008. Properties of bacterial endophytes and their proposed role in plant growth. Trends Microbiol. 16: 463-471. https://doi.org/10.1016/j.tim.2008.07.008
  22. Dimkpa C, Weinand T, Asch F. 2009. Plant rhizobacteria interactions alleviate abiotic stress conditions. Plant Cell Environ. 32: 1682-1694. https://doi.org/10.1111/j.1365-3040.2009.02028.x
  23. Dubois M, Broeck LV, Inze D. 2018. The pivotal role of ethylene in plant growth. Trends Plant Sci. 23: 311-323. https://doi.org/10.1016/j.tplants.2018.01.003
  24. Dubois M, Broeck LV, Inze D. 2017. Time of day determines Arabidopsis transcriptome and growth dynamics under mild drought. Plant Cell Environ. 40: 180-189. https://doi.org/10.1111/pce.12809
  25. Chen L, Dodd IC, Theobald JC, Belimov AA, Davies WJ. 2013. The rhizobacterium Variovorax paradoxus 5C-2, containing ACC deaminase, promotes growth and development of Arabidopsis thaliana via an ethylene-dependent pathway. J. Exp. Bot. 64: 1565-1573. https://doi.org/10.1093/jxb/ert031
  26. Onofre-Lemus J, Hernandez-Lucas I, Girard L, Caballero- Mellado J. 2009. ACC (1-aminocyclopropane-1-carboxylate) deaminase activity, a wide spread trait in Burkholderia species, and its growth-promoting effect on tomato plants. Appl. Environ. Microbiol. 75: 6581-6590. https://doi.org/10.1128/AEM.01240-09
  27. Saleem M, Arshad M, Hussain S, Bhatti AS. 2007. Perspective of plant growth promoting rhizobacteria (PGPR) containing ACC deaminase in stress agriculture. J. Ind. Microbiol. Biotechnol. 34: 635-648. https://doi.org/10.1007/s10295-007-0240-6
  28. Arora NK, Tewari S, Singh S, Lal N, Maheshwari DK. 2012. PGPR for protection of plant health under saline conditions, pp. 239-258. In Maheshwari DK (ed.). Bacteria in Agrobiology: Stress Management. Springer, Berlin.
  29. Gerhardt KE, Greenberg BM, Glick BR. 2006. The role of ACC deaminase in facilitating the phytoremediation of organics, metals and salt. Curr. Trends Microbiol. 2: 61-72.
  30. Cassan F, Maiale S, Masciarelli O, Vidal A, Luna V, Ruiz O. 2009. Cadaverine production by Azospirillum brasilense and its possible role in plant growth promotion and osmotic stress mitigation. Eur. J. Soil Biol. 45: 12-19. https://doi.org/10.1016/j.ejsobi.2008.08.003
  31. Xie SS, Wu HJ, Zang HY, Wu LM, Zhu QQ, Gao XW. 2014. Plant growth promotion by spermidine-producing Bacillus subtilis OKB105. Mol. Plant Microbe. Interact. 27: 655-663. https://doi.org/10.1094/MPMI-01-14-0010-R
  32. Jha Y, Subramanian RB. 2014. PGPR regulate caspase-like activity, programmed cell death, and antioxidant enzyme activity in paddy under salinity. Physiol. Mol. Biol. Plant 20: 201-207. https://doi.org/10.1007/s12298-014-0224-8
  33. Dodd IC, Perez-Alfocea F. 2012. Microbial amelioration of crop salinity stress. J. Exp. Bot. 63: 3415-3428. https://doi.org/10.1093/jxb/ers033
  34. Upadhyay SK, Singh DP. 2015. Effect of salt-tolerant plant growth-promoting rhizobacteria on wheat plants and soil health in a saline environment. Plant Biol. 17: 288-293. https://doi.org/10.1111/plb.12173
  35. Barriuso J, Ramos Solano B, Gutierrez Manero FJ. 2008. Protection against pathogen and salt stress by four plant growth-promoting rhizobacteria isolated from Pinus sp. on Arabidopsis thaliana. Phytopathology 98: 666-672. https://doi.org/10.1094/PHYTO-98-6-0666
  36. Yildrim E, Donmez MF, Turan M. 2008. Use of bioinoculants in ameliorative effects on radish plants under salinity stress. J. Plant Nutr. 31: 2059-2074. https://doi.org/10.1080/01904160802446150

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