DOI QR코드

DOI QR Code

트립신 처리에 따른 적송잎 추출물의 항산화 활성 및 항균 효과

Antioxidant and Antimicrobial Activities of Trypsin-treated Pinus densiflora Ethanol Extract

  • 문기은 (계명대학교 자연과학대학 공중보건학과) ;
  • 박교현 (계명대학교 자연과학대학 공중보건학과) ;
  • 이범주 ((주)케미랜드) ;
  • 김배환 (계명대학교 자연과학대학 공중보건학과)
  • Moon, Ki-Eun (Department of Public Health, College of Natural Sciences, Keimyung University) ;
  • Park, Kyo-Hyun (Department of Public Health, College of Natural Sciences, Keimyung University) ;
  • Lee, Beom Zoo (CHEMLAND Co., Ltd.) ;
  • Kim, Bae-Hwan (Department of Public Health, College of Natural Sciences, Keimyung University)
  • 투고 : 2018.03.28
  • 심사 : 2018.06.20
  • 발행 : 2018.06.28

초록

Objectives: We investigated the antioxidant and antibacterial activities of Pinus densiflora ethanol extracts (PDEE) treated with trypsine as a protease. Methods: Various antioxidant activities were evaluated by measuring total contents of polyphenol and flavonoid, DPPH electron-donating ability and $ABTS^+$ radical scavenging activity of test material. To compare the antibacterial activity, paper disc diffusion assay was performed against two resident bacteria in human skin (Staphylococcus aureus and Staphylococcus epidermidis). Results: As for the total contents of polyphenol and flavonoid, and the electron-donating ability and ABTS+ radical scavenging activity, both PDEE and trypsin-treated Pinus densiflora ethanol extract (T-PDEE) showed high antioxidant activity in dose-dependent manner. And the T-PDEE showed slightly higher activity than PDEE, which indicated protease treatment seemed to affect in antioxidant activity. In the result of paper disc diffusion assay, antibacterial activity was confirmed in all two types of skin resident bacteria. T-PDEE was more active than PDEE and it seems that treatment of protease may increase the antibacterial activity of PDEE. Conclusion: All of these results, we confirmed that treatment of protease to PDEE can increase the antioxidant and antibacterial activities, and it can be explained thought that this would be applicable as a cosmeceutical material in the future.

