생약학회지 (Korean Journal of Pharmacognosy)

  • 제49권2호
  • /
  • Pages.138-144
  • /
  • 2018
  • /
  • 0253-3073(pISSN)
  • /
  • 2288-9299(eISSN)
한국생약학회 (The Korean Society of Pharmacognosy)
권호 표지

방울비짜루 지표성분의 함량분석과 항산화·항염증 활성

Quantitative Analysis of Chemical Composition and In Vitro Anti-oxidant and Anti-inflammatory Properties of Asparagus oligoclonos

  • 이현주 (한약진흥재단 한약자원본부) ;
  • 정다은 (한약진흥재단 한약자원본부) ;
  • 강주은 (한약진흥재단 한약자원본부) ;
  • 심미옥 (한약진흥재단 한약자원본부) ;
  • 성태경 (한약진흥재단 한약자원본부) ;
  • 우경완 (한약진흥재단 한약자원본부) ;
  • 안병관 (한약진흥재단 한약자원본부) ;
  • 정호경 (한약진흥재단 한약자원본부) ;
  • 조현우 (한약진흥재단 한약자원본부)
  • Lee, Hyun Joo (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine) ;
  • Jeong, Da Eun (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine) ;
  • Gang, Ju Eun (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine) ;
  • Sim, Mi-Ok (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine) ;
  • Seong, Tae Kyoung (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine) ;
  • Woo, Kyeong Wan (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine) ;
  • An, Byeongkwan (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine) ;
  • Jung, Ho Kyung (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine) ;
  • Cho, Hyun Woo (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine)
  • 투고 : 2018.02.27
  • 심사 : 2018.05.04
  • 발행 : 2018.06.30
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초록

Asparagus oligoclonos is one of the endemic halophytes used folk medicine in Korea. We isolated the main compound rutin from methanol extracts of A. oligoclonos based on nuclear magnetic resonance and TOF ESI-MS data. We have investigated the quantitative analysis method of main compound using HPLC and the results exhibit that rutin content of A.oligoclonos were 1.816%. To explore anti-oxidant from A. oligoclonos ethanol extracts (AOE), we investigated the antioxidant effects of AOE on $H_2O_2$-induced oxidative stress in RAW 264.7 cell. AOE were reduced $H_2O_2$-induced oxidative stress via enhancement of cell viability, and AOE significantly decreased ROS production depending on concentration. Next, to screen for anti-inflammatory activity of AOE, we investigated the inhibitory effects of AOE in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. AOE had no effect on cell viability at a concentration of $500{\mu}g/mL$. Nitric oxide (NO) production inhibited in a dose-dependent manner. These results suggest that AOE may be a useful antioxidant and anti-inflammatory agent.

키워드

참고문헌

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