Korean Journal of Clinical Laboratory Science (대한임상검사과학회지)

Korean Society for Clinical Laboratory Science (대한임상검사과학회)
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Molecular Characterization and Antimicrobial Susceptibility of Biofilm-forming Acinetobacter baumannii Clinical Isolates from Daejeon, Korea

대전지역에서 분리된 생물막 형성 Acinetobacter baumannii 임상분리주의 분자유전학적 특성과 항균제 감수성양상

  • Sung, Ji Youn (Department of Biomedical Laboratory Science, Far East University,)
  • 성지연 (극동대학교 임상병리학과)
  • Received : 2018.02.14
  • Accepted : 2018.03.13
  • Published : 2018.06.30
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Abstract

The emergence and dissemination of multidrug-resistant (MDR) Acinetobacter baumannii isolates have been reported worldwide, with most of these possessing the ability to form biofilms. Biofilm formation is an important virulence factor associated with the resistance to disinfection and desiccation. This study examined the genetic basis of antimicrobial resistance mechanisms of biofilm-forming A. baumannii clinical isolates. Imaging and quantification of biofilms were performed by a crystal violet assay and 46 biofilm-forming A. baumannii isolates were selected. Subsequently, 16 isolates belonging to different clones were identified using REP-PCR, and detection of the antimicrobial determinants in the isolates was carried out. The 16 isolates included 9 non-MDR and 7 MDR isolates. The mean biomass $OD_{560}$ values of the non-MDR (0.96) and MDR (1.05) isolates differed but this difference was not significant. In this study, most biofilm-forming MDR A. baumannii isolates contained various antimicrobial resistance determinants ($bla_{OXA-23}$, armA, and mutations of gyrA and parC). On the other hand, most biofilm-forming non-MDR A. baumannii isolates did not contain antimicrobial resistance determinants. These results suggest that there is little correlation between the biofilm-forming ability and antimicrobial susceptibility in A. baumannii isolates. In addition, the emergence of MDR A. baumannii clinical isolates is generally caused by mutations of the genes associated with antimicrobial resistance and/or the acquisition of various antimicrobial resistance determinants.

다제내성(multidrug-resistant, MDR) Acinetobacter baumannii 균주의 출현 및 확산이 전 세계적으로 보고되어 왔는데, 이들 대부분은 생물막 형성능력을 가지고 있다. 생물막 형성은 소독 및 건조에 대해 세균이 저항할 수 있도록 해주는 중요한 병독성 인자이다. 본 연구에서는 생물막을 형성하는 A. baumannii 임상 분리주를 대상으로 항균제 내성 기전에 대한 유전적 기초를 조사하였다. 크리스탈 바이올렛 분석법을 통해 생물막 형성능력을 확인한 결과 46균주가 생물막을 형성하는 것으로 나타났다. 이어서 REP-PCR을 수행하여 서로 다른 클론에 속하는 16 균주를 선택한 후 이 균주들을 대상으로 항균제 내성 인자를 검출하였다. 본 연구에서 분리된 9개 non-MDR (0.96) 균주와 7개의 MDR (1.05) 균주의 평균 생물량은 서로 달랐지만, 이 차이는 유의하지 않았다. 대부분의 생물막 형성 MDR 균주는 다양한 항균제 내성인자 ($bla_{OXA-23}$, armA 및 gyrA 및 parC의 돌연변이)를 가지고 있는 것으로 나타났다. 그러나 대부분의 non-MDR 균주는 항균제 내성인자를 포함하지 않는 것으로 나타났다. 우리의 결과는 A. baumannii 균주의 생물막 형성능력이 항균제 감수성과 상관관계가 적음을 시사한다. 또한 MDR A. baumannii 임상 분리주의 출현은 대개 항균제 내성과 관련된 유전자의 돌연변이나 또는 다양한 항균제 내성인자의 획득에 의한 것으로 사료된다.

Keywords

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