The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Changes in γ-Aminobutyric Acid of Bitter Melon (Momordica charantia L.) with Different Cultivation Regions, Harvest Time and Maturation Stages, with Method Validation

재배지역, 수확시기 및 숙기에 따른 여주의 γ-Aminobutyric Acid 함량 변화 및 분석법 검증

  • Lee, Sang Hoon (Dept. of Agrofood Resources, National Academy of Agricultural Sciences, Rural Development Administration) ;
  • Jeong, Yun Sook (Dept. of Agrofood Resources, National Academy of Agricultural Sciences, Rural Development Administration) ;
  • Song, Jin (Dept. of Agrofood Resources, National Academy of Agricultural Sciences, Rural Development Administration) ;
  • Hwang, Kyung-A (Dept. of Agrofood Resources, National Academy of Agricultural Sciences, Rural Development Administration) ;
  • Cho, Soo Muk (Dept. of Agrofood Resources, National Academy of Agricultural Sciences, Rural Development Administration) ;
  • Hwang, In Guk (Dept. of Agrofood Resources, National Academy of Agricultural Sciences, Rural Development Administration)
  • 이상훈 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 정윤숙 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 송진 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 황경아 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 조수묵 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 황인국 (농촌진흥청 국립농업과학원 농식품자원부)
  • Received : 2017.07.07
  • Accepted : 2018.06.05
  • Published : 2018.06.30
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Abstract

This study aimed to investigate the changes in the ${\gamma}$-aminobutyric acid (GABA) content of bitter melon (Momordica charantia L.) cultivated from different regions, with different harvest times and at various maturation stages. Methods for observing the changes in GABA content were validated by determining the specificity, linearity, limit of detection (LOD), limit of quantification (LOQ), and precision and accuracy using the HPLC-FLD system. Results showed high linearity in the calibration curve with a coefficient of correlation ($R^2$) of 0.9999. The LOD and LOQ values for GABA were 0.29 and $0.87{\mu}g/mL$, respectively. The relative standard deviations for intra- and inter-day precision of GABA were less than 5%. The recovery rate of GABA was in the range of 98.77% to 100.50%. The average content of GABA was 0.93 mg/g and Cheongju showed highest GABA content of 1.88 mg/g. As the time of harvest increased from May to September, the GABA content decreased from 1.56 to 0.86 mg/g. Also, maturation of the bitter melon fruit was associated with a decreased in GABA content.

Keywords

References

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