키워드

참고문헌

  1. Yang SJ, Woo KS, Yoo JS, Kang TS, Noh YH, Lee JS, et al. Change of Korean Ginseng Components with High Temperature and Pressure Treatment. Korean J Food Sci Technol. 2006; 98(4):521-525.
  2. Kwon SC, Choi GH, Hwang JH, Lee KH. Physicochemical Property and Antioxidative Activity of Hot-Water Extracts from Enzyme Hydrolysate of Astragalus membranaceus. J Korean Soc Food Sci Nutr. 2010; 39(3): 406-413. https://doi.org/10.3746/jkfn.2010.39.3.406
  3. Park MK. Effect of Enzymatic Hydrolysis by Proteases on Antioxidant Activity of Chungkukjang. J Korean Soc Food Sci Nutr. 2011; 40(2): 327-333. https://doi.org/10.3746/jkfn.2011.40.2.327
  4. Jang YS, Jeong JM. Antioxidative Effect and Digestive Enzyme Inhibition of Grape Seed Extract (GSE). J Korean Soc Food Sci Nutr. 2010; 39(6): 783-788. https://doi.org/10.3746/jkfn.2010.39.6.783
  5. Kim SY, Lee MH, Jo NR, Park SN. Antibaterial Activity and Skin Moisturizing Effect of Cedrela sinensis A. Juss Shoots Extracts. J Soc Cosmet Scientists Korea. 2010; 36(4): 315-321.
  6. Sung HC, Jung HD, Park KD, Lee WJ, Lee SJ, Kim DW. A Quantitative Culture Study of Staphylococcus aureus in Adolescent and Adult Patients with Atopic Dermatitis using the Contact-plate Sampling Technique. Korean J Dermatol. 2007; 47(7): 673-679.
  7. Yang SA, Kim AY, Pyo BS, Kim SM. Antibacterial and Antibiotic Activity Enhancing Effect of Extract and Fractions from the Root of Rumex japonicus Houtt. Korean J Medicinal Crop Sci. 2017; 25(6): 375-380. https://doi.org/10.7783/KJMCS.2017.25.6.375
  8. Kang SR, Kim YK, Kim SG, Lee SH, Kim MH. The Effect of Pine (pinus densiflora) Needle Extracts on Blood Flow and Serum Lipid Improvement. Journal of Life Science. 2009; 19(4): 508-513. https://doi.org/10.5352/JLS.2009.19.4.508
  9. Kim YS, Shin DH. Volatile components and antibacterial effects of pine needle (Pinus densiflora S. and Z.) extracts. Food Microbiol. 2005; 22: 37-45. https://doi.org/10.1016/j.fm.2004.05.002
  10. Hsu TY, Sheu SC, Liaw ET, Wang TC, Lin CC. Anti-oxidant activity and effect of Pinus morrisonicola Hay. on the survival of leukemia cell line U937. Phytomedicine. 2005; 12: 663-669. https://doi.org/10.1016/j.phymed.2004.03.013
  11. Sanders KM. A Case for Interstitial Cells of Cajal as Pacemakers and Mediators of Neurotransmission in the Gastrointestinal Tract. Gastroenterology. 1996; 111: 492-515. https://doi.org/10.1053/gast.1996.v111.pm8690216
  12. Ward SM, Burns AJ, Torihashi S, Sanders KM. Mutation of the proto?oncogene c?kit blocks development of interstitial cells and electrical rhythmicity in murine intestine. J Physiol. 1994; 480: 91-97. https://doi.org/10.1113/jphysiol.1994.sp020343
  13. Tokutomi N, Maeda H, Tokutomi Y, Sato D, Sugita M, Nishikawa S, et al. Rhythmic Cl-current and physiological roles of the intestinal c-kit-positive cells. Pflugers Arch. 1995; 431: 169-177. https://doi.org/10.1007/BF00410188
  14. Thomson L, Robinson TL, Lee JC, Farraway LA, Hughes MJ, Andrews DW, et al. Interstitial cells of Cajal generate a rhythmic pacemaker current. Nat Med. 1998; 4: 848-851. https://doi.org/10.1038/nm0798-848
  15. Bagchi D, Garg A, Krohn RL, Bagchi M, Bagchi DJ, Balmoori J, et al. Protective Effects of Grape Seed Proanthocyanidins and Selected Antioxidants against TPA-Induced Hepatic and Brain Lipid Peroxidation and DNA Fragmentation, and Peritoneal Macrophage Activation in Mice. Gen Pharmacol. 1998; 30: 771-776. https://doi.org/10.1016/S0306-3623(97)00332-7
  16. Folin O, Denis W. On phosphotungstic-phosphomolybdic compounds as color reagents. J Biol Chem. 1912; 12: 239-243.
  17. Davies R, Massey RC, McWeeny DJ. The catalysis of the N-nitrosation of secondary amines by nitrosophenols. Food Chem. 1980; 6: 115-122. https://doi.org/10.1016/0308-8146(80)90027-8
  18. Blois MS. Antioxidant Determinations by the Use of a Stable Free Radical. nature. 1958; 181: 1199-1200. https://doi.org/10.1038/1811199a0
  19. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. 1999; 26: 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  20. Lee YJ, Kim TY, Chung HW. Protective Effects of Ginkgo Biloba Leaf Extract (GBE) against 1,2,4-benzenetriol Induced Toxicity in Vitro. J Environ Health Sci. 2001; 27: 124-130.
  21. Mo JH, Kim MA, Kang EJ. A Comparative Study of the Physiological Activities of Skin between Codonopsis lanceolata and Fermented C. lanceolata Extracts as Cosmetic Ingredient. Journal of the Korea Soc Beauty and Art. 2013; 14: 49-60.
  22. Kang SH. Identification of Anti-oxidative, Skin Whitening and Anti- wrinkle Constituents from the Leaves of Pinus densiflora and Sargassum Muticum, [dissertation]. [Jeju]: Jeju University; 2015
  23. Kim BY, Lee JH, Kim SY, Lee EJ, Choi CR, Kho YL. Determination of Preservatives in Pharmaceuticals and Personal Care Products. J Environ Health Sci. 2016; 42: 53-60.
  24. Hwang SH. Preservation of Scutellariae Radix Extract for Cosmetics, [dissertation]. [Seoul]: Kyung Hee University; 2009.