Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Draft genome sequence of lytic bacteriophage CP3 infecting anaerobic bacterial pathogen Clostridium perfringens

혐기성 병원균 Clostridium perfringens를 감염시키는 용균 박테리오파지 CP3의 유전체 염기서열 초안

  • Kim, Youngju (Optipharm Inc.) ;
  • Ko, Seyoung (Department of Genetic Engineering and Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University) ;
  • Yeon, Young Eun (Optipharm Inc.) ;
  • Le, Hoa Thi (Department of Genetic Engineering and Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University) ;
  • Han, Beom Ku (Optipharm Inc.) ;
  • Kim, Hyunil (Optipharm Inc.) ;
  • Oh, Chang-Sik (Department of Horticultural Biotechnology, College of Life Sciences, Kyung Hee University) ;
  • Kim, Donghyuk (Department of Genetic Engineering and Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University)
  • Received : 2018.02.09
  • Accepted : 2018.04.23
  • Published : 2018.06.30
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Abstract

Clostridium perfringens is a Gram-positive, rod-shaped, anaerobic, spore-forming pathogenic bacterium, which belongs to the Clostridiaceae family. C. perfringens causes diseases including food poisoning in vertebrates and intestinal tract of humans. Bacteriophages that can kill target bacteria specifically have been considered as one of control methods for bacterial pathogens. Here, we report a draft genome sequence of the bacteriophage CP3 effective to C. perfringens. The phage genome comprises 52,068 bp with a G + C content of 34.0%. The draft genome has 74 protein-coding genes, 29 of which have predicted functions from BLASTp analysis. Others are conserved proteins with unknown functions. No RNAs were found in the genome.

Clostridium perfringens는 그람 양성, 막대 모양, 혐기성, 포자 형성을 하는 병원균으로서 Clostridiaceae과에 속한다. C. perfringens는 인간의 장관과 척추동물 내에서 식중독을 포함하는 질병을 유발한다. 높은 특이성으로 목표 세균을 죽이는 박테리오파지는 병원세균을 제어하는 방법들 중 하나로 여겨져 왔다. 본 연구에서는 C. perfringens를 감염시킬 수 있는 박테리오 파지 CP3의 유전체 염기서열 초안을 보고한다. 본 박테리오파지의 G + C 비율은 34.0%이며, 52,068 bp로 구성된 유전체 DNA를 지니고 있었다. 이 유전체는 74개의 단백질 유전자를 포함하고 있었으며, RNA는 확인되지 않았다.

Keywords

References

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